Curcumin inhibits adipogenesis induced by benzyl butyl phthalate in 3T3-L1 cells

Kim

Invest. Ophthalmol. Vis. Sci.

et al. 2019

PURPOSE. We examined the therapeutic effect of nontoxic concentrations of curcumin, a plant polyphenol extracted from Curcuma longae, in primary cultures of orbital fibroblasts from Graves' orbitopathy (GO). METHODS. The effect of curcumin on interleukin (IL)-1b induced-proinflammatory cytokine production was determined using quantitative real-time PCR, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis. Adipogenic differentiation was identified using Oil-Red O staining, and levels of peroxisome proliferator activator c (PPARc) and CCAATenhancer-binding proteins (C/EBP) a/b were determined by Western blot analyses. Antioxidant activity was measured using an oxidant-sensitive fluorescent probe to detect intracellular reactive oxygen species (ROS) generated in response to hydrogen peroxide (H 2 O 2) and cigarette smoke extract (CSE). RESULTS. Treatment with curcumin resulted in a dose-and time-dependent decrease in IL-1binduced synthesis of inflammatory cytokines, including IL-6, IL-8, MCP-1, and ICAM-1 at both mRNA and protein levels. Decrease in lipid droplets and expression of PPARc and c/EBPa/b were noted in fibroblasts treated with curcumin during adipose differentiation. CSE-or H 2 O 2induced ROS synthesis was significantly lower in curcumin-treated fibroblasts in comparison with the control. Curcumin significantly suppressed IL-1b-induced phosphorylated extracellular signal-regulated kinase, Akt, c-Jun NH(2)-terminal kinase, and nuclear factor jlight-chain-enhancer of activated B cells, p65 proteins and stimulated b-catenin translocation into nucleus during adipogenesis. CONCLUSIONS. Curcumin inhibits proinflammatory cytokine production, ROS synthesis, and adipogenesis in orbital fibroblasts of GO patients in vitro possibly related to multiple proinflammatory signaling molecules and b-catenin pathway. The results of the study support potential use of the curcumin in the treatment of GO.

Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Therapeutic Effect of Curcumin, a Plant Polyphenol Extracted From Curcuma longae, in Fibroblasts From Patients With Graves' Orbitopathy

Kim

Invest. Ophthalmol. Vis. Sci.

et al. 2019

“…Inhibiting adipogenesis of 3T3‐L1 preadipocytes may be a useful strategy to prevent obesity. Recently, various natural products have been studied for their ability to inhibit adipogenesis of 3T3‐L1 preadipocytes as an approach to explore applications of natural products for preventing obesity (Kang, Kang, Ko, et al, ; Kim, Kim, et al, ; Sakuma et al, ). The purpose of this study was to first evaluate the inhibitory effect of BD on adipogenesis and adipogenic‐specific factors in 3T3‐L1 preadipocytes and then investigate the mechanism underlying its effect.…”

Section: Discussionmentioning

confidence: 99%

Bromophenol (5‐bromo‐3,4‐dihydroxybenzaldehyde) isolated from red alga Polysiphonia morrowii inhibits adipogenesis by regulating expression of adipogenic transcription factors and AMP‐activated protein kinase activation in 3T3‐L1 adipocytes

Ding

Kim

et al. 2018

Phytotherapy Research

The aim of the present study was to investigate the effect of 5‐bromo‐3,4‐dihydroxybenzaldehyde (BD) isolated from Polysiphonia morrowii on adipogenesis and differentiation of 3T3‐L1 preadipocytes into mature adipocytes and its possible mechanism of action. Levels of lipid accumulation and triglyceride were significantly lower in BD treated cells than those in untreated cells. In addition, BD treatment reduced protein expression levels of peroxisome proliferator‐activated receptor‐γ, CCAAT/enhancer‐binding proteins α, and sterol regulatory element‐binding protein 1 compared with control (no treatment). It also reduced expression levels of adiponectin, leptin, fatty acid synthase, and fatty acid binding protein 4. AMP‐activated protein kinase activation was found to be one specific mechanism involved in the effect of BD. These results demonstrate that BD possesses inhibitory effect on adipogenesis through activating AMP‐activated protein kinase signal pathway.

“…3T3-L1 preadipocytes are extensively used as an in vitro model for studying adipogenesis. Our laboratory [12] and other groups [13] [14] [15] have reported that CUR suppresses the adipogenic differentiation of 3T3-L1 preadipocytes by downregulating the CCAAT-enhancer binding protein α (C/EBPα)-peroxisome proliferator-activated receptor γ (PPARγ) pathway, which is a major adipogenesis pathway [16] [17]. Thus, the present study was designed to determine whether CZP induces adipocyte differentiation of 3T3-L1 cells via the C/EBPα-PPARγ pathway.…”

Section: Introductionmentioning

confidence: 97%

Curcumin Inhibits Adipogenesis Elicited by Clozapine in 3T3-L1 Cells

Sakuma¹,

Sumida²,

Sakabe³

et al. 2018

FNS

Self Cite

Although clozapine (CZP), which is used for schizophrenia treatment, causes weight gain, the mechanism remains unclear. We recently reported that the naturally occurring compound curcumin (CUR) suppresses adipogenesis in 3T3-L1 cells. The aims of the present study were to determine the mechanism by which CZP induces adipocyte differentiation of 3T3-L1 cells, and whether CUR reduces CZP-induced adipogenesis. We found that cells grown in the presence of CZP had significantly higher triacylglycerol levels, numbers of lipid-filled adipocytes, and mRNA expression levels of CCAAT-enhancer binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) than those grown without CZP. Treatment with CZP plus CUR resulted in major reductions in these four parameters. These results suggest that CZP enhances adipogenesis in 3T3-L1 cells via the C/EBPα-PPARγ pathway and that by interrupting CZP's effects, CUR might be a potent agent for preventing CZP-induced weight gain.