Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products

Khare, Saumya; Prakash, Om

doi:10.1016/j.jclepro.2017.05.040

Cited by 15 publications

(6 citation statements)

References 4 publications

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“…The investigation of biopolymers has been reserved for biochemists and molecular biologists for over half of a century. Nevertheless, during the last decade, the soft matter physics, biophysics, chemists, and material scientists have been seized to this research field [ 1 ]. The current interest in developing novel bio-based materials has motivated an increasing need for biological and medical studies for a variety of clinical applications [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

et al. 2020

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Two bio-based polymers have been compared in this study, namely: polylactide (PLA) and polyhydroxyoctanoate (PHO). Due to their properties such as biocompatibility, and biointegrity they are considered to be valuable materials for medical purposes, i.e., creating scaffolds or wound dressings. Presented biopolymers were investigated for their impact on cellular migration strategies of mouse embryonic fibroblasts (MEF) 3T3 cell line. Advanced microscopic techniques, including confocal microscopy and immunofluorescent protocols, enabled the thorough analysis of the cell shape and migration. Application of wound healing assay combined with dedicated software allowed us to perform quantitative analysis of wound closure dynamics. The outcome of the experiments demonstrated that the wound closure dynamics for PLA differs from PHO. Single fibroblasts grown on PLA moved 1.5-fold faster, than those migrating on the PHO surface. However, when a layer of cells was considered, the wound closure was by 4.1 h faster for PHO material. The accomplished work confirms the potential of PLA and PHO as excellent candidates for medical applications, due to their properties that propagate cell migration, vitality, and proliferation—essential cell processes in the healing of damaged tissues.

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Section: Introductionmentioning

confidence: 99%

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

et al. 2020

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“…β-Glucosidases are produced by a myriad of microorganisms, plants, and animals . Glucosidase derived from the fungus A.…”

Section: Resultsmentioning

confidence: 99%

“…42 ■ RESULTS AND DISCUSSION β-Glucosidases are produced by a myriad of microorganisms, plants, and animals. 43 Glucosidase derived from the fungus A. niger is commonly employed because of the relatively high kinetic activity observed with this particular isozyme. 44 As seen in Figure S1, the commercially available crude product contains at least four protein fractions in the molecular weight range studied, not surprising given that over 170 genes in A. niger encode proteins capable of biomass degradation.…”

Section: ■ Experimental Sectionmentioning

confidence: 87%

Enhanced Activity of the Cellulase Enzyme β-Glucosidase upon Addition of an Azobenzene-Based Surfactant

Seidel

Lee

2020

ACS Sustainable Chem. Eng.

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β-Glucosidases catalyze the hydrolysis of cellobiose to glucose, which is often the rate-limiting step in the conversion of cellulose into fermentable sugars during bioethanol production. Thus, the structure and function of β-glucosidase from Aspergillus niger were examined in response to a photoresponsive azobenzene-based surfactant (4ethyl-4′(trimethylamino-butoxy)azobenzene bromide, azoTAB) as a means to enhance the enzyme activity. Light and neutron scattering data indicate that pure β-glucosidase exists as dimers or higher aggregates in solution that are progressively converted to monomers with an increasing azoTAB concentration. This transition is accompanied by a 60% increase in catalytic activity. In contrast, the enzyme is simply deactivated in the presence of conventional straight-chain hydrocarbon surfactants. Shape-reconstructed images obtained from SANS data demonstrate that azoTAB causes selective unfolding in the α/β sandwich domain that comprises the crystallographic dimer interface, consistent with the observed transition to monomers. Furthermore, this domain forms one side of a long cleft that begins at the active site and facilitates the nonproductive binding of substrate or longer oligosaccharides, which at times can block the active site. Indeed, kinetic data indicate that the azoTAB-induced increase in β-glucosidase activity is a result of diminished substrate inhibition, thus providing a unique means of obtaining glucose-tolerant β-glucosidases.

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“…These polymers are structurally simple macromolecules, chemically are linear in a head to tail formation of ( R )‐3‐hydroxyalkanoic acids with the carboxyl group of one monomer forming an ester bond with the hydroxyl group of the neighboring monomer . Production of PHAs by various microorganisms including wild‐type and engineered bacteria has been extensively investigated . Due to the diverse chemical structures they are highly versatile and present a broad range of physical and chemical properties allowing them to become an attractive alternative to commonly used materials in biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Cellular architecture and migration behavior of fibroblast cells on polyhydroxyoctanoate (PHO): A natural polymer of bacterial origin

et al. 2019

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Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products

Cited by 15 publications

References 4 publications

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

Enhanced Activity of the Cellulase Enzyme β-Glucosidase upon Addition of an Azobenzene-Based Surfactant

Cellular architecture and migration behavior of fibroblast cells on polyhydroxyoctanoate (PHO): A natural polymer of bacterial origin

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