Curcumin and fisetin internalization into Saccharomyces cerevisiae cells via osmoporation: impact of multiple osmotic treatments on the process efficiency

Abstract

For the first time in the literature, a protocol of serial osmoporation stages to enhance the encapsulation efficiency of hydrophobic low molecular weight molecules (curcumin and fisetin) into Saccharomyces cerevisiae cells was determined. By increasing overall efficiency, this protocol empowers the encapsulation process and creates a rational way to reduce waste for future industrial osmoporation applications.

Ultrasound-assisted encapsulation of curcumin and fisetin into Saccharomyces cerevisiae cells: a multistage batch process protocol

“…In previous work by our group, successive cycles of osmoporation were able to enhance both curcumin and fisetin EEs from 14·5 to 29·5 and 33·1%; and 29·1 to 44·3% and 49·5% after 1, 2 and 3 cycles, respectively (Medeiros et al . 2018). However, yeasts have limited intracellular space to be filled with bioactive molecules.…”

“…Moreover, a comparison between our current results and those obtained by the multistage osmoporation technique of Medeiros et al . (2018) demonstrates the benefits of adding non‐loaded yeasts between each stage. These authors estimated lower increases in curcumin‐ and fisetin‐loaded content, respectively, from 0·438 to 0·733 and 0·781 mg; and from 0·742 to 0·884 and 0·900 mg after three successive osmoporation cycles using the same cell suspension during the multistage process.…”

“…Nevertheless, the loss of bioactive materials was higher than 65% for curcumin and 50% for fisetin due to the restricted yeast intracellular volume as well as the impact of osmoporation on the cell functionality (Medeiros et al . 2018). Here, the UABE protocol enabled the capture of nonencapsulated hydrophobes (above 58 and 76% for curcumin and fisetin, respectively) as well as promoted the incorporation yield of the bioparticles by adding nonloaded yeasts between each ES.…”

“…Short processing time, easier scaling‐up and higher yeast‐loading properties are desired besides the possibility to operate in a multistage batch setup to achieve enhanced internalization levels (Medeiros et al . 2018).…”

“…Otherwise, authors have proposed a broad range of bioencapsulation protocols with improved yeast permeability such as electroporation (Dimopoulos et al 2021) and osmoporation (de Medeiros et al 2019). Short processing time, easier scaling-up and higher yeastloading properties are desired besides the possibility to operate in a multistage batch setup to achieve enhanced internalization levels (Medeiros et al 2018).…”