Culture of neural cells on polymers coated surfaces for biosensor applications

Lakard, Sophie; Herlem, Guillaume; Valles-Villareal, N.; Michel, Germaine; Propper, Alain; Gharbi, Tijani; Fahys, Bernard

doi:10.1016/j.bios.2004.09.001

Cited by 51 publications

(21 citation statements)

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“…They assigned this limited cell growth to surface tension and irregular roughness in the oxidized films, with the possibility that tosylate diffusion into the culture media worsened the outcome on overoxidized films. Poor proliferation was also reported by Lakard et al (2004Lakard et al ( , 2005 for a rat neuronal cell line on PPy when compared to other substrates including those that were electrodeposited (figure 6). Furthermore, Castano et al (2004) showed a dependence on film thickness as controlled by monomer concentration during admicellar polymerization, where a surfactant, monomer and initiator are used to form the polymer, for the viability and differentiation of mesenchymal stem cells towards the osteoblastic phenotype.…”

Section: Mattioli-belmonte Et Al (2003) Studied the Tissuementioning

confidence: 94%

Polypyrrole-based conducting polymers and interactions with biological tissues

Ateh

Navsaria

Vadgama

2006

J. R. Soc. Interface.

459

260

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Polypyrrole (PPy) is a conjugated polymer that displays particular electronic properties including conductivity. In biomedical applications, it is usually electrochemically generated with the incorporation of any anionic species including also negatively charged biological macromolecules such as proteins and polysaccharides to give composite materials. In biomedical research, it has mainly been assessed for its role as a reporting interface in biosensors. However, there is an increasing literature on the application of PPy as a potentially electrically addressable tissue/cell support substrate. Here, we review studies that have considered such PPy based conducting polymers in direct contact with biological tissues and conclude that due to its versatile functional properties, it could contribute to a new generation of biomaterials.

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Section: Mattioli-belmonte Et Al (2003) Studied the Tissuementioning

confidence: 94%

Polypyrrole-based conducting polymers and interactions with biological tissues

Ateh

Navsaria

Vadgama

2006

J. R. Soc. Interface.

459

260

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“…66 In addition, electrodeposited PEI films were superior in the growth of olfactory cells, as compared with other positively charged biopolymers. 60 As shown in Figures 5-7, an increase in the concentration of PEI from 210 to 300 lg/mL in the surface-modified scaffolds exhibited few improvements in the culture of BKCs. This indicated that the optimal upper limit of the concentration of PEI could be about 210 lg/mL in the case of surface modification.…”

Section: Culture Of Constructsmentioning

confidence: 95%

“…Briefly, one construct was digested by 95.24 lg of papain in 2 mL of Tris buffer, containing 55 mM trisodium citrate, 150 mM NaCl, 5 mM cysteine, and 5 mM EDTA at 60 C over 24 h. The amount of BKCs was determined by Hoechst No. 33258 with a fluorescence spectrophotometer (F-4500, Hitachi, Tokyo, Japan) at 365 nm excitation wavelength and 458 nm emission wavelength.…”

Section: Cultivation Of Constructs and Biochemical Analysismentioning

confidence: 99%

“…59 Besides amine groups, hydrophobic methyl groups in PEI could also promote the adhesion of BKCs. 60 For a fixed concentration of PEI, the adhesion efficiency of a surfacemodified scaffold was higher than that of a bulk-modified scaffold, as indicated in Figure 4. The rationale behind this result was that bulk modification in a scaffold distributed PEI throughout the solid phase, yielding a reduced concentration of PEI on the pore surfaces.…”

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confidence: 91%

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Application of polyethyleneimine‐modified scaffolds to the regeneration of cartilaginous tissue

Kuo

2009

Biotechnology Progress

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In this study, we analyzed the physicochemical and biophysical properties of three-dimensional scaffolds modified using polyethyleneimine (PEI) and applied these scaffolds to the cultivation of bovine knee chondrocytes (BKCs). PEI was crosslinked in the bulk or on the surface of the ternary scaffolds comprising polyethylene oxide, chitin and chitosan. The results revealed that when the concentration of PEI was less than 300 microg/mL, the cytotoxicity of a scaffold was on the same order in the two method of modification. An increase in the concentration of PEI favored the adhesion of BKCs. When the amount of PEI in scaffolds is fixed, the surface-modified scaffolds exhibited a higher adhesion efficiency of BKCs than the bulk-modified scaffolds. For the regeneration of cartilaginous components, a higher amount of PEI in a scaffold yielded larger amounts of proliferated BKCs, secreted glycosaminoglycans, and produced collagen. In addition, the formation of neocartilage in the surface-modified scaffolds was more effective than that in the bulk-modified scaffolds. These tissue-engineered scaffolds, modified by an appropriate concentration of PEI, can be potentially applied to cartilage repair in clinical trials.

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“…4 Factors, such as the composition, hydrophobicity, and topography of the surface, electrical charges, materials viscosity, and protein interactions play an important role in the cell proliferation on alloplastic materials. [5][6][7] Ideally, directly influencing the biocompatibility via fabrication of an appropriate "design" of the surface should be possible. This might also have a beneficial effect on negative long-term side effects which may to a certain extent result from the mesh, such as pain syndromes.…”

Section: Introductionmentioning

confidence: 99%

In vitroanalysis of biopolymer coating with glycidoxypropyltrimethoxysilane on hernia meshes

Metzler

Zankovych

Rauchfuß

et al. 2016

J. Biomed. Mater. Res.

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Certain coatings may improve the biocompatibility of hernia meshes. The coating with self-assembled monolayers, such as glycidoxypropyltrimethoxysilane (GOPS) can also improve the materials characteristics of implants. This approach was not yet explored in hernia meshes. It was the aim of this work to clarify if and how hernia meshes with their three-dimensional structure can be coated with GOPS and with which technique this coating can be best characterized. Commercially available meshes made from polypropylene (PP), polyester (PE), and expanded polytetrafluorethylene (ePTFE) have been coated with GOPS. The coatings were analyzed via X-ray photoelectron spectroscopy (XPS), confocal laser scanning microscopy (CLSM), and cell proliferation test (mouse fibroblasts). Cell viability and cytotoxicity were tested by MTT test. With the GOPS surface modification, the adherence of mouse fibroblasts on polyester meshes and the proliferation on ePTFE meshes were increased compared to noncoated meshes. Both XPS and CLSM are limited in their applicability and validity due to the three-dimensional mesh structure while CLSM was overall more suitable. In the MTT test, no negative effects of the GOPS coating on the cells were detected after 24 h. The present results show that GOPS coating of hernia meshes is feasible and effective. GOPS coating can be achieved in a fast and cost-efficient way. Further investigations are necessary with respect to coating quality and adverse effects before such a coating may be used in the clinical routine. In conclusion, GOPS is a promising material that warrants further research as coating of medical implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1083-1090, 2017.

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Culture of neural cells on polymers coated surfaces for biosensor applications

Cited by 51 publications

References 50 publications

Polypyrrole-based conducting polymers and interactions with biological tissues

Polypyrrole-based conducting polymers and interactions with biological tissues

Application of polyethyleneimine‐modified scaffolds to the regeneration of cartilaginous tissue

In vitroanalysis of biopolymer coating with glycidoxypropyltrimethoxysilane on hernia meshes

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