Culture medium optimization for enhanced β-carotene and biomass production by Dunaliella salina in mixotrophic culture

Morowvat, Mohammad Hossein; Ghasemi, Younes

doi:10.1016/j.bcab.2016.06.008

Cited by 86 publications

(37 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…UV/vis spectrophotometry method at 450 nm absorbance was used for detection of β-carotene content. 22,25 Total lipid content was extracted and quantified using gravimetric method, 5 using a mixture of methanol/ dichloromethane (2:1, v/v). 22 The lipid extraction procedure was repeated for three times.…”

Section: Methodsmentioning

confidence: 99%

“…22,25 Total lipid content was extracted and quantified using gravimetric method, 5 using a mixture of methanol/ dichloromethane (2:1, v/v). 22 The lipid extraction procedure was repeated for three times. Fatty acid composition analysis was conducted as described before.…”

Section: Methodsmentioning

confidence: 99%

“…The isolated unialgal culture was gained by sub culturing the alga in a fresh Johnson medium (pH 7.5) with 12% salinity. 22 Moreover, the microscopic analysis and identification of the cells were done on colonies grown on solid Johnson medium using previously described methods. 23 The ribosomal 18S rRNA gene sequencing using PCR method was accomplished using single cell taken directly from plates.…”

Section: Algal Source and Culture Conditionsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Shaker¹,

Morowvat²,

Ghasemi³

2017

JYP

Self Cite

View full text Add to dashboard Cite

Objective: Microalgal pharmaceutical biotechnology is mainly dependent on the biomass yield and also the final concentration of the obtained lipids. β-carotene is one of the most precious nutraceuticals, of both preventive and therapeutics importance in pharmacy and medicine. Dunaliella salina is known as famous β-carotene producer which could accumulate the β-carotene up to 10% of its dry cell weights. The amount of different macro and micronutrients in D. salina culture medium defines its productivity and β-carotene content. Methods: In this study, the effects of sulfur, iron and manganese deprivation, on cell growth and β-carotene biosynthesis in a naturally isolated strain of D. salina was examined. Besides, the fatty acid profile of the naturally isolated strain was also investigated. Results: Sulfur, iron and manganese deprivation caused a noticeable decrease in the cell growth of D. salina. On the other hand, in nutrient depleted media, the maximum β-carotene concentration was significantly improved (14.616 mg L-1 in sulfur starvation, 14.994 mg L-1 in iron starvation and 10.119 mg L-1 in manganese starvation media) compared with initial values (6.753 mg L-1) in basic culture medium. The obtained fatty acids from the studied microalgal strain found to be some important saturated, monounsaturated and polyunsaturated fatty acids. Conclusion: Owing to its significant growth rate, β-carotene contents and fatty acid profile; the naturally isolated microalgal strain could be exploited as a potential producer strain. Besides, the nutrient limitation strategy could be effectively employed to improve the β-carotene production procedure in D. salina. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Algal Source and Culture Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Shaker¹,

Morowvat²,

Ghasemi³

2017

JYP

Self Cite

View full text Add to dashboard Cite

show abstract

“…[18] The lyophilized biomass of C. vulgaris strain was used for this analysis. Pure olive oil was used to plot the standard curve for lipid determination.…”

Section: Methodsmentioning

confidence: 99%

Investigation of autotrophic, heterotrophic, and mixotrophic modes of cultivation on lipid and biomass production in Chlorella vulgaris

Sajadian¹,

Morowvat²,

Ghasemi³

2018

Natl J Physiol Pharm Pharmacol

Self Cite

View full text Add to dashboard Cite

Background:The cultivation mode plays a great role in biomass production and composition in the green microalga Chlorella vulgaris. Aims and Objectives: The effects of three different cultivation modes including autotrophic, heterotrophic, and mixotrophic growth conditions on the biomass and lipid production and also on the biomass composition in the unicellular photosynthetic microalga C. vulgaris were investigated. Materials and Methods: BG-11 culture medium was used for preservation and analysis of the studied microalgal strain. Effects of three different cultivation strategies including autotrophic, heterotrophic, and mixotrophic modes of cultivation were investigated on biomass and lipid production in C. vulgaris. Results: The maximum amount of cell dry weight was obtained in mixotrophic mode (3.91 g L

show abstract

“…Based upon it, the medium composition plays a great role in microalgal growth (Morowvat & Ghasemi, 2016), and subsequently affects carotenoids production. In this present study, the starvation strategy is more effective in Johnson than f/2 medium.…”

Section: Response To Nitrate Starvation During Stress Phasementioning

confidence: 99%

Untitled

Achour¹,

Doumandji²,

Bouras³

et al. 2019

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Algeria has a high algal biodiversity, which is of great interest and should be exploited. Isolation of Dunaliellastrain DunaDz1 from SidiAmeur Lake in Algeria was carried out in this study. The aim of this research was to identify the isolated strain by morphological and molecular taxonomy using ITS and rbcL genes. The effect of two stress factors, salinity and nitrate starvation on the carotenoids accumulation in Dunaliella strain was investigated on f/2 and Johnson media. The results for both genes showed that strain DunaDz1 is part of a clade containing several strains of D. salina. The strain grew well in Johnson medium, with a cell number of 56.09 10 5 cell/ml and a chlorophyll a amount of 63.63 µg/ml. Salt stress lead to a noticeable increase in the total carotenoids levels of strain DunaDz1, 23.68 ± 0.12 and 23.15 ± 0.23 pg/cell, for f/2 and Johnson media, respectively. In nitrate starved media a lesser amount was recorded comparing with salt stress: 11.42 ± 0.22 and 16.2 ± 0.24 pg/cell, for f/2 and Johnson media, respectively. The combination of these two factors was more effective on f/2 medium than Johnson medium.

show abstract

Culture medium optimization for enhanced β-carotene and biomass production by Dunaliella salina in mixotrophic culture

Cited by 86 publications

References 28 publications

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Investigation of autotrophic, heterotrophic, and mixotrophic modes of cultivation on lipid and biomass production in Chlorella vulgaris

Untitled

Contact Info

Product

Resources

About