Curcuminoids Extract, Hydrolyzed Collagen and Green Tea Extract Synergically Inhibit Inflammatory and Catabolic Mediator’s Synthesis by Normal Bovine and Osteoarthritic Human Chondrocytes in Monolayer

Abstract

The main objective of this study was to assess the in vitro effects of curcuminoids extract, hydrolyzed collagen and green tea extract in normal bovine chondrocytes and osteoarthritic human chondrocytes cultured in monolayer. This study also investigated the synergic or additive effects of these compounds. Enzymatically isolated primary bovine or human chondrocytes were cultured in monolayer until confluence and then incubated for 24 hours or 48 hours in the absence or in the presence of interleukin-1β and with or without curcuminoids extract, hydrolyzed collagen or green tea extract, added alone or in combination, at different concentrations. Cell viability was neither affected by these compounds, nor by interleukin 1β. In the absence of interleukin-1β, compounds did not significantly affect bovine chondrocytes metabolism. In human chondrocytes and in the absence of interleukin 1β, curcuminoids extract alone or in combination with hydrolyzed collagen and green tea extract significantly inhibited matrix metalloproteinase-3 production. In interleukin-1β-stimulated bovine chondrocytes, interleukin-6, inducible nitric oxide synthase, cyclooxygenase2, matrix metalloproteinase 3, a disintegrin and metalloproteinase with thrombospondin type I motifs 4 and a disintegrin and metalloproteinase with thrombospondin type I motifs 5 expressions were decreased by curcuminoids extract alone or in combination with hydrolyzed collagen and green tea extract. The combination of the three compounds was significantly more efficient to inhibit interleukin-1β stimulated matrix metalloproteinase-3 expression than curcuminoids extract alone. In interleukin-1β-stimulated human chondrocytes, nitric oxide, interleukin-6 and matrix metalloproteinase 3 productions were significantly reduced by curcuminoids extract alone or in combination with hydrolyzed collagen and green tea extract. These findings indicate that a mixture of curcuminoids extract, hydrolyzed collagen and green tea extract has beneficial effects on chondrocytes culture in inflammatory conditions and provide a preclinical basis for the in vivo testing of this mixture.

The spice for joint inflammation: anti-inflammatory role of curcumin in treating osteoarthritis

“… 58 , 59 , 67 , 68 Comblain et al demonstrated that curcuminoids decreased the gene expressions of MMP-3, ADAMTS4, ADAMTS5, COX-2, PGE-2, and IL-6. 56 They also found that the combination of curcuminoids, hydrolyzed collagen, and green tea extract were more effective in suppressing these genes than curcuminoids alone. 56 Currently, curcumin reduced the level of MMP-3 and aggrecans released into the media by the bovine chondrocytes.…”

“… 56 They also found that the combination of curcuminoids, hydrolyzed collagen, and green tea extract were more effective in suppressing these genes than curcuminoids alone. 56 Currently, curcumin reduced the level of MMP-3 and aggrecans released into the media by the bovine chondrocytes. 56 This was in contrast with the findings of Mathy-Hartert et al, whereby production of aggrecans by chondrocytes was not affected by curcumin.…”

“…The mechanistic studies on the antiosteoarthritic properties of curcumin were performed on primary chondrocytes isolated from human, 56 – 61 bovine, 56 canine, 62 equine, 63 or leporine 64 cartilage, or immortal cell line. 65 Cultures of chondrocyte monolayer and explant have been attempted.…”

“… 56 Currently, curcumin reduced the level of MMP-3 and aggrecans released into the media by the bovine chondrocytes. 56 This was in contrast with the findings of Mathy-Hartert et al, whereby production of aggrecans by chondrocytes was not affected by curcumin. 68 Furthermore, they showed that curcumin was able to inhibit proteoglycan degradation in human chondrocytes in basal state but not in IL-1β stimulated model.…”

Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial

“…Polyphenol's benefits on health may be potentialized by other nutrients. For instance, the combination of curcuminoid extract, hydrolyzed collagen, and green tea extract was proven to be significantly more efficient in inhibiting interleukin-1 β-stimulated matrix metalloproteinase-3 expression than curcuminoid extract alone in normal bovine chondrocytes and osteoarthritic human chondrocytes cultures [31,32]. The same authors described a trended reduction of pain in an OA model when dogs were fed with the corresponding mixture for 3 months [33].…”