Culture‐expanded mesenchymal stem cell sheets enhance extraction‐site alveolar bone growth: An animal study

Mu, Sen; Tee, Boon Ching; Emam, Hany A.; Zhou, Yitong; Sun, Zheng

doi:10.1111/jre.12541

Cited by 14 publications

(19 citation statements)

References 48 publications

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“…15,39 All these characteristics have made Al-BMSCs potentially more desirable for wide therapeutic applications. Although it has been found that Al-BMSCs sheet would increase local bone density when transplanted into the tooth-extraction site, 16 the function of Al-BMSCs sheet in vitro and the craniofacial bone defect repairment features in vivo remain unclear. Our results showed that Al-BMSCs sheet have the similar histology structure, higher osteogenic differentiation capacity and bone defect reconstruction in rabbit calvarial defect model, demonstrating Al-BMSCs sheet based tissue regeneration would be a promising strategy for craniofacial defect repair in future clinical applications.…”

Section: Discussionmentioning

confidence: 99%

“…15 It has also been found that Al-BMSCs can be induced to cell sheets, and can increase local bone density when transplanted into the tooth-extraction site. 16 However, although several studies have compared the character of Al-BMSCs and BMSCs in vitro and in vivo , most studies are in animal models, only a few articles are relevant to human study. In addition, the difference of Al-BMSCs sheet and BMSCs sheet in vitro and the craniofacial bone defect repairment features in vivo remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect

et al. 2020

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Mesenchymal stem cells sheets have been verified as a promising non-scaffold strategy for bone regeneration. Alveolar bone marrow mesenchymal stem cells, derived from neural crest, have the character of easily obtained and strong multi-differential potential. However, the bone regenerative features of alveolar bone marrow mesenchymal stem cells sheets in the craniofacial region remain unclear. The purpose of the present study was to compare the osteogenic differentiation and bone defect repairment characteristics of bone marrow mesenchymal stem cells sheets derived from alveolar bone (alveolar bone marrow mesenchymal stem cells) and iliac bone (Lon-bone marrow mesenchymal stem cells) in vitro and in vivo. Histology character, osteogenic differentiation, and osteogenic gene expression of human alveolar bone marrow mesenchymal stem cells and Lon-bone marrow mesenchymal stem cells were compared in vitro. The cell sheets were implanted in rabbit calvarial defects to evaluate tissue regeneration characteristics. Integrated bioinformatics analysis was used to reveal the specific gene and pathways expression profile of alveolar bone marrow mesenchymal stem cells. Our results showed that alveolar bone marrow mesenchymal stem cells had higher osteogenic differentiation than Lon-bone marrow mesenchymal stem cells. Although no obvious differences were found in the histological structure, fibronectin and integrin β1 expression between them, alveolar-bone marrow mesenchymal stem cells sheet exhibited higher mineral deposition and expression levels of osteogenic marker genes. After being transplanted in the rabbit calvarial defects area, the results showed that greater bone volume and trabecular thickness regeneration were found in bone marrow mesenchymal stem cells sheet group compared to Lon-bone marrow mesenchymal stem cells group at both 4 weeks and 8 weeks. Finally, datasets of bone marrow mesenchymal stem cells versus Lon-bone marrow mesenchymal stem cells, and periodontal ligament mesenchymal stem cells (another neural crest derived mesenchymal stem cells) versus umbilical cord mesenchymal stem cells were analyzed. Total 71 differential genes were identified by overlap between the 2 datasets. Homeobox genes, such as LHX8, MKX, PAX9, MSX, and HOX, were identified as the most significantly changed and would be potential specific genes in neural crest mesenchymal stem cells. In conclusion, the Al-bone marrow mesenchymal stem cells sheet-based tissue regeneration appears to be a promising strategy for craniofacial defect repair in future clinical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect

et al. 2020

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“…Considering the regenerated tissues, 24 articles focused on bone , 17 on periodontium 49-65 , 10 on dentin pulp-complex 66-75 and 7 on tooth or tooth root [76][77][78][79][80][81][82] . Regarding bone regeneration, 8 studies developed a porcine model [25][26][27][28][29][30][31][32] , 14 a canine [33][34][35][36][37][38][39][40][41][42][43][44][45][46] and 2 an ovine one 47,48 . Eight studies reported periodontium regeneration in pig [49][50][51][52][53][54][55][56] , 8 in dog [57][58][59][60][61][62][63][64] and 1 in sheep 65 .…”

Section: Characteristics Of the Included Studiesmentioning

confidence: 99%

Characteristics of Large Animal Models for Current Cell-Based Oral Tissue Regeneration

Mangione

Salmon

EzEldeen

et al. 2022

Tissue Engineering Part B: Reviews

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The recent advances in the field of cell-based therapeutics open promising perspectives for oral tissue regeneration. The development of large animal models, which overcome the limits of the rodent models and allow to emulate clinical situations, is crucial for the validation of regenerative strategies to move towards clinical application.Currently, porcine, canine and ovine models are the mainly developed for oral regeneration and their specific characteristics have an impact on the outcomes of the studies.Thus, this systematic review investigates the application of porcine, canine and ovine models in present cell-based oral regeneration, according to the species characteristics and the targeted tissue to regenerate.A customized search of PubMed, EMBASE, Scopus, and Web of Science databases, from January 2015 to March 2020 was conducted. Relevant articles about cell-based oral tissues engineering in porcine, canine and ovine models were evaluated. Among the evaluated articles, fifty-eight relevant studies about cell-based oral regeneration in porcine, canine and ovine models matched the eligibility criteria and were selected for full analysis.Porcine models, the most similar specie with humans, were mostly used for bone and periodontium regeneration; tooth regeneration was reported only in pig except for one study in dog.Canine models were the most transversal models, successfully involved for all oral tissues regeneration and notably in implantology. However, differences with humans and ethical concerns affect the use of these models.Ovine models, alternative to porcine and canine ones were mainly used for bone and, scarcely, for periodontium regeneration. The anatomy and physiology of these animals restrain their involvement.

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“…As control, bone without treatment was taken. The side with the cell sheet was thicker and the bone volume was larger [84].…”

Section: Stem Cells-based Scaffold-free Bone Regenerationmentioning

confidence: 99%

The Potential of Different Origin Stem Cells in Modulating Oral Bone Regeneration Processes

Buduru

Gulei

Zimța

et al. 2019

Cells

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Tissue engineering has gained much momentum since the implementation of stem cell isolation and manipulation for regenerative purposes. Despite significant technical improvements, researchers still have to decide which strategy (which type of stem cell) is the most suitable for their specific purpose. Therefore, this short review discusses the advantages and disadvantages of the three main categories of stem cells: embryonic stem cells, mesenchymal stem cells and induced pluripotent stem cells in the context of bone regeneration for dentistry-associated conditions. Importantly, when deciding upon the right strategy, the selection needs to be made in concordance with the morbidity and the life-threatening level of the condition in discussion. Therefore, even when a specific type of stem cell holds several advantages over others, their availability, invasiveness of the collection method and ethical standards become deciding parameters.

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Culture‐expanded mesenchymal stem cell sheets enhance extraction‐site alveolar bone growth: An animal study

Abstract: Mandibular marrow-derived MSCs can be fabricated into cell sheets and autogenous transplantation of MSC sheets onto the subperiosteal buccal alveolar bone surface at the tooth-extraction site may increase local bone density.

Cited by 14 publications

References 48 publications

Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect

Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect

Characteristics of Large Animal Models for Current Cell-Based Oral Tissue Regeneration

The Potential of Different Origin Stem Cells in Modulating Oral Bone Regeneration Processes

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