Cu-MOFs/GOx Bifunctional Probe-Based Synergistic Signal Amplification Strategy: Toward Highly Sensitive Closed Bipolar Electrochemiluminescence Immunoassay

Chen, Chen; Wang, Yuling; Lin, Xiao; Ma, Shuhui; Cao, Jun‐Tao; Liu, Yan-Ming

doi:10.1021/acsami.3c02381

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…Continuously incorporating new voltage modes and synthesizing highly luminescent ECL probes should be an effective strategy to increase the intensity and stability of ECL. This strategy is being pursued by many research groups [47][48][49][50][51][52][53][54][55][56][57][58][59]. These advancements will further advance single-cell ECL measurement in biological studies and potentially even in clinical tests in the near future.…”

Section: Ecl Measurement Of Biomolecules At the Single-cell Levelmentioning

confidence: 99%

Electrochemiluminescence Detection and Imaging of Biomolecules at the Single-Cell Level

He,

Deng,

Jiang

et al. 2023

Chemosensors

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Electrochemiluminescence (ECL) is an electrochemically induced light produced by the excitation of luminophores in redox reactions. For the past twenty years, ECL analysis has been continuously developed and applied for the sensitive detection of biomolecules at the single-cell level due to its low background interference and the resultant high sensitivity. In recent times, ECL-based microscopy has combined the elements of imaging and has thus emerged as a fast-developed imaging tool to visualize biomolecules in single cells. The surface-confined features of ECL imaging provide detailed information about cell membranes that is not easily obtained using classical fluorescence microscopy. In this review, we summarize the recent works on the detection and imaging of biomolecules at the single-cell level using ECL and discuss the development prospects and challenges in the biological application of this technology in the field of cell analysis.

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Section: Ecl Measurement Of Biomolecules At the Single-cell Levelmentioning

confidence: 99%

Electrochemiluminescence Detection and Imaging of Biomolecules at the Single-Cell Level

He,

Deng,

Jiang

et al. 2023

Chemosensors

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“…The 2D nanosheets of Fe-BTC offered higher diffusion efficiency and more active sites than threedimensional (3D) bulk crystals, which improved the glucose detection performance [41]. Additionally, NiCo-MOF [50,51], Cu-MOF [52][53][54], ZIF-67 [55], and other MOFs were also synthesized using the room-temperature synthesis strategy to detect glucose.…”

Section: Room-temperature Synthesismentioning

confidence: 99%

MOF-Based Materials for Glucose Detection

et al. 2023

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Metal–organic frameworks (MOFs), constructed by coordination between metal-containing nodes and organic linkers, are widely used in various fields due to the advantages of tunable pores, diverse functional sites, stable structure, and multi-functionality. It should be noted that MOF-based materials play a major role in glucose detection, serving as a signal transducer or functional substrate for embedding nanoparticles/enzymes. Diabetes is one of the most common and fast-growing diseases worldwide, whose main clinical manifestation is high blood sugar levels. Therefore, accurate, sensitive, and point-of-care glucose detection is necessary. This review orderly introduces general synthetic strategies of MOF-based materials (pristine MOF, nanoparticles, or enzymes-modified MOF and MOF-derived materials) and detection methods (electrochemical and optical methods) for glucose detection. Then, the review refers to the novel MOF-based glucose detection devices (flexible wearable devices and microfluidic chips), which enable non-invasive continuous glucose monitoring or low-cost microscale detection. On the basis of describing the development of glucose sensors based on MOF materials in the past five years, the review presents merits, demerits, and possible improvements of various detection methods.

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“…Compared with OBPE, CBPE is more suitable for biochemical analysis due to its spatial separation of cathode and anode solutions, making it free from interferences from nonrelated substances. , Furthermore, CBPE requires a lower applied voltage due to the absence of the current shunt . Electrochemiluminescence (ECL) combines the controllability of electrochemistry with the sensitivity of the chemiluminescence method and has been widely used as a powerful analytical technique in various fields. − In recent years, researchers have combined BPE with ECL technology to build BPE-ECL sensors that have become a research hotspot due to their high throughput, low cost, and high sensitivity. − However, most of the BPE-ECL sensors detect the target under single potential, , and ignore the important influence of positive and negative potential co-regulation on BPE. This work is the first time to use dual potential to regulate the polarity of BPE and then innovatively construct a spatial-potential-color simultaneous resolved BPE-ECL sensor.…”

Section: Introductionmentioning

confidence: 99%

Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

Li,

Cai,

Wang

et al. 2024

Anal. Chem.

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A spatial-potential-color-resolved bipolar electrode electrochemiluminescence biosensor (BPE-ECL) using a CuMoOx electrocatalyst was constructed for the simultaneous detection and imaging of tetracycline (TET) and lincomycin (LIN). HOF-101 emitted peacock blue light under positive potential scanning, and CdSe quantum dots (QDs) emitted green light under negative potential scanning. CuMoOx could catalyze the electrochemical reduction of H 2 O 2 to greatly increase the Faradic current of BPE and realize the ECL signal amplification. In channel 1, CuMoOx-Aptamer II (TET) probes were introduced into the BPE hole (left groove A) by the dual aptamer sandwich method of TET. During positive potential scanning, the polarity of BPE (left groove A) was negative, resulting in the electrochemical reduction of H 2 O 2 catalyzed by CuMoOx, and the ECL signal of HOF-101 was enhanced for detecting TET. In channel 2, CuMoOx-Aptamer (LIN) probes were adsorbed on the MXene of the driving electrode (DVE) hole (left groove B) by hydrogen-bonding and metal-chelating interactions. LIN bound with its aptamers, causing CuMoOx to fall off. During negative potential scanning, the polarity of DVE (left groove B) was negative and the Faradic current decreased. The ECL signal of CdSe QDs was reduced for detecting LIN. Furthermore, a portable mobile phone imaging platform was built for the colorimetric (CL) detection of TET and LIN. Thus, the multiple mode-resolved detection of TET and LIN could be realized simultaneously with only one potential scan, which greatly improved detection accuracy and efficiency. This study opened a new technology of BPE-ECL sensor application and is expected to shine in microchips and point-ofcare testing (POCT).

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Cu-MOFs/GOx Bifunctional Probe-Based Synergistic Signal Amplification Strategy: Toward Highly Sensitive Closed Bipolar Electrochemiluminescence Immunoassay

Cited by 8 publications

References 31 publications

Electrochemiluminescence Detection and Imaging of Biomolecules at the Single-Cell Level

Electrochemiluminescence Detection and Imaging of Biomolecules at the Single-Cell Level

MOF-Based Materials for Glucose Detection

Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

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