Cumulus co-culture system does not improve the in-vitro maturation (IVM) of oocytes in mice

Rahiminia, Tahereh; Faramarzi, Azita; Khoradmehr, Arezoo; Khalili, Mohammad Ali

doi:10.1016/j.mefs.2015.10.003

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Consistently, the present data showed how the elective use of hCG, an LH-like chorionic gonadotropin, is the unique hormonal stimulus enabling the resumption of meiosis, whereas eCG, a more FSH-like chorionic gonadotropin, did not exert any action either alone at high dose or in combination with hCG despite the contradictory literature evidence (ruminants: [ 87 , 88 , 89 , 90 , 91 ]; porcine: [ 92 , 93 ]; human: [ 94 , 95 , 96 , 97 , 98 ]; mouse: [ 44 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 ].…”

Section: Discussionmentioning

confidence: 99%

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Peserico

Berardino

Capacchietti

et al. 2023

IJMS

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In vitro maturation (IVM) is not a routine assisted reproductive technology (ART) for oocytes collected from early antral (EA) follicles, a large source of potentially available gametes. Despite substantial improvements in IVM in the past decade, the outcomes remain low for EA-derived oocytes due to their reduced developmental competences. To optimize IVM for ovine EA-derived oocytes, a three-dimensional (3D) scaffold-mediated follicle-enclosed oocytes (FEO) system was compared with a validated cumulus-oocyte complex (COC) protocol. Gonadotropin stimulation (eCG and/or hCG) and/or somatic cell coculture (ovarian vs. extraovarian-cell source) were supplied to both systems. The maturation rate and parthenogenetic activation were significantly improved by combining hCG stimulation with ovarian surface epithelium (OSE) cells coculture exclusively on the FEO system. Based on the data, the paracrine factors released specifically from OSE enhanced the hCG-triggering of oocyte maturation mechanisms by acting through the mural compartment (positive effect on FEO and not on COC) by stimulating the EGFR signaling. Overall, the FEO system performed on a developed reproductive scaffold proved feasible and reliable in promoting a synergic cytoplasmatic and nuclear maturation, offering a novel cultural strategy to widen the availability of mature gametes for ART.

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Section: Discussionmentioning

confidence: 99%

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Peserico

Berardino

Capacchietti

et al. 2023

IJMS

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“…(7) Selama proses MIV, faktor lingkungan, misalnya komposisi dan formulasi medium kultur, suplementasi protein, pH, suhu, dapat menyebabkan stress oksidatif pada oosit, yang dapat mempengaruhi pematangan inti, pembelahan, dan pembentukan blastokista. (8,9) Spesies oksigen reaktif (reactive oxygen species / ROS) diproduksi secara endogen oleh oosit selama in vitro dan in vivo. Paparan stres lingkungan selama MIV tersebut secara dramatis meningkatkan kadar ROS.…”

Section: Abstrakunclassified

Suplementasi Antioksidan pada Maturasi In Vitro Menurunkan Kadar ROS: A Literature Review

Kusumaningrum

2019

JPKSF

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One of the reproductive health problems is infertility. In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment. IVM conditions inducing a series of events related to reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic development. Therefore, the supplementation of IVM media with antioxidants could improve the survival and development of the oocytes. The method of searching conducted online database such as Science Direct, Sage, Pubmed, Springer, and Oxford. The keywords that used were “antioxidant”, “reactive oxygen species”, “in vitro maturation”, “infertility” and “assisted reproductive technology”. The literatures restricted in 2008 year until 2019 in English. The results of the study show that there were 3 main themes, types, dosages, antioxidant benefits for reduced ROS levels during IVM. The drawback that might arise in this study is that there are still possibilities of not getting some related articles. The literature review is expected to be a basis for research assisted reproductive technology. Keywords: antioxidant; in vitro maturation; infertility; Reactive oxygen species (ROS)

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Cumulus co-culture system does not improve the in-vitro maturation (IVM) of oocytes in mice

Cited by 2 publications

References 22 publications

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Suplementasi Antioksidan pada Maturasi In Vitro Menurunkan Kadar ROS: A Literature Review

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