Current challenges in non-invasive insulin delivery systems: A comparative review

Khafagy, El-Sayed; Morishita, Mariko; Onuki, Yoshinori; Takayama, K.

doi:10.1016/j.addr.2007.08.019

Cited by 379 publications

(260 citation statements)

References 260 publications

Supporting

Mentioning

256

Contrasting

Unclassified

Order By: Relevance

“…Additionally, following oral administration, vaccines are extremely nonresistant to enzymatic deterioration in the stomach and intestinal environment. 7 Once entrapped in suitable carrier nanoparticles, antigens will be protected versus the degradative condition of the GI environment prior to their absorption in their intact form. Furthermore, using mucoadhesive polymers for the preparation of the delivery vehicle lengthens the association of the particles to the surface of mucosa while achieving the greater regional concentration of antigen in the mucus bed and minimizing dilution and destruction of antigen by the intestinal lumen contents.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, using mucoadhesive polymers for the preparation of the delivery vehicle lengthens the association of the particles to the surface of mucosa while achieving the greater regional concentration of antigen in the mucus bed and minimizing dilution and destruction of antigen by the intestinal lumen contents. 7,8 Chitosan polymer is derived from chitin by deacetylation. It is widely investigated for applications in pharmaceutical domains.…”

Section: Introductionmentioning

confidence: 99%

“…The HPMCP free carboxyl groups have the pKa of about 5.2. 7,8 The charge of free carboxylic acid groups of this polymer is converted into negative at pH values of higher than 5.2 and serves as the enforcement for the electrostatic bond with TMC. 8 The objectives of the present work was to prepare TMC/ HPMCP nanoparticles using the ionic gelation technique by cross-linking pH-sensitive HPMCP polymer with TMC and to evaluate the utility of the nanoparticles in the oral delivery of HBsAg.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Oral vaccination is the preferred route of immunization. However, the degradative condition of the gastrointestinal tract and the higher molecular size of peptides pose major challenges in developing an effective oral vaccination system. One of the most excellent methods used in the development of oral vaccine delivery system relies on the entrapment of the antigen in polymeric nanoparticles. In this work, trimethyl chitosan (TMC) nanoparticles were fabricated using ionic gelation teqnique by interaction hydroxypropyl methylcellulose phthalate (HPMCP), a pHsensitive polymer, with TMC and the utility of the particles in the oral delivery of hepatitis B surface antigen (HBsAg) was evaluated employing solutions that simulated gastric and intestinal conditions. The particle size, morphology, zeta potential, loading capacity, loading efficiency, in vitro release behavior, structure, and morphology of nanoparticles were evaluated, and the activity of the loaded antigen was assessed. Size of the optimized TMC/HPMCP nanoparticles and that of the antigen-loaded nanoparticles were 85 nm and 158 nm, respectively. Optimum loading capacity (76.75%) and loading efficiency (86.29%) were achieved at 300 mg/mL concentration of the antigen. SEM images revealed a spherical shape as well as a smooth and near-homogenous surface of nanoparticles. Results of the in vitro release studies showed that formulation with HPMCP improved the acid stability of the TMC nanoparticles as well as their capability to preserve the loaded HBsAg from gastric destruction. The antigen showed good activity both before and after loading. The results suggest that TMC/HPMCP nanoparticles could be used in the oral delivery of HBsAg vaccine.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

show abstract

“…Some diabetic patients may not comply with this approach because of anxiety, inconvenience, needle phobia, pain, risk of infection and dermatologic reactions such as itching, swelling, and atrophy or hypertrophy of adipose tissue at the injection site (Snow, 2009). Various routes of administration have been widely investigated to be an alternative of insulin delivery (Heller et al, 2007;Khafagy et al, 2007). One of the interesting routes is an oral administration because it is convenient, non-invasive and patient-friendly.…”

Section: Introductionmentioning

confidence: 99%

“…This can mimic the physiological fate of endogenous insulin which is transferred from pancreas to liver via hepatic portal system (Heller et al, 2007;Sabetsky & Ekblom, 2010). Nevertheless, insulin is degraded by the gastrointestinal enzymes and the absorption of insulin through gastrointestinal membrane is also limited by its hydrophilic macromolecular property (Heller et al, 2007;Khafagy et al, 2007). Several approaches have been proposed such as absorption enhancers, enzyme inhibitors, carrier-based delivery systems, including liposomes, niosomes, micro-and nanoparticles to overcome these barriers for oral insulin delivery (Heller et al, 2007;Khafagy et al, 2007;Sabetsky & Ekblom, 2010).…”

Section: Introductionmentioning

confidence: 99%

Hypoglycemic activity and stability enhancement of human insulin–tat mixture loaded in elastic anionic niosomes

et al. 2016

View full text Add to dashboard Cite

This study aimed to investigate the synergistic effect of trans-activator of transcription (Tat) and niosomes for the improvement of hypoglycemic activity of orally delivered human insulin. The elastic anionic niosomes composing of Tween 61/cholesterol/dicetyl phosphate/sodium cholate at 1:1:0.05:0.02 molar ratio loaded with insulin-Tat mixture (1:3 molar ratio) was prepared. Deformability of the elastic anionic niosomes decreased after loaded with the mixture of 1.35 times. For the in vitro release, the insulin (T 10 ¼ 4 h) loaded in the elastic anionic niosomes indicated the slower release rate than insulin in the mixture (T 10 ¼ 3 h) loaded in niosomes. At room temperature (30 ± 2 C), the mixture loaded in elastic anionic niosomes was more chemical stable than the free mixture of 1.3, 1.4 and 1.7 times after stored for 4, 8 and 12 weeks, respectively. Oral administration in the alloxan-induced diabetic mice of the mixture loaded in elastic anionic niosomes with the insulin doses at 25, 50 and 100 IU/kg body weight indicated significant hypoglycemic activity with the percentage fasting blood glucose reduction of 1.95, 2.10 and 2.10 folds of the subcutaneous insulin injection at 12 h, respectively. This study has demonstrated the synergistic benefits of Tat and elastic anionic niosomes for improving the hypoglycemic activity of the orally delivered human insulin as well as the stability enhancement of human insulin when stored at high temperature. The results from this study can be further developed as an effective oral insulin delivery.

show abstract

Challenges in Delivery of Biopharmaceuticals; the Need for Advanced Delivery Systems

Nielsen

Jørgensen

2009

Delivery Technologies for Biopharmaceuticals

View full text Add to dashboard Cite

Current challenges in non-invasive insulin delivery systems: A comparative review

Cited by 379 publications

References 260 publications

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Hypoglycemic activity and stability enhancement of human insulin–tat mixture loaded in elastic anionic niosomes

Challenges in Delivery of Biopharmaceuticals; the Need for Advanced Delivery Systems

Contact Info

Product

Resources

About