“…Using optical, SEM and confocal microscopy, we showed the presence of two different cell subpopulations, differing in nucleus size. The three-dimensional organization of the aggregates suggests the existence of some kind of functional compartmentalization and that they are similar to human embryonic stem cell aggregates (Freshney et al, 2007;Labarge et al, 2007). Brehm and Spiliotis (2008) suggested that the cell arrangement around a cavity in E. multilocularis could represent a primitive lacuna in the process of cyst development.…”