Culture condition optimization and FT-IR analysis of Polygonum multiflorum Thunb. adventitious root cultures grown in an air-lift bioreactor system

Ho, Thanh-Tam; Le, Kim-Cuong; Kim, Sukweon; Park, Soyoung

doi:10.1007/s11240-020-01961-9

Cited by 14 publications

(5 citation statements)

References 52 publications

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“…Aculeatiside A and B accumulations in the callus of Solanum aculeatissimum were enhanced in solid medium with 0.1 mg/L NAA [ 61 ]. Furthermore, the application of exogenous auxins induced higher total saponins and saponin glycoside contents in the roots of Panax ginseng [ 62 ], Gypsophila paniculata roots [ 24 ], and Bacopa floribunda micropropagated plantlets [ 63 ]. Auxins can also show positive effects on the phenolic production in root cultures.…”

Section: Resultsmentioning

confidence: 99%

“…Auxin has been used as the key regulator of in vitro root induction [ 23 ], which several studies have provided evidence supporting its role in this process. In root induction of Polygonum multiflorum , indole-3-butyric acid (IBA) was more effective than 1-naphthylacetic acid (NAA) in root biomass, phenolic, and flavonoid production [ 24 ], while NAA was suitable for root induction of Gypsophila paniculata and the production of saponins when compared to IBA [ 25 ]. These studies demonstrated that auxin can be used to establish in vitro root culture for phytochemical production, but the appropriate type and concentration of auxin for pokeweeds need to be studied.…”

Section: Introductionmentioning

confidence: 99%

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The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

Trunjaruen,

Luecha,

Taratima

2023

Scientifica

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Pokeweed, Phytolacca americana L., is considered a widely spreading invasive plant, while saponin contents accumulated in the roots have pharmaceutical uses, such as rheumatism treatments and anti-inflammation. Adventitious root cultures are an important source of diverse secondary metabolites, which have significant applications in various fields. This study focused on the optimization of parameters for root induction using different medium states and treatments with auxins on a pokeweed leaf. Semisolid and liquid MS (Murashige and Skoog, 1962) media were supplemented with indole-3-butyric acid (IBA) and 1-naphthylacetic acid (NAA) at 0.5, 1, 2, and 4 mg/L. Root growth parameters, e.g., induction percentage, root numbers, length, and weight, were measured to determine the adventitious root induction efficiency. Total phenolic content, total flavonoid content, total saponin content, and antioxidant activity were recorded. Results showed that adventitious roots induced in semisolid MS medium supplemented with 0.5 mg/L NAA exhibited a high density of lateral roots. Appropriate medium state and auxin for adventitious root induction in pokeweed were determined as semisolid medium supplemented with 2 mg/L NAA. Considering phytochemicals, adventitious roots induced in liquid medium containing 0.5–1 mg/L NAA had the highest yield extract percentage. Additionally, adventitious roots cultivated in a liquid medium enriched with 1 mg/L NAA exhibited the highest phenolic and saponin contents. A principal component analysis (PCA) biplot and hierarchical cluster analysis (HCA) heatmap demonstrated different response patterns between semisolid and liquid media applied with NAA. The results of the semisolid media were grouped together due to high expression levels of the root induction parameters, while elevated phytochemical values were observed in the liquid media treatments. The results suggested two different media that provide the highest adventitious root induction efficiency and the greatest phytochemical contents: semisolid medium with 2 mg/L NAA and liquid medium with 1 mg/L NAA, respectively. These culture media can be applied to optimize adventitious root culture of pokeweed and in vitro phytochemical production.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

Trunjaruen,

Luecha,

Taratima

2023

Scientifica

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“…The interest in plant cell and organ cultures has increased due to plant biotechnology, as many of the scale up studies for the production of promising secondary metabolic products were successful (Pandey et al, 2019;Szopa et al, 2019;Ho et al, 2020;Kokotkiewicz et al, 2021). Elicitation strategies for the enhancement of bioactive components in edible plant sprouts has been reviewed in detail by Toro et al (2021) and their benefits have been reported.…”

Section: O N L I N E C O P Ymentioning

confidence: 99%

Elicitation based enhancement of solasodine production in in-vitro cultures of different Solanum species

Deshmukh¹,

Deshpande²,

Wani³

2023

JEB

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Aim: To study the enhancement of solasodine content using elicitors such as NaCl, pectin, salicylic acid and yeast extract in cell suspension cultures of Solanum incanum, Solanum nigrum, Solanum surattense and Solanum villosum. Methodology: In-vitro callus induction from leaf explants was carried out on MS media supplemented with auxin, 2, 4- Dichlorophenoxyacetic acid (2.0 mg l-1). MS liquid medium supplemented with 2, 4-D (2.0 mg l-1) and varied concentrations of different elicitors were used for cell suspension culture. Results: The elicitor NaCl (150 mM) indicated maximum increment in solasodine production in three Solanum species studied. Salicylic acid with 75 μM resulted in considerable elevation in solasodine content in Solanum spp. Response to elicitation by pectin was high at different concentrations for different species of Solanum. Biotic elicitor yeast extract at 3.0 g l-1 concentration considerably increased solasodine production in S. nigrum and S. villosum. Solanum villosum exhibited best results in terms of solasodine concentration enhancement in response to pectin and yeast extract elicitors whereas Solanum incanum responded best to pectin followed by NaCl. Interpretation: The current results indicated that NaCl, pectin, salicylic acid and yeast extract can be effectively applied as influential elicitors for the enhancing the production of solasodine in cell suspension culture of different Solanum species. Key words: Callus, Cell suspension culture, Elicitors, Solanum, Solasodine, Steroidal alkaloids

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“…In a 500 L pilot-scale bioreactor, an optimal yield of 4.01 kg dry root biomass and 287.12 mg/L total phenolic productivity of Polygonum multiflorum was attained [ 86 ]. In the study, [ 87 ] investigated the effects of several auxins on Polygonum multiflorum Thunb . Adventitious root cultures in a 3 L balloon-type bubble bioreactor (BTBB).…”

Section: Case Studiesmentioning

confidence: 99%

“…Ref. [ 87 ] reported that indole-3-butyric acid increased the production of hairy roots in E. purpurea by 14-folds. To improve transformation, other inducers of Rhizobium-associated gene transfer in plants such as 6-benzylaminopurine, 2,4-dichloro phenoxyacetic acid have also been applied to E. purpurea hairy root cultures [ 116 ].…”

Section: Case Studiesmentioning

confidence: 99%

Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review

Hussain

Abbas

Nazli

et al. 2022

Plants

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Medicinal plants are an inevitable source of pharmaceutical drugs and most of the world population depends on these plants for health benefits. The increasing global demand for bioactive compounds from medicinal plants has posed a great threat to their existence due to overexploitation. Adventitious root and hairy root culture systems are an alternative approach to the conventional method for mass production of valuable compounds from medicinal plants owing to their rapid growth, biosynthetic and genetic stability. The main purpose of this review is to investigate the recent scientific research published worldwide on the application of adventitious and hairy root cultures to produce valuable compounds from medicinal plants. Furthermore, a comparison of adventitious root vs. hairy root cultures to produce valuable compounds has also been discussed. Various aspects such as medium composition, carbon source, pH, amount of macronutrients, optimization strategy, scale-up cultures, and use of biotic abiotic and nano-elicitors at various concentrations are the topic of discussion in this review. Several studies on adventitious and hairy root cultures of Polygonum multiflorum¸ Withania somnifera¸ Echinacea purpurea and Ajuga bracteosa have been discussed in detail which highlights the importance of elicitation strategies and bioreactor system, presenting commercial applications.

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Culture condition optimization and FT-IR analysis of Polygonum multiflorum Thunb. adventitious root cultures grown in an air-lift bioreactor system

Cited by 14 publications

References 52 publications

The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

Elicitation based enhancement of solasodine production in in-vitro cultures of different Solanum species

Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review

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