Current achievements and future prospects of genetic engineering in Indian mustard (Brassica juncea L. Czern &amp; Coss.)

Thakur, Ajay Kumar; Parmar, Nehanjali; Singh, K. H.; Nanjundan, J.

doi:10.1007/s00425-020-03461-8

Cited by 35 publications

(19 citation statements)

References 78 publications

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“…(1986) reported good amenability to tissue culture of the Indian lines, with greater potential for adventitious shoot regeneration than the European lines. Since the regeneration capacity is the precondition for A. tumefaciens ‐mediated transformation, most of the transformation experiments in B. juncea reported so far involved Indian lines (Ahmed et al., 2017; Bhuiyan et al., 2011; Dutta et al., 2008; Mondal et al., 2007; Rani et al., 2017; Sharma et al., 2004; Singh et al., 2009; Thakur et al., 2020). This paper is one of the first reporting successful A. tumefaciens ‐mediated transformation of a European line of B. juncea .…”

Section: Discussionmentioning

confidence: 99%

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Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9

et al. 2021

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SUMMARY Food allergies are a major health issue worldwide. Modern breeding techniques such as genome editing via CRISPR/Cas9 have the potential to mitigate this by targeting allergens in plants. This study addressed the major allergen Bra j I, a seed storage protein of the 2S albumin class, in the allotetraploid brown mustard (Brassica juncea). Cotyledon explants of an Indian gene bank accession (CR2664) and the German variety Terratop were transformed using Agrobacterium tumefaciens harboring binary vectors with multiple single guide RNAs to induce either large deletions or frameshift mutations in both Bra j I homoeologs. A total of 49 T0 lines were obtained with up to 3.8% transformation efficiency. Four lines had large deletions of 566 up to 790 bp in the Bra j IB allele. Among 18 Terratop T0 lines, nine carried indels in the targeted regions. From 16 analyzed CR2664 T0 lines, 14 held indels and three had all four Bra j I alleles mutated. The majority of the CRISPR/Cas9‐induced mutations were heritable to T1 progenies. In some edited lines, seed formation and viability were reduced and seeds showed a precocious development of the embryo leading to a rupture of the testa already in the siliques. Immunoblotting using newly developed Bra j I‐specific antibodies revealed the amount of Bra j I protein to be reduced or absent in seed extracts of selected lines. Removing an allergenic determinant from mustard is an important first step towards the development of safer food crops.

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Section: Discussionmentioning

confidence: 99%

“…Factors that could improve the transformation efficiencies in future experiments could involve a delayed start of selection for 3–5 days (as shown by Bhuiyan et al., 2011) or a pre‐culture of 2 days as suggested by Thakur et al. (2020).…”

Section: Discussionmentioning

confidence: 99%

Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9

et al. 2021

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“…Seeds (Figure 32) of the plant contains Quercetin, predominate, kaempferol, luteolin, apigenin indole-3-carbinol. Seed oil is used as hair oil and useful for hair nourishment [79][80].…”

Section: Brassica Junceamentioning

confidence: 99%

Ethnopharmacological relevance’s of herbal plants used in cosmetics and toiletries preparations

Motule¹,

More²,

Manwar

et al. 2021

GSC Biol. and Pharm. Sci.

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There are thousands of herbs that are used in preparation of various herbal cosmetics and toiletries preparations. Day-by-day, use of herbal cosmetics and toiletries preparations are increasing as currently available cosmetics are showing unwanted effects upon prolong used. They are prepared by using different herbs or their extracts or juice derived from them. Herbal cosmetics and toiletries preparations are being used by Indian peoples and peoples from Indian sub-continental region since ancient time. They are used to improve the appearance of the skin. Herbal cosmetics are becoming more popular because they are comparatively cheaper, safe and easily available. WHO as well as AYUSH department of India, encourages the peoples to use these types of cosmetics for routine use. In the present paper, we have covered various pharmacognostic and relevant information of medicinal plants used in the cosmetics and toiletries preparations.

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“…Phytoremediation is an ecologically and economically sound strategy to eliminate heavy metal ions from contaminated soils [ 20 ]. Brassica juncea is frequently used for the remediation of soils contaminated with heavy metals, owing to its high ability to fix metal ions in aboveground plant parts [ 21 , 22 ]. Several metal transporters have been identified in B. juncea .…”

Section: Introductionmentioning

confidence: 99%

Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L.

Han

Zhang

et al. 2022

BMC Plant Biol

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Background Brassica juncea behaves as a moderate-level accumulator of various heavy metal ions and is frequently used for remediation. To investigate the roles of metal ion transporters in B. juncea, a cation-efflux family gene, BjCET1, was cloned and functionally characterized. Results BjCET1 contains 382 amino acid residues, including a signature motif of the cation diffusion facilitator protein family, six classic trans-membrane-spanning structures and a cation-efflux domain. A phylogenetic analysis showed that BjCET1 has a high similarity level with metal tolerance proteins from other Brassica plants, indicating that this protein family is highly conserved in Brassica. BjCET1 expression significantly increased at very early stages during both cadmium and zinc treatments. Green fluorescence detection in transgenic tobacco leaves revealed that BjCET1 is a plasma membrane-localized protein. The heterologous expression of BjCET1 in a yeast mutant increased the heavy-metal tolerance and decreased the cadmium or zinc accumulations in yeast cells, suggesting that BjCET1 is a metal ion transporter. The constitutive expression of BjCET1 rescued the heavy-metal tolerance capability of transgenic tobacco plants. Conclusions The data suggest that BjCET1 is a membrane-localized efflux transporter that plays essential roles in heavy metal ion homeostasis and hyper-accumulation.

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Current achievements and future prospects of genetic engineering in Indian mustard (Brassica juncea L. Czern & Coss.)

Cited by 35 publications

References 78 publications

Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9

Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9

Ethnopharmacological relevance’s of herbal plants used in cosmetics and toiletries preparations

Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L.

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