Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis

Pleil, Joachim D.; Stiegel, Matthew A.; Sobus, Jon R.; Tabucchi, S.; Ghio, Andy; Madden, Michael C.

doi:10.1016/j.jchromb.2010.04.035

Cited by 67 publications

(32 citation statements)

References 53 publications

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“…From these, 134 compounds belonging to linear, ramified and cycloalkanes, alkenes, aldehydes, ketones and a group of miscellaneous compounds, were selected for further studies. The remaining compounds were considered as possible contaminants, as for example, aromatic compounds from environmental cumulative exposure [57], whereas terpenoids and esters can have its origin in ingested foods [58]. Otherwise, the linear, ramified and cycloalkanes, alkenes, aldehydes and ketones have been reported to be associated to several biochemical processes that may occur in humans [59].…”

Section: Structured Chromatogram and Sensitivitymentioning

confidence: 99%

Allergic asthma exhaled breath metabolome: A challenge for comprehensive two-dimensional gas chromatography

Caldeira

Perestrelo

Barros

et al. 2012

Journal of Chromatography A

117

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Exhaled breath Volatile metabolites Headspace-solid phase microextraction Comprehensive two-dimensional gas chromatography-time of flight mass spectrometry a b s t r a c t Allergic asthma represents an important public health issue, most common in the paediatric population, characterized by airway inflammation that may lead to changes in volatiles secreted via the lungs. Thus, exhaled breath has potential to be a matrix with relevant metabolomic information to characterize this disease. Progress in biochemistry, health sciences and related areas depends on instrumental advances, and a high throughput and sensitive equipment such as comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC × GC-ToFMS) was considered. GC × GC-ToFMS application in the analysis of the exhaled breath of 32 children with allergic asthma, from which 10 had also allergic rhinitis, and 27 control children allowed the identification of several hundreds of compounds belonging to different chemical families. Multivariate analysis, using Partial Least Squares-Discriminant Analysis in tandem with Monte Carlo Cross Validation was performed to assess the predictive power and to help the interpretation of recovered compounds possibly linked to oxidative stress, inflammation processes or other cellular processes that may characterize asthma. The results suggest that the model is robust, considering the high classification rate, sensitivity, and specificity. A pattern of six compounds belonging to the alkanes characterized the asthmatic population: nonane, 2,2,4,6,6-pentamethylheptane, decane, 3,6-dimethyldecane, dodecane, and tetradecane. To explore future clinical applications, and considering the future role of molecular-based methodologies, a compound set was established to rapid access of information from exhaled breath, reducing the time of data processing, and thus, becoming more expedite method for the clinical purposes.

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Section: Structured Chromatogram and Sensitivitymentioning

confidence: 99%

Allergic asthma exhaled breath metabolome: A challenge for comprehensive two-dimensional gas chromatography

Caldeira

Perestrelo

Barros

et al. 2012

Journal of Chromatography A

117

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“…Furthermore, many PAHs have been identified as, or suspected to be, carcinogenic, mutagenic and teratogenic. Thus, evaluating the presence of these xenobiotic classes in the environment and human body is essential for assessing and understanding their health risks [4]. …”

Section: Introductionmentioning

confidence: 99%

“…Due to their nonpolar properties, however, these molecules are generally missed by conventional MS-based metabolomics approaches. Gas chromatography-mass spectrometry (GC-MS) has been one of the most widely used methods for their analyses, but it is slow and requires additional chemical derivatization approaches which can cause degradation [4,12,16]. In most analyses, the hydroxylated metabolites are analyzed separately by liquid chromatography-mass spectrometry (LC-MS) [17,18], making multiple measurements necessary for each sample.…”

Section: Introductionmentioning

confidence: 99%

Utilizing ion mobility spectrometry and mass spectrometry for the analysis of polycyclic aromatic hydrocarbons, polychlorinated biphenyls, polybrominated diphenyl ethers and their metabolites

Zheng

Dupuis

Aly

et al. 2018

Analytica Chimica Acta

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Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent environmental pollutants originating from incomplete combustion of organic materials and synthetic sources. PAHs, PCBs, and PBDEs have all been shown to have a significant effect on human health with correlations to cancer and other diseases. Therefore, measuring the presence of these xenobiotics in the environment and human body is imperative for assessing their health risks. To date, their analyses require both gas chromatography and liquid chromatography separations in conjunction with mass spectrometry measurements for detection of both the parent molecules and their hydroxylated metabolites, making their studies extremely time consuming. In this work, we characterized PAHs, PCBs, PBDEs and their hydroxylated metabolites using ion mobility spectrometry coupled with mass spectrometry (IMS-MS) and in combination with different ionization methods including electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). The collision cross section and m/z trend lines derived from the IMS-MS analyses displayed distinct trends for each molecule type. Additionally, the rapid isomeric and molecular separations possible with IMS-MS showed great promise for quickly distinguishing the parent and metabolized PAH, PCB, and PDBE molecules in complex environmental and biological samples.

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“…As part of a series of U.S. EPA studies documenting the circulating concentrations of PAH in unremarkably exposed human subjects, either 16 or 21 distinct compounds (depending on the particular study) were measured in 373 independent blood samples (Liu and Pleil, 2001;Pleil et al, 2010). The compounds represent the suite of congeners from two-to six-ring PAH, and for this review the carcinogenic index compound benzo[a]pyrene (BaP) was selected as an example to assess cancer risk (Bostrom et al, 2002;Collins et al, 1998).…”

Section: Benzo[a]pyrene (Bap) In Bloodmentioning

confidence: 99%

Estimating Common Parameters of Lognormally Distributed Environmental and Biomonitoring Data: Harmonizing Disparate Statistics from Publications

Pleil

Sobus

Stiegel

et al. 2014

Journal of Toxicology and Environmental Health, Part B

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The progression of science is driven by the accumulation of knowledge and builds upon published work of others. Another important feature is to place current results into the context of previous observations. The published literature, however, often does not provide sufficient direct information for the reader to interpret the results beyond the scope of that particular article. Authors tend to provide only summary statistics in various forms, such as means and standard deviations, median and range, quartiles, 95% confidence intervals, and so on, rather than providing measurement data. Second, essentially all environmental and biomonitoring measurements have an underlying lognormal distribution, so certain published statistical characterizations may be inappropriate for comparisons. The aim of this study was to review and develop direct conversions of different descriptions of data into a standard format comprised of the geometric mean (GM) and the geometric standard deviation (GSD) and then demonstrate how, under the assumption of lognormal distribution, these parameters are used to answer questions of confidence intervals, exceedance levels, and statistical differences among distributions. A wide variety of real-world measurement data sets was reviewed, and it was demonstrated that these data sets are indeed of lognormal character, thus making them amenable to these methods. Potential errors incurred from making retrospective estimates from disparate summary statistics are described. In addition to providing tools to interpret "other people's data," this review should also be seen as a cautionary tale for publishing one's own data to make it as useful as possible for other researchers.

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Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis

Cited by 67 publications

References 53 publications

Allergic asthma exhaled breath metabolome: A challenge for comprehensive two-dimensional gas chromatography

Allergic asthma exhaled breath metabolome: A challenge for comprehensive two-dimensional gas chromatography

Utilizing ion mobility spectrometry and mass spectrometry for the analysis of polycyclic aromatic hydrocarbons, polychlorinated biphenyls, polybrominated diphenyl ethers and their metabolites

Estimating Common Parameters of Lognormally Distributed Environmental and Biomonitoring Data: Harmonizing Disparate Statistics from Publications

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