Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture

Alberdi, Lucia Vázquez; Rosso, Gonzalo; Velóz, Lucía; Romeo, Carlos; Farías, Joaquina; Tomaso, María Vittoria Di; Calero, Miguel; Kun, Alejandra

doi:10.20944/preprints202203.0001.v1

Cited by 3 publications

(1 citation statement)

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“…Also, Heregulin (10 nM) for 24 h showed a successful mitogen for human SCs (63). The effectiveness of essential disconnected SCs with curcumin (0.25 µM) in ethanol 100% was evaluated for the alleviation of human-related peripheral neuropathies (64). In a study, the effect of the male silkworm extracts on the reproductive tract of male rats was studied.…”

Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

et al. 2023

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Background: Silkworm products were first used by physicians more than 8500 years ago, in the early Neolithic period. In Persian medicine, silkworm extract has several uses for treating and preventing neurological, cardiac, and liver diseases. Mature silkworms (Bombyx mori) and their pupae contain a variety of growth factors and proteins that can be used in many repair processes, including nerve regeneration. Objectives: The study aimed to evaluate the effects of mature silkworm (Bombyx mori), and silkworm pupae extract on Schwann cell proliferation and axon growth. Methods: Silkworm (Bombyx mori) and silkworm pupae extracts were prepared. Then, the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatograph-mass spectrometer (LC-MS/MS). Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, electron microscopy, and NeuroFilament-200 (NF-200) immunostaining. Results: According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors, such as bombyrin and laminin, in extracts that are involved in the repair of the nervous system. In accordance with Bradford's results, the evaluation of extracts using LC-MS/MS revealed that the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 mg/mL in both extracts was higher than the concentrations of 0.01 and 0.05 mg/mL. When using both extracts on dorsal root ganglion (DRGs), an increase in length and number was observed in axons. Conclusions: The findings of this study demonstrated that extracts obtained from silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration, and, consequently, repairing peripheral nerve damage.

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Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

et al. 2023

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In Vivo Ultrafast Doppler Imaging Combined with Confocal Microscopy and Behavioral Approaches to Gain Insight into the Central Expression of Peripheral Neuropathy in Trembler-J Mice

Martínez Barreiro,

Vázquez Alberdi,

De León

et al. 2023

Biology

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The main human hereditary peripheral neuropathy (Charcot-Marie-Tooth, CMT), manifests in progressive sensory and motor deficits. Mutations in the compact myelin protein gene pmp22 cause more than 50% of all CMTs. CMT1E is a subtype of CMT1 myelinopathy carrying micro-mutations in pmp22. The Trembler-J mice have a spontaneous mutation in pmp22 identical to that present in CMT1E human patients. PMP22 is mainly (but not exclusively) expressed in Schwann cells. Some studies have found the presence of pmp22 together with some anomalies in the CNS of CMT patients. Recently, we identified the presence of higher hippocampal pmp22 expression and elevated levels of anxious behavior in TrJ/+ compared to those observed in wt. In the present paper, we delve deeper into the central expression of the neuropathy modeled in Trembler-J analyzing in vivo the cerebrovascular component by Ultrafast Doppler, exploring the vascular structure by scanning laser confocal microscopy, and analyzing the behavioral profile by anxiety and motor difficulty tests. We have found that TrJ/+ hippocampi have increased blood flow and a higher vessel volume compared with the wild type. Together with this, we found an anxiety-like profile in TrJ/+ and the motor difficulties described earlier. We demonstrate that there are specific cerebrovascular hemodynamics associated with a vascular structure and anxious behavior associated with the TrJ/+ clinical phenotype, a model of the human CMT1E disease.

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Phytoconstituents of Leaves and Roots Ethanolic Extract of Talinum paniculatum and Their Biological Activities

Susilo,

Nurul Aini,

Diah Permanasari

2024

RJPT

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Javanese ginseng (Talinum paniculatum) is known to the people of Indonesia to have properties in medicine and food. People's culture generally uses leaves and roots that, until now, constituent information has yet to bereported. Therefore, variations of phytoconstituents in the leaves and roots of T. paniculatum were investigated using Gas Chromatography-Mass Spectrometry (GC-MS). The roots of T. paniculatum contain 17 active compounds dominated by N,N'-Dibutyl-N,N'-dimethylurea. The leaf has only 16 active compounds, but Python compounds from the diterpenoids class dominate it. In general, T. paniculatum contains a variety of steroid class compounds, namely Stigmasterol; Stigmast-5-en-3-ol; and 9,19-Cycloergost-24(28)-en-3-ol, 4,14-dimethyl-, acetate, (3,β., 4.α., 5.α.) - in the leaves, and stigmasterol and .γ.Sitosterol in the root. Python is known to have high efficacy as an antimicrobial, antifungal, antibacterial, antiparasitic, antimutagenic, and antioxidant. At the same time, steroid compounds are anti-cancer, antioxidant, anti-tumor, antidiabetic, and anti-inflammatory agents. The results of identifying compounds in T. paniculatum can be used as a reference in optimizing the use of T. paniculatumin the future.

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Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture

Cited by 3 publications

References 83 publications

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

In Vivo Ultrafast Doppler Imaging Combined with Confocal Microscopy and Behavioral Approaches to Gain Insight into the Central Expression of Peripheral Neuropathy in Trembler-J Mice

Phytoconstituents of Leaves and Roots Ethanolic Extract of Talinum paniculatum and Their Biological Activities

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