Curcumin induced the cell death of immortalized human keratinocytes (HaCaT) through caspase-independent and caspase-dependent pathways

Song, Wei; Ren, Yuan-Jing; Liu, Lulu; Zhao, Yaying; Li, Qifu; Yang, Haibo

doi:10.1039/d1fo01560e

Cited by 11 publications

(9 citation statements)

References 36 publications

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“…At present, the United States Food and Drug Administration (FAD) has regarded it as a new anticancer drug in the 21 st century [16] . In addition, clinical trials have been conducted to gradually study the pharmacokinetics of Cur and the bioeffective dose for cancer treatment [17][18][19][20] . The research on its application in ophthalmology mainly focuses on its antiangiogenesis effect, which may play an important role in the treatment of pterygium and diabetic retinopathy [5,21] .…”

Section: Discussionmentioning

confidence: 99%

Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells

Zheng¹,

Zhong²,

Zhou³

et al. 2022

Int J Ophthalmol

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AIM: To investigate the effects of curcumin (Cur) nanoparticles loaded with chitosan derivatives grafted by deoxycholic acid (Chit-DC) on human retinal pigment epithelial (hRPE) cell proliferation and vascular endothelial growth factor (VEGF) mRNA expression. METHODS: Cur nanoparticles were synthesized with Chit-DC as the carrier and Cur as the supported drug. Cell counting kit-8 (CCK-8) method was used to detect the effects of different concentrations of Cur/Chit-DC, Chit-DC, and Cur on the proliferation of hRPE cells for different times. The changes of Cur/Chit-DC and Cur on hRPE cell cycle were determined by flow cytometry. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of VEGF in hRPE cells treated with Cur, Chit-DC and Cur/Chit-DC at 10 μg/mL for 24h. RESULTS: The chitosan derivative nanoparticles, prepared by mixing Cur and Chit-DC, were yellow. The drug released from the nanoparticles reached equilibrium after 96h, and the cumulative drug release amount was 31.6%. Different concentrations of Chit-DC nanoparticle treated hRPE cells had no significant difference in terms of optical density (OD) values compared with the control group at 24h and 48h. Moreover, there was no change in the cell morphology under a light microscope. After 24h treatment with Cur/Chit-DC and Cur, the percentage of G0-G1 phase cells increased and the percentage of S phase cells decreased in all concentration groups. Cur/Chit-DC and Cur in all concentration groups inhibited the proliferation of hRPE cells in a time and dose dependent manner, and reduced the expression level of VEGF mRNA. CONCLUSION: The Cur/Chit-DC nanoparticles can release Cur continuously and have sustained release function. Both Cur/Chit-DC nanoparticles and Cur could inhibit hRPE cells cultured in vitro, and could reduce the expression level of VEGF mRNA in hRPE cells.

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Section: Discussionmentioning

confidence: 99%

Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells

Zheng¹,

Zhong²,

Zhou³

et al. 2022

Int J Ophthalmol

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“…The immunoblot analysis demonstrating the absence of caspase cleavage during cell death also confirmed these findings. A natural phenolic compound, curcumin, has been recently reported to induce apoptosis of immortalized human keratinocytes (HaCaT) cells [ 79 ]. A 24-μM dose of circumin arrested the cells at the G2/M phase, with an apoptosis rate of 33.95%.…”

Section: Molecular Agents Inducing Caspase-independent Programmed Cel...mentioning

confidence: 99%

“…Meanwhile, after curcumin treatment, mitochondrial membrane depolarization led to cytochrome c release from the mitochondria to the cytoplasm, and the ratio of Bax to Bcl-2 in HaCaT cells was also increased, which subsequently initiated the activation of caspase-3. Taken together, these results suggested that curcumin-induced apoptosis in HaCaT cells occurs through both caspase-independent and caspase-dependent pathways [ 79 ]. Apart from the above listed natural compounds, few more natural extracts were also reported to follow both caspase-dependent and caspase-free cell death pathways.…”

Section: Molecular Agents Inducing Caspase-independent Programmed Cel...mentioning

confidence: 99%

A Mini Review on Molecules Inducing Caspase-Independent Cell Death: A New Route to Cancer Therapy

Bhadra

2022

Molecules

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Most anticancer treatments trigger tumor cell death through apoptosis, where initiation of proteolytic action of caspase protein is a basic need. But under certain circumstances, apoptosis is prevented by the apoptosis inhibitor proteins, survivin and Hsp70. Several drugs focusing on classical programmed death of the cell have been reported to have low anti-tumorogenic potency due to mutations in proteins involved in the caspase-dependent programmed cell death with intrinsic and extrinsic pathways. This review concentrates on the role of anti-cancer drug molecules targeting alternative pathways of cancer cell death for treatment, by providing a molecular basis for the new strategies of novel anti-cancer treatment. Under these conditions, active agents targeting alternative cell death pathways can be considered as potent chemotherapeutic drugs. Many natural compounds and other small molecules, such as inorganic and synthetic compounds, including several repurposing drugs, are reported to cause caspase-independent cell death in the system. However, few molecules indicated both caspase-dependent as well caspase-free cell death in specific cancer lines. Cancer cells have alternative methods of caspase-independent programmed cell death which are equally promising for being targeted by small molecules. These small molecules may be useful leads for rational therapeutic drug design, and can be of potential interest for future cancer-preventive strategies.

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“…The ratio of Bax to Bcl-2 was also increased, initiating the caspase 3 activation. These data suggest that the apoptosis induced by curcumin occurs through the caspase-dependent and caspase-independent pathways [ 84 ].…”

Section: Signaling Molecules and Biological Pathways Targeted By Plan...mentioning

confidence: 99%

Intracellular Pathways and Mechanisms of Colored Secondary Metabolites in Cancer Therapy

Sevastre

Manea

Popescu

et al. 2022

IJMS

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Despite the great advancements made in cancer treatment, there are still many unsatisfied aspects, such as the wide palette of side effects and the drug resistance. There is an obvious increasing scientific attention towards nature and what it can offer the human race. Natural products can be used to treat many diseases, of which some plant products are currently used to treat cancer. Plants produce secondary metabolites for their signaling mechanisms and natural defense. A variety of plant-derived products have shown promising anticancer properties in vitro and in vivo. Rather than recreating the natural production environment, ongoing studies are currently setting various strategies to significantly manipulate the quantity of anticancer molecules in plants. This review focuses on the recently studied secondary metabolite agents that have shown promising anticancer activity, outlining their potential mechanisms of action and pathways.

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Curcumin induced the cell death of immortalized human keratinocytes (HaCaT) through caspase-independent and caspase-dependent pathways

Abstract: Curcumin is a diketone compound found in Turmeric. It is not only used as food additives and spices, but also has anti proliferation and anti-cancer properties. However, the effect of...

Cited by 11 publications

References 36 publications

Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells

Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells

A Mini Review on Molecules Inducing Caspase-Independent Cell Death: A New Route to Cancer Therapy

Intracellular Pathways and Mechanisms of Colored Secondary Metabolites in Cancer Therapy

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