Current frontiers and recommendations for the study of microplastics in seafood

Dehaut, Alexandre; Hermabessière, Ludovic; Duflos, Guillaume

doi:10.1016/j.trac.2018.11.011

Cited by 165 publications

(98 citation statements)

References 101 publications

(272 reference statements)

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“…To reduce the potential external contamination of the samples, cotton lab coats and cleaned latex gloves were worn at all times; all materials were rinsed with distillated water prior to usage; all surfaces were wiped clean and glass and metal equipment and containers were preferred over plastic materials (24). For every sample batch a distillated water and 10% KOH solution blanks were prepared by filling and incubating two glass test tubes.…”

Section: Quality Controlmentioning

confidence: 99%

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Microplastic abundance in three commercial fish from the coast of Lima, Peru

De-la-Torre¹,

Dioses-Salinas²,

Pérez-Baca³

et al. 2019

Braz. J. Nat. Sci

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Microplastics (< 5 mm) are widespread within the marine environment, posing a major threat to marine biota. The aim of the present study was to investigate microplastic contamination in three widespread and highly commercial fish from the coast of Lima, Peru. Peruvian silverside (Odontesthes regia), Peruvian morwong (Cheilodactylus variegatus), and Peruvian grunt (Anisotremus scapularis) specimens were caught off the coast of Lima. Fish stomach and intestines were extracted and submerged in 10% (w/v) KOH, followed by 24 h incubation at 60 °C. The resulting supernatant solution was vacuum filtrated and filters were then observed under an optical microscope and stereomicroscope. Strict quality control and external contamination prevention measures were taken. Microplastic abundance, type and color were recorded. Quality control measures resulted in reduced external contamination. C. variegatus was the most contaminated fish (5.13 ± 0.81 MP/individual), similar to A. scapularis (5.00 ± 0.46 MP/individual), but significantly different to O. regia (0.43 ± 0.11 MP/individual). Fibers were the overall most abundant microplastic type, while blue the dominant color. Results indicated highly contaminated fish, compared to those from other parts of the SE Pacific. Microplastic ingestion by C. variegatus and A. scapularis may be subject to trophic transfer from their natural prey. Highly populated cities and poor solid waste management may contribute to worsen microplastic ingestion by native marine species. The need for further research and a marine microplastic monitoring program was discussed.

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Section: Quality Controlmentioning

confidence: 99%

“…For every sample batch a distillated water and 10% KOH solution blanks were prepared by filling and incubating two glass test tubes. A wet filter paper was placed close to the working table for the time the laboratory analysis lasted and later observed under the stereomicroscope to determine airborne external contamination (24).…”

Section: Quality Controlmentioning

confidence: 99%

Microplastic abundance in three commercial fish from the coast of Lima, Peru

De-la-Torre¹,

Dioses-Salinas²,

Pérez-Baca³

et al. 2019

Braz. J. Nat. Sci

View full text Add to dashboard Cite

show abstract

“…For modelling and mass balance studies and their monitoring, Py-GC/MS is the method of choice while mass calculations based on FTIR particle counts need to be further investigated prior to future use to avoid a high risk of failure in the presence of many large particles of currently up to more than one order of magnitude. In an optimal workflow, a combination of both techniques should be used for identification and quantification [3,75]. Here, ideally, the same samples are analyzed in a direct succession.…”

Section: Additional Aspectsmentioning

confidence: 99%

Comparison of pyrolysis gas chromatography/mass spectrometry and hyperspectral FTIR imaging spectroscopy for the analysis of microplastics

et al. 2020

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Analysis of microplastics (MP) in environmental samples is an emerging field, which is performed with various methods and instruments based either on spectroscopy or thermoanalytical methods. In general, both approaches result in two different types of data sets that are either mass or particle number related. Depending on detection limits of the respective method and instrumentation the derived polymer composition trends may vary. In this study, we compare the results of hyperspectral Fourier-transform infrared (FTIR) imaging analysis and pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) analysis performed on a set of environmental samples that differ in complexity and degree of microplastic contamination. The measurements were conducted consecutively, and on exactly the same sample. First, the samples were investigated with FTIR using aluminum oxide filters; subsequently, these were crushed, transferred to glass fiber filters, in pyrolysis cups, and measured via Py-GC/MS. After a general data harmonization step, the trends in MP contamination were thoroughly investigated with regard to the respective sample set and the derived polymer compositions. While the overall trends in MP contamination were very similar, differences were observed in the polymer compositions. Furthermore, polymer masses were empirically calculated from FTIR data and compared with the Py-GC/MS results. Here, a most plausible shape-related overestimation of the calculated polymer masses was observed in samples with larger particles and increased particle numbers. Taking into account the different measurement principles of both methods, all results were examined and discussed, and future needs for harmonization of intermethodological results were identified and highlighted.

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“…Interestingly, in the case of Py-GC/MS, retention times for alkanes can be easily obtained after pyrolysis of PE, allowing to get rid of the expensive n-paraffin mixes. Additionally, the introduction of a single PE sample in each analytical batch could both participate to use a positive identification control (PIC) as proposed by Dehaut et al (2019) and to use retention times of alkanes for LRI calculations.…”

Section: Py-gc/ms Qa/qcmentioning

confidence: 99%

Microplastics Detection Using Pyrolysis-GC/MS-Based Methods

Dehaut

Hermabessière

Duflos

2020

Handbook of Microplastics in the Environment

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By now the polymeric nature of microparticles has to be demonstrated before their classification as microplastics. Pyrolysis coupled to gas chromatography and mass spectrometry (Py-GC/MS) has been used for years to characterize polymers. Thanks to three distinct steps, destructuring, separation, and identification, this chapter explains how this technique allows the analyst to ascertain the polymeric

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Current frontiers and recommendations for the study of microplastics in seafood

Cited by 165 publications

References 101 publications

Microplastic abundance in three commercial fish from the coast of Lima, Peru

Microplastic abundance in three commercial fish from the coast of Lima, Peru

Comparison of pyrolysis gas chromatography/mass spectrometry and hyperspectral FTIR imaging spectroscopy for the analysis of microplastics

Microplastics Detection Using Pyrolysis-GC/MS-Based Methods

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