Cultured cambial meristematic cells as a source of plant natural products

Lee, Eun-Kyong; Jin, Young-Woo; Park, Joong Hyun; Yoo, Young Mi; Hong, Sun Mi; Amir, Rabia; Yan, Zejun; Kwon, Eunjung; Elfick, Alistair; Tomlinson, Simon R.; Halbritter, Florian; Waibel, Thomas; Yun, Byung‐Wook; Loake, Gary J.

doi:10.1038/nbt.1693

Cited by 164 publications

(165 citation statements)

References 42 publications

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“…The OrthoMCL algorithm was applied to generate orthologous groups for the transcriptome datasets of 19 non-model plants, P. cuspidatum (this study), Fagopyrum esculentum, F. tataricum [15], T. mairei [11], Korea T. cuspidata [24], China T. cuspidata [25], Ginkgo biloba [26], Huperzia serrata, Phlegmariurus carinatus [27], Pteridium aquilinum [28], Panax quinquefolius [29], Panax ginseng [30], Salvia miltiorrhiza [31], Camptotheca acuminata [32], Artemisia annua [33], Cucurbita pepo [34], Glycyrrhiza uralensis [35], Eucalyptus hybrid [36], and Oryza longistaminata [37]. These plants cover a broad range of ferns, gymnosperms, monocots, and eudicots.…”

Section: Orthologous Clusteringmentioning

confidence: 99%

De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatum

Hao

Ma²,

et al. 2012

Sci. China Life Sci.

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Various active components have been extracted from the root of Polygonum cuspidatum. However, the genetic basis for their activity is virtually unknown. In this study, 25600002 short reads (2.3 Gb) of P. cuspidatum root transcriptome were obtained via Illumina HiSeq 2000 sequencing. A total of 86418 unigenes were assembled de novo and annotated. Twelve, 18, 60 and 54 unigenes were respectively mapped to the mevalonic acid (MVA), methyl-D-erythritol 4-phosphate (MEP), shikimate and resveratrol biosynthesis pathways, suggesting that they are involved in the biosynthesis of pharmaceutically important anthraquinone and resveratrol. Eighteen potential UDP-glycosyltransferase unigenes were identified as the candidates most likely to be involved in the biosynthesis of glycosides of secondary metabolites. Identification of relevant genes could be important in eventually increasing the yields of the medicinally useful constituents of the P. cuspidatum root. From the previously published transcriptome data of 19 non-model plant taxa, 1127 shared orthologs were identified and characterized. This information will be very useful for future functional, phylogenetic and evolutionary studies of these plants.

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Section: Orthologous Clusteringmentioning

confidence: 99%

De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatum

Hao

Ma²,

et al. 2012

Sci. China Life Sci.

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“…At present, most of the strategies rely on the selection of a specifi c type of plant cells which possess a defi ned differentiation state that appears to be optimal for the production of a particular bioactive compound. This strategy is perfectly illustrated by the use of innately undifferentiated cambial meristematic cells (CMCs) from Taxus cuspidata , for the production of paclitaxel (Taxol), an economically important anticancer drug [ 76 ]. The attributes of this culture system are that the cambial meristematic cells bypass the negative effects of the dedifferentiation step, therefore, their activity for producing paclitaxel is stable for long periods under a low auxin regime (i.e.…”

Section: Manipulation Of Mechanisms Governing Cell Differentiation Inmentioning

confidence: 99%

Theoretical Basis of Plant Cell and Tissue Culture for Production of Biomass and Bioactive Compounds

López‐Villalobos

Yeung

Thorpe

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant tissue culture is an important biotechnological tool, which involves biochemical and genetic manipulations to onset specifi c gene programming, which determines an optimal cell differentiation state for the production of bioactive compounds. Indeed, not only the metabolism but also the morphogenetic processes are modifi ed in plant cells to drive the synthesis and accumulation of economically valuable compounds. In this chapter, we provide an overview of the current molecular approaches to control the expression of specifi c genes encoding putative heterologous and native enzymes as well as transcription factors to enhance the metabolic fl ow of specifi c pathways in order that notable bioactive compounds can be accumulated in plant cells at acceptable commercial levels. Such methods vary from singlegene plant transformations to the emerging multi-gene transformation (gene stacking) technologies embraced by private companies focusing primarily in the metabolic engineering of secondary metabolism. The virtues and potential of these molecular methods in their application to tissue culture systems are presented. Additionally, the importance of subcellular targeting of proteins, notably biosynthetic enzymes and pharmaceutical antibodies, for enhancing their activity and stability is also discussed. Finally, the progress in two emerging approaches for the production of bioactive molecules, the manipulation of cell differentiation and cell immortalization are expounded in this article. Thus, we present the molecular basis to control both cell differentiation and cell immortalization in plant tissue culture systems as novel avenues to control and perpetuate the gene programming which in turn creates and regulates cellular microenvironments for the optimal biosynthesis of valuable compounds. Consequently, our objective is to present how basic approaches, including the manipulation of gene expression, are amalgamated to other molecular strategies of higher hierarchy, particularly the manipulation of cell differentiation and immortalization for the synthesis of bioactive molecules in plant tissue culture platforms.

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“…However, the working concentrations of neutral red vary depending on the tissue types and plant species under investigation. For example, low concentrations are used to stain Arabidopsis cell suspensions, 0.1 mg/L [27,28], Brassica protoplasts, 1 mg/L [29], and Arabidopsis roots, 1 mg/L [30], whereas high concentrations are required for onion parenchyma cells, 4 mg/L [31] and whole carrots seedlings and embryos, 20 mg/L [25].…”

Section: Vacuolementioning

confidence: 99%