Current insights into the microbial degradation for pyrethroids: strain safety, biochemical pathway, and genetic engineering

Zhao, Tianye; Hu, Kaidi; Li, Jianlong; Zhu, Yongxu; Liu, Aiping; Yao, Kai; Liu, Shuliang

doi:10.1016/j.chemosphere.2021.130542

Cited by 33 publications

(16 citation statements)

References 151 publications

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“…Co-culture of JQ-L and A-3 could completely degrade cypermethrin. Compared with JQ-L alone, co-culture of JQ-L and A-3 significantly reduced the total degradation time of cypermethrin from 60 to 24 h. Furthermore, in previous reports (Chen et al, 2012b, Liu et al, 2014Zhan et al, 2020;Zhao et al, 2021), it took 72 h or more for coculture of cypermethrin-degrading strain and 3-PBA-degrading strain to completely degrade 0.24 mM of cypermethrin, while in this study, it took only 24 h for co-culture of JQ-L and A-3 to completely degrade the same amount of cypermethrin. The possible reasons for the high degradation efficiency of co-culture of JQ-L and A3 were that the two strains were isolated from the same consortium, and their growth conditions were similar, so they could grow well at the some culture system; secondly, JQ-L had enzymes that converted cypermethrin to 3-PBA, while A-3 has enzymes that completely degraded 3-PBA, thus, co-culture of JQ-L and A3 possessed a complete catabolic enzymes system; furthermore, when A3 was co-cultured with JQ-L, it degraded and removed the metabolite 3-PBA thus eliminated the toxicity and inhibition effect of 3-PBA on JQ-L, thereby significantly improving the efficiency of cypermethrin degradation by JQ-L.…”

Section: Discussionmentioning

confidence: 68%

Highly efficient degradation of cypermethrin by a co-culture of Rhodococcus sp. JQ-L and Comamonas sp. A-3

Chen

Wang³

et al. 2022

Front. Microbiol.

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Cypermethrin is an important synthetic pyrethroid pesticide that widely used to control pests in agriculture. However, extensive use has caused its residue and the metabolite 3-phenoxybenzoic acid (3-PBA) to seriously pollute the environments and agricultural products. In this study, a highly efficient cypermethrin-degrading bacterial consortium was acclimated from long-term pyrethroid-contaminated soil. Two strains, designated JQ-L and A-3, were screened from the consortium, and identified as Rhodococcus sp. and Comamonas sp., respectively. Strain JQ-L transformed 100 mg/L of cypermethrin to 3-PBA within 60 h of incubation; however, 3-PBA could not be further degraded by the strain. Strain A-3 utilized 3-PBA as sole carbon for growth, and completely degraded 100 mg/L of 3-PBA within 15 h of incubation. Co-culture of JQ-L and A-3 completely degraded 100 mg/L of cypermethrin within 24 h of incubation. Furthermore, a complete catabolic pathway of cypermethrin and the metabolite 3-PBA by the co-culture was proposed. This study provided a promising strategy for efficient elimination of cypermethrin residue-contaminated environments and agricultural products.

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Section: Discussionmentioning

confidence: 68%

Highly efficient degradation of cypermethrin by a co-culture of Rhodococcus sp. JQ-L and Comamonas sp. A-3

Chen

Wang³

et al. 2022

Front. Microbiol.

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“…However, Gordonella has not been reported to degrade pyrethroid pesticides, so there is still room for exploration and development of the biodegradation ability of pyrethroid pesticides by Gordonella. In addition, Bacillus, Pseudomonas, Raoultella, Brevibacterium, Aspergillus, Acinetobacter, Candida, and Trichoderma have been characterized on the basis of biochemical and molecular tools for their pyrethroid degradation potential in soil and water environments [28][29][30][31][32][33][34][35]. Our study provides the first evidence that Gordonella bacterial strains participate in highly effective degradation of tetramethrin and other pyrethroids.…”

Section: Growth Linked Degradation Of Tetramethrin With Strain A16mentioning

confidence: 89%

Novel Mechanism and Kinetics of Tetramethrin Degradation Using an Indigenous Gordonia cholesterolivorans A16

Guo

Huang

Pang

et al. 2021

IJMS

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Tetramethrin is a pyrethroid insecticide that is commonly used worldwide. The toxicity of this insecticide into the living system is an important concern. In this study, a novel tetramethrin-degrading bacterial strain named A16 was isolated from the activated sludge and identified as Gordonia cholesterolivorans. Strain A16 exhibited superior tetramethrin degradation activity, and utilized tetramethrin as the sole carbon source for growth in a mineral salt medium (MSM). High-performance liquid chromatography (HPLC) analysis revealed that the A16 strain was able to completely degrade 25 mg·L−1 of tetramethrin after 9 days of incubation. Strain A16 effectively degraded tetramethrin at temperature 20–40 °C, pH 5–9, and initial tetramethrin 25–800 mg·L−1. The maximum specific degradation rate (qmax), half-saturation constant (Ks), and inhibition constant (Ki) were determined to be 0.4561 day−1, 7.3 mg·L−1, and 75.2 mg·L−1, respectively. The Box–Behnken design was used to optimize degradation conditions, and maximum degradation was observed at pH 8.5 and a temperature of 38 °C. Five intermediate metabolites were identified after analyzing the degradation products through gas chromatography–mass spectrometry (GC-MS), which suggested that tetramethrin could be degraded first by cleavage of its carboxylester bond, followed by degradation of the five-carbon ring and its subsequent metabolism. This is the first report of a metabolic pathway of tetramethrin in a microorganism. Furthermore, bioaugmentation of tetramethrin-contaminated soils (50 mg·kg−1) with strain A16 (1.0 × 107 cells g−1 of soil) significantly accelerated the degradation rate of tetramethrin, and 74.1% and 82.9% of tetramethrin was removed from sterile and non-sterile soils within 11 days, respectively. The strain A16 was also capable of efficiently degrading a broad spectrum of synthetic pyrethroids including D-cyphenothrin, chlorempenthrin, prallethrin, and allethrin, with a degradation efficiency of 68.3%, 60.7%, 91.6%, and 94.7%, respectively, after being cultured under the same conditions for 11 days. The results of the present study confirmed the bioremediation potential of strain A16 from a contaminated environment.

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“…The strong antimicrobial activities of 3-phenoxy-benzaldehyde prevent some bacterial species from further degradation of pyrethroid and thereby decrease the efficiency of biodegradation (Tyler et al 2000 ). Remarkably, all isolates were able to further degrade 3-phenoxy-benzaldehyde to 1-phenoxy-2-propanol, and isopropyl 4-hydroxybenzoate by aromatic ring cleavage as well as phenol by diphenyl ether cleavage and subsequent catabolism (Cycon and Piotrowska-Seget 2016 ; Hu et al 2019 ; Zhao et al 2021 ). The production of phenol and phenyl ethanoic acid, benzoic acid from 3-phenoxy-benzaldehyde has been observed in the two previous cyhalothrin biodegradation studies by B. thuringiensis (Chen et al 2015 ) and by bacterial consortium of Bacillus species, Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Detection of high amounts of aliphatic acids such as oleic acid, erucic acid and other enoic acids confirmed complete degradation of λ-cyhalothrin by the bacterial isolates. Bacteria employ the pyrethroid degradation to generate energy by Krebs cycle (Zhao et al 2021 ). These results were in line with the high efficiency of all tested isolates in the λ-cyhalothrin biodegradation compared to other studies in literature.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, SPs can be degraded through both abiotic pathways, including photo-oxidation and chemical oxidation and biotic pathways mainly by soil microflora (Guo et al 2009 ). Microorganisms-induced degradation makes it easier for SPs remediation because it is flexible, cost-effective, and better for the environment (Zhao et al 2021 ). Microorganisms characterized by their ability to degrade one or two kinds of pesticides have been isolated and identified (Cycon and Piotrowska-Seget 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous biodegradation of λ-cyhalothrin pesticide and Vicia faba growth promotion under greenhouse conditions

2022

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Abstractλ-cyhalothrin is a widely used synthetic pyrethroid insecticide and its persistence in plant, soil and water exerts a detrimental effect on humans as well as the environment. There are many studies regarding isolated bacteria capable of degrading λ-cyhalothrin in vitro. However, limited work has been done examining the microbial degradation of λ-cyhalothrin together with plant growth promotion under greenhouse conditions. In this study, 43 bacterial strains were isolated from heavily polluted soil with λ-cyhalothrin by the enrichment technique. The plant growth promotion characteristics of all isolates were evaluated. The results revealed that five isolates were potential in λ-cyhalothrin biodegradation at high concentration (1200 mg/L) within only 24 h together with their high plant growth promotion abilities. The morphological, biochemical and 16S rDNA sequence analyses identified the isolates as Bacillus subtilis strains. The GC/MS analysis revealed that the selected isolates reached high levels of degradation after only two days, the degradation percentage ranged from 95.72 to 99.52% after 48 h of incubation. Furthermore, the degradation pathway for complete detoxification and metabolism of λ-cyhalothrin was established. Moreover, greenhouse experiment was conducted, the results indicate that the application of seed coat significantly enhanced Vicia faba seedling growth and caused an increase from 38.4 to 40.2% percentage of fresh and dry weight, respectively compared to untreated control. All isolates were effective to remove the pesticide residues in Vicia faba seedlings and recorded the highest degradation percentage of 83.79 under greenhouse conditions. Therefore, it can be concluded that the Bacillus subtilis strains isolated in this study have a dual potential role in complete mineralization of λ-cyhalothrin residues in vivo as well as effective biofertilization for future use in sustainable agriculture.

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Current insights into the microbial degradation for pyrethroids: strain safety, biochemical pathway, and genetic engineering

Cited by 33 publications

References 151 publications

Highly efficient degradation of cypermethrin by a co-culture of Rhodococcus sp. JQ-L and Comamonas sp. A-3

Highly efficient degradation of cypermethrin by a co-culture of Rhodococcus sp. JQ-L and Comamonas sp. A-3

Novel Mechanism and Kinetics of Tetramethrin Degradation Using an Indigenous Gordonia cholesterolivorans A16

Simultaneous biodegradation of λ-cyhalothrin pesticide and Vicia faba growth promotion under greenhouse conditions

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