CuReSim-LoRM: A Tool to Simulate Metabarcoding Long Reads
Yasmina Mesloub,
Delphine Beury,
Félix Vandermeeren
et al.
Abstract:Metabarcoding DNA sequencing has revolutionized the study of microbial communities. Third-generation sequencing producing long reads had opened up new perspectives. Obtaining the full-length ribosomal RNA gene would permit one to reach a better taxonomic resolution at the species or the strain level. However, Oxford Nanopore Technologies (ONT) sequencing produces reads with high error rates, which introduces biases in analysis. Understanding the biases introduced during the analysis allows one to better interp… Show more
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