Current Issues in Cereal Crop Biodiversity

Moreta, Danilo; Mathur, P.N.; Zonneveld, Maarten van; Amaya, Karen; Arango, Jacobo; Selvaraj, Michael Gomez; Dedičová, Beata

doi:10.1007/10_2013_263

Cited by 5 publications

(3 citation statements)

References 131 publications

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“…Isoprenoid biosynthesis provides an additional route to energy-dense C 5 alcohols, namely isopentenol (3-methyl-3- and 3-methyl-2-buten-1-ol, also known as isoprenol and prenol, respectively) and isopentanol (3-methyl-1-butanol) 7 . These alcohols have octane numbers and combustion properties that make them potential gasoline replacements 8 .…”

mentioning

confidence: 99%

Metabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli

George

Thompson

Kang

et al. 2015

Sci Rep

133

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Branched five carbon (C5) alcohols are attractive targets for microbial production due to their desirable fuel properties and importance as platform chemicals. In this study, we engineered a heterologous isoprenoid pathway in E. coli for the high-yield production of 3-methyl-3-buten-1-ol, 3-methyl-2-buten-1-ol, and 3-methyl-1-butanol, three C5 alcohols that serve as potential biofuels. We first constructed a pathway for 3-methyl-3-buten-1-ol, where metabolite profiling identified NudB, a promiscuous phosphatase, as a likely pathway bottleneck. We achieved a 60% increase in the yield of 3-methyl-3-buten-1-ol by engineering the Shine-Dalgarno sequence of nudB, which increased protein levels by 9-fold and reduced isopentenyl diphosphate (IPP) accumulation by 4-fold. To further optimize the pathway, we adjusted mevalonate kinase (MK) expression and investigated MK enzymes from alternative microbes such as Methanosarcina mazei. Next, we expressed a fusion protein of IPP isomerase and the phosphatase (Idi1~NudB) along with a reductase (NemA) to diversify production to 3-methyl-2-buten-1-ol and 3-methyl-1-butanol. Finally, we used an oleyl alcohol overlay to improve alcohol recovery, achieving final titers of 2.23 g/L of 3-methyl-3-buten-1-ol (~70% of pathway-dependent theoretical yield), 150 mg/L of 3-methyl-2-buten-1-ol, and 300 mg/L of 3-methyl-1-butanol.

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mentioning

confidence: 99%

Metabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli

George

Thompson

Kang

et al. 2015

Sci Rep

133

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“…More than 22,211 pearl millet and 35,382 finger millet have been collected and consolidated in different gene banks with the largest collection at ICRISAT. Of these, a few have been utilized as breeding/research material [29]. Despite these numbers, conservation of millets in genebanks in SSA and globally is generally compared with other crop species.…”

Section: Millets Genetic Resources and Improvementmentioning

confidence: 99%

Millets: Traditional “Poor Man’s” Crop or Future Smart Nutri-Cereals?

Bombom¹,

Kaweesi²,

Walugembe³

et al. 2024

Millets - Rediscover Ancient Grains

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Millets represent a diverse group of cereal crops of significance to sub-Saharan Africa and globally. However, they remain a set of crops with limited attention and priority paid to them with paucity of information on their genetic diversity and sustainable use. Existing knowledge on millets with respect to cultivation, health, and nutritional benefits, and contribution to sustainable environmental management, and use is mainly attributed to traditional indigenous knowledge held by rural folks in different regions of the continent. The emergence of other cereal staples, however, led to millets losing their place as an important crop limiting their use to a “famine” crop with production occurring on smallholdings among the marginalized poor. This threatens interest, patronage, conservation and use to sustainably and fully exploit the potential of millets for the benefit of society. Intertwined with tradition and culture, millets in sub-Saharan Africa and elsewhere nonetheless hold great promise to contribute to food security, revitalize and diversify diets, improve farmer livelihoods, resilience, and adaptation to climate change. This chapter discusses the importance of millets, challenges to production, contribution to nutrition and health, traditional knowledge and products, novel and non-traditional products, contribution to resilience and climate change, and diversity of available genetic resources.

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“…Використання генетичних ресурсів рослин для виробництва продовольства і ведення сільського господарства -центральне питання в контексті сталого розвитку, змін клімату і досягнення продовольчої безпеки. Крім підвищення врожайності сільськогосподарських культур генетично модифіковані рослини здатні зменшити тиск інтенсифікації рослинництва та скорочення сівозмін на біорізноманіття, забезпечити використання ґрунтів із низьким рівнем родючості [23].…”

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