Culture medium optimization for camptothecin production in cell suspension cultures of Nothapodytes nimmoniana (J. Grah.) Mabberley

Karwasara, Vijai Singh; Dixit, Vinod Kumar

doi:10.1007/s11816-012-0270-z

Cited by 70 publications

(31 citation statements)

References 58 publications

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“…However, yield of CPT in the callus cultures were reduced to about half the productivity obtained on solid medium cultivation system but, overall productivity relatively higher (Fig. 2B-F), despite the possibility of degradation of the leached CPT in liquid medium cultivation system [20,71]. For evaluation of the influence of PGRs on biomass production and biosynthesis of CPT in C. fragrans callus cultures, absolute biomass production in callus obtained from in vitro morphogenesis study and CPT production were evaluated by assessment of fresh and dried weight, alkaloid extracted and HPTLC analysis performed [21].…”

Section: Influence Of Pgrs On Production Of Camptothecin In Seed Embrmentioning

confidence: 94%

“…Purine-type of the cytokinins have beneficial effect on alkaloids production in the in vitro cultures without adverse effect on callus growth while phenylurea derivatives either inhibit growth of cultures or highly improve biomass production at the expense of alkaloids biosynthesis [41]. Therefore, modification of their combination and ratio is an effective strategy for studying plant secondary metabolites biosynthetic capacity of cultures during in vitro morphogenesis [41,71]. For instance, in the tender-stem induced callus cultures of Securinega suffruticosa, production of biomass and virosecurinine alkaloids was influenced by sucrose concentration and PGRs amended in the cultivation medium, and combination of Kin with NAA was the best for the productivity [72].…”

Section: Influence Of Pgrs On Production Of Camptothecin In Seed Embrmentioning

confidence: 99%

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Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

Isah

2019

Preprint

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Background: In vitro morphogenic response of mature seed embryo-derived callus cultures of Chonemorpha fragrans was studied using solid and liquid Murashige and Skoog medium amended with cytokinins or their combinations with naphthalene acetic acid at 0.5 mg L-1.Results: The tested plant growth regulators combination and concentrations could not stimulate organogenesis after three subcultivations of the callus cultures on the same PGRs-amended solid medium, and when cultivated in the liquid but, formation of morphogenic callus was observed. Evaluation of biomass and camptothecin production showed that the PGRs influenced biomass and CPT yield of the callus cultures. The alkaloid yield of various explants of 3–4 weeks old axenic seedlings was higher in roots (0.019 % CPT) followed by mature seed embryos (0.0053 %), cotyledons (0.0039 %), hypocotyls (0.0024 %) and leaves (0.0017 %). There was no significant difference in yield of CPT from callus induced from the various explants. Camptothecin yield of morphogenic callus cultures cultivated in liquid medium was lower than that of solid due to extracellular leaching effect of the alkaloid. Amount of synthesized CPT in the callus cultures also varied with PGR type and concentration amended in the cultivation medium, and showed association with biomass production.Conclusion: Results of the present study suggest that callus cultures offer alternative tissue source for in vitro CPT yield enhancement through biotechnological approaches, with application in the large-scale production of the alkaloid to conserve the ever decimated natural population of the medicinal woody climber for CPT.

show abstract

Section: Influence Of Pgrs On Production Of Camptothecin In Seed Embrmentioning

confidence: 94%

Section: Influence Of Pgrs On Production Of Camptothecin In Seed Embrmentioning

confidence: 99%

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

Isah

2019

Preprint

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show abstract

“…However, yield of CPT in the callus cultures were reduced to about half the productivity obtained on solid medium cultivation system but, overall productivity relatively higher (Fig. 2B-F), despite the possibility of degradation of the leached CPT in liquid medium cultures [19,71]. For evaluation of the influence of PGRs on biomass production and biosynthesis of CPT in the C. fragrans callus cultures, absolute biomass production in callus obtained from in vitro morphogenesis study and CPT production were evaluated by the measured fresh and dried weight (after dried to constant mass), the alkaloid extracted and HPTLC analysis performed.…”

Section: Influence Of Pgrs On Production Of Camptothecin In Seed Embrmentioning

confidence: 94%

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

Isah¹,

Umar²

2019

Preprint

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Background: In vitro morphogenic response of mature seed embryo-induced callus cultures of Chonemorpha fragrans was studied using solid and liquid Murashige and Skoog medium amended with cytokinins or their combinations with naphthalene acetic acid at 0.5 mg L-1. Results: The tested plant growth regulators combination and concentrations did not stimulate organogenesis after three subcultivations of the callus cultures on the same PGRs-amended solid medium, and when cultivated in the liquid but, formation of morphogenic callus was observed. Evaluation of biomass and camptothecin production showed that the PGRs influenced biomass and CPT yield of the callus cultures. The alkaloid yield of various explants of 3–4 weeks old axenic seedlings was higher in roots (0.019 % CPT) followed by mature seed embryos (0.0053 %), cotyledons (0.0039 %), hypocotyls (0.0024 %) and leaves (0.0017 %). There was no significant difference in the yield of CPT from callus induced from the various explants. Camptothecin yield of morphogenic callus cultures cultivated in liquid medium was lower than that of solid due to extracellular leaching effect of the alkaloid. Amount of synthesized CPT in the callus cultures also varied with the PGR type and concentration amended in the cultivation medium, and showed association with biomass production. Conclusion: Results of the present study suggest that callus cultures offer alternative tissue source for the in vitro CPT yield enhancement through biotechnological approaches, with application in the large-scale production of the alkaloid to conserve the ever decimated natural population of this medicinal woody climber for the alkaloid.

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“…Callus induction was observed within 10 days of inoculation of explants, then the CPT content was analyzed using HPLC and found to be 1.306% of DW in MS medium supplemented with 2, 4-D and BAP. Karwasara et al [38] studied the effect of culture media nutrients on the growth and production of camptothecin by varying sugar, nitrate and ammonium concentrations. Modified MS medium was prepared which contained 0.5 mM phosphate, a nitrogen source feeding ratio of 50/10 mM NH 4 + /NO 3 -and 3 % sucrose with additional 2 % sucrose feeding (added on day 12 of the cell culture cycle) with 10.74 µM NAA and 0.93 µM KN.…”

Section: Post Strictosidine Part (Cpt Biosynthesis)-mentioning

confidence: 99%

Production of Camptothecin from Nothapodytes nimmoniana: An Overview

Pr¹,

Xavier²,

Reddy³

et al. 2017

IJLSSR

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ABSTRACT-Nothapodytes nimmoniana is an endangered tree endemic to Western Ghats, India and it is the convenient source for large scale isolation of camptothecin (CPT). Since the first report of CPT detection in N. nimmoniana, significant work has been done on its applications. Due to heavy collection of its wood chips for CPT, population is under threat in India. Several plant tissue culture techniques offer alternative strategies for clonal propagation and CPT production to conserve the species. Various strategies are employed to enhance in vitro condition response through culture medium optimization, elicitation, and artificial seed method. In this article, we have reviewed progress made so far on different methods of plant tissue culture techniques for production of camptothecin from N. nimmoniana and biosynthesis of CPT in details.

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Culture medium optimization for camptothecin production in cell suspension cultures of Nothapodytes nimmoniana (J. Grah.) Mabberley

Cited by 70 publications

References 58 publications

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

Production of Camptothecin from Nothapodytes nimmoniana: An Overview

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