Curcumin inhibits appoptosin-induced apoptosis via upregulating heme oxygenase-1 expression in SH-SY5Y cells

Abstract

Aim: Appoptosin (SLC25A38) is a pro-apoptotic protein, which is upregulated in Alzheimer's disease (AD) brains and plays an important role in promoting the pathological progress of AD. The aim of this study was to investigate the effects of curcumin from the rhizome of Curcuma longa on appoptosin-induced apoptosis in SH-SY5Y cells. Methods: SH-SY5Y cells were pretreated with curcumin, then transfected with appoptosin or vector. The apoptotic cells were detected with Annexin V staining analysis by flow cytometry. The expression of cleaved caspase-3, appoptosin, heme oxygenase-1 (HO-1) was examined using Western blotting. Intracellular level of ROS was measured with DCFH-DA staining by flow cytometry analysis. Mitochondrial membrane potential (ΔΨm) was detected with JC-1 staining under a fluorescence microscope and quantified by fluorescence ratio detection. Results: Overexpression of appoptosin in SH-SY5Y cells markedly increased cell apoptosis accompanied by reduced HO-1 expression, increased intracellular heme level, ROS overproduction and ΔΨm impairment. Treatment of SH-SY5Y cells with curcumin (2.5-20 μmol/L) for 24 h did not significantly affect their viability. However, pretreatment with curcumin (2.5-20 μmol/L) dose-dependently attenuated all above-mentioned pathological changes in appoptosin-transfected SH-SY5Y cells. Conclusion: Curcumin inhibits appoptosin-induced apoptosis in SH-SY5Y cells by upregulating the expression of HO-1, reducing the production of intracellular heme and ROS, and preventing the ΔΨm loss.

Curcumin Inhibits Heat-Induced Apoptosis by Suppressing NADPH Oxidase 2 and Activating the Akt/mTOR Signaling Pathway in Bronchial Epithelial Cells

“…Curcumin is a plant-derived compound shown to protect cells from apoptosis through different pathways [11]. We proceeded to test whether curcumin exerted a protective effect on heat-induced apoptosis in 16HBE140 cells.…”

“…Recent studies have indicated that long-term curcumin administration significantly inhibits oxidative stress and apoptosis in human neuroblastoma cells [11]. Curcumin has been reported to regulate the PKCd/NADPH oxidase/ROS and Akt/mTOR signaling pathways in cancer cells [12, 13].…”

Hemeoxygenase-1 Suppresses IL-1β-Induced Apoptosis Through the NF-κB Pathway in Human Degenerative Nucleus Pulposus Cells

“…For example, overexpression of HO-1 significantly decreased the number of apoptotic cells induced by high glucose in H9c2 cells [24, 25]. Moreover, upregulating hemeoxygenase-1 expression by curcumin inhibits anthracycline-induced apoptosis in SHSY5Y cells [26]. Thus, we hypothesized that HO-1 could protect degenerative human NP cells against apoptosis.…”

Curcumin alleviates glucocorticoid‐induced osteoporosis by protecting osteoblasts from apoptosis in vivo and in vitro

“…A number of studies have shown that curcumin can inhibit cell proliferation and induce apoptosis in a variety of cancer cells through multiple signalling pathways such as Wnt, the nuclear factor (NF)‐ κ B, Notch, and mitogen‐activated protein kinase (MAPK) signalling pathways . In contrast, several reports indicate that curcumin can protect cells from apoptosis in various disease models including hepatic injury, testicular injury, and Alzheimer's disease . Besides, it has been reported that curcumin can produce antidepressant effects by activating ERK signalling pathway in hippocampus .…”

“…[35][36][37] In contrast, several reports indicate that curcumin can protect cells from apoptosis in various disease models including hepatic injury, testicular injury, and Alzheimer's disease. [38][39][40] Besides, it has been reported that curcumin can produce antidepressant effects by activating ERK signalling pathway in hippocampus. 41 ERK signalling activation is essential for cell survival, during which ERK1/2 is phosphorylated and translocated to the nucleus to activate multiple downstream substrates.…”