Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice

Abstract

Background Curcumol exhibits anti-inflammatory effect, but its effect on chronic asthma lacked research. Therefore, this study explored the role of curcumol in asthma. Methods A chronic asthmatic mice model was established by ovalbumin induction. After treatment with curcumol, airway resistance in mice was detected by forced oscillation technique. The histopathological features of airway tissues, pulmonary inflammation, and inflammation cell recruitment in the bronchoalveolar lavage fluid (BALF) of mice were detected by hematoxylin-eosin staining. Collagen deposition in the airways of mice was examined by Masson staining. The secretion of ovalbumin-IgE, IL-4, IL-5, IL-13 in mouse serum and VEGFA secretion in BALF were analyzed by ELISA. Finally, the expressions of β-catenin, Wnt5a, VEGFA, TGF-β1, Fibronectin, and MMP-9 in mice lung tissues were determined by Western blot or immunohistochemical. Results Curcumol attenuated airway hyperresponsiveness, airway remodeling, and pulmonary inflammation in chronic asthmatic mice. Curcumol relieved collagen deposition in airway tissues, inflammation cell recruitment in BALF, and reduced the up-regulation of serum ovalbumin-IgE, IL-4, IL-5, and IL-13 and BALF VEGFA in chronic asthmatic mice. In addition, curcumol attenuated the up-regulated expressions of β-catenin, Wnt5a, VEGFA, TGF-β1, Fibronectin, and MMP-9 in the lung tissues of chronic asthmatic mice, but curcumol treatment did not show such effects on healthy mice. Conclusion Our findings revealed that curcumol could ameliorate lung inflammation and airway remodeling by inhibiting the abnormal activation of the Wnt/β-catenin pathway in chronic asthmatic mice, indicating that curcumol could be used as a novel anti-asthma drug for basic and clinical research.

Curcumol inhibits PDGF-BB-induced proliferation and migration of airway smooth muscle cells by suppressing ERK/CREB pathway

“…17,21-24 Importantly, Jia et al have demonstrated that curcumol helps in treating airway remodeling and lung inflammation in chronic asthmatic mice. 25 However, the in vitro experimental effect of curcumol on asthma has not been studied. Therefore, this study detected the effect of curcumol on PDGF-BB-stimulated ASMCs.…”

“…24 Moreover, curcumol has been proved to ameliorate airway remodeling and lung inflammation in a mouse model of chronic asthma (8). 25 However, the molecular mechanisms of curcumol in asthma are rarely reported.…”

To Predict Anti-Inflammatory and Immunomodulatory Targets of Guizhi Decoction in Treating Asthma Based on Network Pharmacology, Molecular Docking, and Experimental Validation

“…Asthma, featured by airway inflammation, is inseparable from inflammation and immune disorders. Jia et al found that curcumol improved pneumonia and airway remodeling in mice with chronic asthma by inhibiting the Wnt/ β -catenin pathway through experimental studies [ 37 ]. Lyu et al found that Pinellia ternata reduced the allergic response to asthma in mice by inhibiting the activation of TH2 cells and the expression of the inflammatory factor IL-4 [ 38 ].…”

Network Pharmacology and Comparative Transcriptome Reveals Biotargets and Mechanisms of Curcumol Treating Lung Adenocarcinoma Patients With COVID-19

“…An in vitro study by Li et al showed that curcumol suppresses proliferation of the LUAD cells, A549 and H460, by arresting the cell cycle, altering the expression of apoptosis signaling pathways and inducing tumor cell apoptosis ( 14 ). In chronic asthmatic mice, curcumol was found to reduce pulmonary inflammation and airway remodeling by decreasing cytokine levels ( 15 ). In addition, curcumol was reported to inhibit LUAD growth and metastasis and overcome tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) resistance in lung cancer ( 16 ).…”