Curcumin half analog modulates interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease

Abstract

Background:The present study was aimed at examining the effect of dehydrozingerone (DHZ), half analogue of curcumin which is the active constituent of turmeric (Curcuma longa) in the di-nitrochlorobenzene (DNCB) induced model for inflammatory bowel disease (IBD).Materials and Methods:Male Wistar rats (200–220 g) were divided into four groups (n = 6). Chemical induction of IBD was done by sensitizing with 300 µL of 20 g/L of DNCB (in acetone) onto the nape of rats for 14 days followed by intra-colonic instillation of 250 µL of DNCB (0.1% DNCB in 50% alcohol) solution on day 15. Rats in Group 1 (normal control) and Group 2 (DNCB control) were treated with vehicle. Rats in Group 3 were treated with DHZ (100 mg/kg, p.o.; 8 days) and Group 4 animals were treated with sulfasalazine (SS) (100 mg/kg, p.o.; 8 days). On 24th day, the rats were killed, colon removed and the macroscopic, biochemical, and histopathological evaluations were performed.Results:The levels of myeloperoxidase, thiobarbituric acid reactive substrate, and nitrite increased significantly (P < 0.05) in the DNCB group whereas reduced significantly in the DHZ and SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. Interleukin-6, tumor necrosis factor-alpha level was significantly high in the DNCB group.Conclusion:These findings show that DHZ can be a promising molecule for the treatment of IBD.

The Effect of Mesenchymal Stem Cells Pulsed with 17 Beta-Estradiol in an Ameliorating Rat Model of Ulcerative Colitis

“…Reduction of MDA in E2-MSCs-treated specimens might be a result of decreased oxygen and nitrogen species level in colonic tissue. Interestingly, MPO usually observed in the azurophilic granules of neutrophils can be applied in an indirect way to estimate the neutrophil content of a tissue sample similar to colonic specimens in UC condition (38). Similarly, a significant reduction in nitric oxide concentration in the E2-treated group approves the study hypothesis regarding the estrogen efficacy on MSCs proliferation in vivo.…”

An evaluation of animal models for using bioactive compounds in the treatment of inflammatory bowel disease

“…The experiment involved spraying a solution of DNCB in acetone (20 g/L and 300 μL) on the nape of rats for 14 consecutive days, followed by the instillation of 250 μL DNCB (0.1%) in a 50% ethanol solution into the rectum of rats through a 16G intravenous cannula positioned 6–8 cm away from the anus on the 15th day. Ten days after induction, the rats showed a reduction in weight, an increase in the level of inflammatory cytokines, and visible signs of colonic ulceration and congestion (Kondamudi et al., 2015). This model exhibits a high rate of success, good reproducibility, and a prolonged course of the disease.…”

Animal models of inflammatory bowel disease: category and evaluation indexes

“…SD rats are induced colitis by free drinking or enema treatment using chemical substances such as acetic acid, Trinitro-benzene-sulfonic acid (TNBS)-ethanol mixture, dextran sulfate sodium (DSS), and oxazolone [ 13 , 18 , 25–27 ], and similar induction methods are used in Wistar rats [ 28–31 ]. The dinitrobenzene sulfonic acid model and dinitrochlorobenzene model require skin sensitization prior to enema treatment [ 32 , 33 ].…”