Curcuminoids encapsulated liposome nanoparticles as a blue light emitting diode induced photodynamic therapeutic system for cancer treatment

Vetha, Berwin Singh Swami; Oh, Phil-Sun; Kim, Suhn Hee

doi:10.1016/j.jphotobiol.2020.111840

Cited by 18 publications

(10 citation statements)

References 25 publications

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“…Interestingly, SCC-25 cells revealed apoptosis as a leading cause of cell death, while MUG-Mel2 cells showed both types of cell death as a possible mechanism. The above finding remains in concordance with the results of other authors and shows that, in hydrophobic photosensitizers, an increase of photodynamic efficacy could be achieved by trapping them in liposomes [ 41 , 45 ].…”

Section: Discussionsupporting

confidence: 92%

“…In the case of a free substance, it interacts with the cellular outer membrane, while liposomes are quickly internalized and enter the cell through the endosomal route, which increases its bioavailability and, thus, results in more potent cytotoxic effects [ 43 , 44 ]. Acquired results are in accordance with the observations and conclusions of Vetha et al and Ambreen et al on different cancer cell lines [ 41 , 45 ]. Although the effect was evident for both malignant cell lines, normal HaCaT keratinocytes were slightly resistant to the therapy.…”

Section: Discussionsupporting

confidence: 92%

“…Although curcumin can be applied in a pure form and then sensitized with light at the proper wavelength, its liposomal formulation was proposed as the more effective strategy in killing the malignant cells [ 41 , 42 ]. Free curcumin is characterized by low water solubility and poor bioavailability.…”

Section: Discussionmentioning

confidence: 99%

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The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells

et al. 2021

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The research focused on the investigation of curcumin encapsulated in hydrogenated soy phosphatidylcholine liposomes and its increased photoactive properties in photodynamic therapy (PDT). The goal of this study was two-fold: to emphasize the role of a natural photoactive plant-based derivative in the liposomal formulation as an easily bioavailable, alternative photosensitizer (PS) for the use in PDT of skin malignancies. Furthermore, the goal includes to prove the decreased cytotoxicity of phototoxic agents loaded in liposomes toward normal skin cells. Research was conducted on melanoma (MugMel2), squamous cell carcinoma (SCC-25), and normal human keratinocytes (HaCaT) cell lines. The assessment of viability with MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) evaluated cell death after exposure to blue light irradiation after 4 h of pre-incubation with free and encapsulated curcumin. Additionally, the wound healing assay, flow cytometry, and immunocytochemistry to detect apoptosis were performed. The malignant cells revealed increased phototoxicity after the therapy in comparison to normal cells. Moreover, liposome curcumin-based photodynamic therapy showed an increased ratio of apoptotic and necrotic cells. The study also demonstrated that nanocurcumin significantly decreased malignant cell motility following PDT treatment. Acquired results suggest that liposomal formulation of a poor soluble natural compound may improve photosensitizing properties of curcumin-mediated PDT treatment in skin cancers and reduce toxicity in normal keratinocytes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

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The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells

et al. 2021

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“…To alleviate this hurdle, curcuminwas successfully encapsulated into liposome nanocarriers (LIP-CUR). This formulation exhibited better stability and bioactivities (Vetha et al, 2020). Encapsulation of curcumin in a nanoliposome formulation to improve its stability and bioactivity was documented (Chen et al, 2015).…”

Section: Liposomes For Bioactive Phytochemical Deliverymentioning

confidence: 97%

Advancements in liposome technology: Preparation techniques and applications in food, functional foods, and bioactive delivery: A review

Ajeeshkumar

Aneesh

Raju

et al. 2021

Comp Rev Food Sci Food Safe

182

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Liposomes play a significant role in encapsulation of various bioactive compounds (BACs), including functional food ingredients to improve the stability of core. This technology can be used for promoting an effective application in functional food and nutraceuticals. Incorporation of traditional and emerging methods for the developments of liposome for loading BACs resulted in viable and stable liposome formulations for industrial applications. Thus, the advance technologies such as supercritical fluidic methods, microfluidization, ultrasonication with traditional methods are revisited. Liposomes loaded with plant and animal BACs have been introduced for functional food and nutraceutical applications. In general, application of liposome systems improves stability, delivery, and bioavailability of BACs in functional food systems and nutraceuticals. This review covers the current techniques and methodologies developed and practiced in liposomal preparation and application in functional foods.

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“…Research group led by Vetha et al reported apoptosis of A549 cancer cells in vitro, by generating singlet oxygen, increasing intracellular ROS levels and activating Caspase-3, by using small CUR molecules, encapsulated in liposome nanoparticles (LIP-CUR) coupled with PDT via emitting diodes blue light (BLED) [161].…”

Section: Skin Cancermentioning

confidence: 99%

Latest Innovations and Nanotechnologies with Curcumin as a Nature-Inspired Photosensitizer Applied in the Photodynamic Therapy of Cancer

Ailioaie

Litscher

2021

Pharmaceutics

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In the context of the high incidence of cancer worldwide, state-of-the-art photodynamic therapy (PDT) has entered as a usual protocol of attempting to eradicate cancer as a minimally invasive procedure, along with pharmacological resources and radiation therapy. The photosensitizer (PS) excited at certain wavelengths of the applied light source, in the presence of oxygen releases several free radicals and various oxidation products with high cytotoxic potential, which will lead to cell death in irradiated cancerous tissues. Current research focuses on the potential of natural products as a superior generation of photosensitizers, which through the latest nanotechnologies target tumors better, are less toxic to neighboring tissues, but at the same time, have improved light absorption for the more aggressive and widespread forms of cancer. Curcumin incorporated into nanotechnologies has a higher intracellular absorption, a higher targeting rate, increased toxicity to tumor cells, accelerates the activity of caspases and DNA cleavage, decreases the mitochondrial activity of cancer cells, decreases their viability and proliferation, decreases angiogenesis, and finally induces apoptosis. It reduces the size of the primary tumor, reverses multidrug resistance in chemotherapy and decreases resistance to radiation therapy in neoplasms. Current research has shown that the use of PDT and nanoformulations of curcumin has a modulating effect on ROS generation, so light or laser irradiation will lead to excessive ROS growth, while nanocurcumin will reduce the activation of ROS-producing enzymes or will determine the quick removal of ROS, seemingly opposite but synergistic phenomena by inducing neoplasm apoptosis, but at the same time, accelerating the repair of nearby tissue. The latest curcumin nanoformulations have a huge potential to optimize PDT, to overcome major side effects, resistance to chemotherapy, relapses and metastases. All the studies reviewed and presented revealed great potential for the applicability of nanoformulations of curcumin and PDT in cancer therapy.

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Curcuminoids encapsulated liposome nanoparticles as a blue light emitting diode induced photodynamic therapeutic system for cancer treatment

Cited by 18 publications

References 25 publications

The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells

The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells

Advancements in liposome technology: Preparation techniques and applications in food, functional foods, and bioactive delivery: A review

Latest Innovations and Nanotechnologies with Curcumin as a Nature-Inspired Photosensitizer Applied in the Photodynamic Therapy of Cancer

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