Curcumin clarithromycin nano-form a promising agent to fight Helicobacter pylori infections

Abstract

Helicobacter pylori (H. pylori) is the main cause of gastric diseases. However, the traditional antibiotic treatment of H. pylori is limited due to increased antibiotic resistance, low efficacy, and low drug concentration in the stomach. This study developed a Nano-emulsion system with ability to carry Curcumin and Clarithromycin to protect them against stomach acidity and increase their efficacy against H. pylori. We used oil in water emulsion system to prepare a novel Curcumin Clarithromycin Nano-Emulsion (Cur-CLR-NE). The nano-emulsion was validated by dynamic light scattering (DLS) technique, zeta potential; transmission electron microscopy (mean particle size 48 nm), UV–visible scanning and Fourier transform infrared spectroscopy (FT-IR). The in vitro assay of Cur-CLR-NE against H. pylori was evaluated by minimum inhibitory concentration (12.5 to 6.26 µg/mL), minimum bactericidal concentration (MBC) and anti-biofilm that showed a higher inhibitory effect of Cur-CLR-NE in compere with, free curcumin and clarithromycin against H. pylori. The in vivo results indicated that Cur-CLR-NE showed higher H. pylori clearance effect than free clarithromycin or curcumin under the same administration frequency and the same dose regimen. Histological analysis clearly showed that curcumin is highly effective in repairing damaged tissue. In addition, a potent synergistic effect was obvious between clarithromycin and curcumin in nano-emulsion system. The inflammation, superficial damage, the symptoms of gastritis including erosion in the mouse gastric mucosa, necrosis of the gastric epithelium gastric glands and interstitial oedema of tunica muscularis were observed in the positive control infected mice and absent from treated mice with Cur-CLR-NE.

RETRACTED: Eradication of Klebsiella pneumoniae pulmonary infection by silver Oxytetracycline Nano-structure

“…Silver Oxytetracycline Nano-structure (Ag-OTC-Ns), were prepared by simple homogenization-ultrasonication method as previous described (Fernandes et al 2016;Mosallam et al 2023) with some modification. Briefly, a suspension of silver nitrate (100 µg/ml) was prepared in deionized water and added drop wise to mixture of Oxytetracycline (100 µg/ml prepared in DMSO) and 10% surfactant.…”

Eradication of Klebsiella pneumoniae pulmonary infection by silver oxytetracycline nano-structure

“…Silver Oxytetracycline Nano-structure (Ag-OTC-Ns), were prepared by simple homogenization-ultrasonication method as previous described (Fernandes et al 2016 ; Mosallam et al 2023 ) with some modification. Briefly, a suspension of silver nitrate (100 µg/ml) was prepared in deionized water and added drop wise to mixture of Oxytetracycline (100 µg/ml prepared in DMSO) and 10% surfactant.…”

Kaempferia parviflora Extracellular Vesicle Loaded with Clarithromycin for the Treatment of Helicobacter pylori Infection

“…49 This phenomenon has been demonstrated by a novel curcumin-clarithromycin nano-emulsion (Cuur-CLR-NE), which showed superior effects in abolishing gastric epithelium necrosis, reducing symptoms of gastritis, and diminishing inflammatory cell infiltration in the lamina propria. 50 Furthermore, CLA solubility increased and became more stable with a decreasing pH; the dissociation constant was 8.76. 51 Taken together, the enhanced solubility, stability, and sustained release of CLA from KPEVs in acidic environments contribute to the compatibility of KPEVs-CLA for oral administration.…”