Current Diagnostic Methods of HER-2/neu Detection in Breast Cancer With Special Regard to Real-Time PCR

Merkelbach‐Bruse, Sabine; Wardelmann, Eva; Behrens, P.; Losen, Inge; Buettner, Reinhard; Friedrichs, Nicolaus

doi:10.1097/00000478-200312000-00010

Cited by 29 publications

(21 citation statements)

References 20 publications

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“…Previous studies have shown an equivalence of 80-90% among FISH and qPCR techniques on samples assessed as 3+ by IHC (9,10). In this sense, our results confirm the same percentage of accuracy, as we only had one discrepancy among the eight samples analyzed (ID 14).…”

Section: Resultssupporting

confidence: 87%

Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies

2012

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Abstract. her-2 gene amplification and its overexpression in breast cancer cells is directly associated with aggressive clinical behavior. The her-2 gene and its Her-2 protein have been utilized for disease diagnosis and as a predictive marker for treatment response to the antibody herceptin. Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common FDA-approved methodologies involving gene and protein quantification, respectively. False positive or negative her-2/Her-2 patient results may result in inappropriate treatment administration. To support accurate quantification and interpretation of results, in this study we have standardized qPCR analysis using previously identified IHC samples, obtaining very significant and clinically useful results.

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Section: Resultssupporting

confidence: 87%

Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies

2012

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“…20 In addition, a tissue microarray containing 93 cases of invasive female breast cancer with detailed followup data of 70 cases comprising 30 years was included in our study. All 144 cases were analyzed immunohistochemically using commercially available antibodies directed against AP-2alpha and AP2gamma.…”

Section: Resultsmentioning

confidence: 99%

“…The study collective has been intensively characterized previously with respect to tumor stage, tumor grade and HER2/neu status. 20 In addition, a tissue microarray containing 93 additional cases collected at the Department of Gynecology and Obstetrics, University of Heidelberg, Germany was analyzed. To account intratumoral heterogeneity two tissue samples per case have been mounted on the tissue microarray.…”

Section: Patientsmentioning

confidence: 99%

Distinct spatial expression patterns of AP-2alpha and AP-2gamma in non-neoplastic human breast and breast cancer

et al. 2005

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Although transcription factors AP-2alpha and AP-2gamma have been implicated in the control of estrogen receptor (ER) and ErbB-2, their impact for breast cancer is still controversial. To better understand the role of AP-2 proteins in mammary neoplasia, the analysis of their spatial expression pattern in normal breast and breast cancer is required. A total of 51 specimens of female breast cancer patients and a tissue microarray containing 93 additional female breast cancer cases were immunohistochemically stained for AP-2alpha, AP2gamma, ER and ErbB-2. In 70 cases of the tissue microarray, survival data comprising a period of up to 30 years were present. In non-neoplastic breast tissue, AP-2alpha was expressed in the inner glandular cell layer while AP-2gamma was expressed in the outer myoepithelial cell layer. Ductal carcinoma in situ revealed strongly AP-2alpha-positive tumor cells surrounded by a layer of AP-2gamma-positive myoepithelial cells. In invasive carcinoma, expression of AP-2alpha and AP-2gamma was variable. High expression of ER and AP2alpha showed better survival rates than low expression of these markers. AP-2gamma expression had no effect on survival. These results for the first time reveal a distinct spatial expression pattern of AP-2alpha and AP-2gamma in normal breast and in ductal carcinoma in situ with specific AP-2gamma expression in myoepithelium. High ER and AP-2alpha expression in invasive breast cancer showed favorable survival rates. Therefore, AP-2alpha expression seems to be associated with better prognosis of breast cancer. AP-2gamma expression has no influence on survival reflecting that myoepithelial cells are not involved in the neoplastic process.

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“…Good concordance, however, was reported between FISH and Qrt-PCR on the DNA as well as the RNA level (34,35).…”

Section: Correlation Between the Her2 Status Assessed Either By Qrt-pmentioning

confidence: 94%

Simultaneous Quantitative Detection of Relevant Biomarkers in Breast Cancer by Quantitative Real-Time PCR

Labuhn¹,

Vuaroqueaux²,

Fina

et al. 2006

Int J Biol Markers

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Current Diagnostic Methods of HER-2/neu Detection in Breast Cancer With Special Regard to Real-Time PCR

Cited by 29 publications

References 20 publications

Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies

Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies

Distinct spatial expression patterns of AP-2alpha and AP-2gamma in non-neoplastic human breast and breast cancer

Simultaneous Quantitative Detection of Relevant Biomarkers in Breast Cancer by Quantitative Real-Time PCR

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