Current Methodologies in Hybridoma Formation

Bartal, Arie H.; Hirshaut, Yashar

doi:10.1007/978-1-4612-4826-2_1

Methods of Hybridoma Formation 1987

DOI: 10.1007/978-1-4612-4826-2_1

|View full text |Cite

Current Methodologies in Hybridoma Formation

Arie H. Bartal¹,

Yashar Hirshaut²

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1989

2022

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 86 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Competitive ELISA for detection of native ras gene‐related products in sera of cancer patients

Epelbaum

Bartal

Rivkin

et al. 1989

Clinical Laboratory Analysis

View full text Add to dashboard Cite

A solid phase, enzyme-linked immunosorbent assay (ELISA) competition kit was developed to detect circulating native ras gene-related products in sera of 151 healthy volunteers and cancer patients. This assay uses monoclonal antibody (mAb) BST-6A generated against a yeast-derived, native ras-related polypeptide Yp20. Only 2% (1 of 58) of normal control sera showed strong competition, a s compared to 15% (5 of 34) of patients with early stage or no evidence of disease, and 44% (26 of 59) of patients with advanced disease. These differences were statistically significant (x2, P < 0.05-0.001). Eleven sera samples of cancer patients found to be strong competitors in the ELISA competition kit were tested for the presence of anti-ras antibodies by ELISA. None showed higher ELISA values as compared with pooled normal human serum and control sera. It is thus suggested that our procedure detected circulating ras-related oncoproteins in sera of cancer patients mainly with advanced disease.

show abstract

Competitive ELISA for detection of native ras gene‐related products in sera of cancer patients

Epelbaum

Bartal

Rivkin

et al. 1989

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

Optimization study of plasmonic cell fusion

Belansky

Yelin

2022

Sci Rep

View full text Add to dashboard Cite

Artificial cell fusion often serves as a valuable tool for studying different applications in biology and medicine, including natural development, immune response, cancer metastasis and production of therapeutic molecules. Plasmonic cell fusion, a technique that uses specific cell labeling by gold nanoparticles and resonant femtosecond pulse irradiation for fusing neighboring cells, has been demonstrated useful for such applications, allowing high cell specificity and an overall low toxicity. Despite these advantages, the numerous experimental factors contributing to plasmonic fusion have often led to subpar fusion efficiencies, requiring repeated experiments and extensive calibration protocols for achieving optimal results. In this work we present a study that aims to improve the overall performance of plasmonic cell fusion in terms of fusion efficiency and cell viability. By varying the pulse fluence, nanoparticle concentration, incubation times, and culture handling protocols, we demonstrate up to 100% fusion of malignant epithelial cells across the entire irradiated area of the culture. We also show that some of the smaller cells may stay viable for up to several days. The results would allow plasmonic fusion to play a key role in numerous studies and applications that require specific, high-efficiency cell–cell fusion.

show abstract

Radioantibodies for the Diagnosis and Treatment of Cancer; Radioimmunoimaging (RAI) and Radioimmunotherapy (RAT)

Sfakianakis

Gartyx

Serafini

1990

Cancer Investigation

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Current Methodologies in Hybridoma Formation

Cited by 3 publications

References 86 publications

Competitive ELISA for detection of native ras gene‐related products in sera of cancer patients

Competitive ELISA for detection of native ras gene‐related products in sera of cancer patients

Optimization study of plasmonic cell fusion

Radioantibodies for the Diagnosis and Treatment of Cancer; Radioimmunoimaging (RAI) and Radioimmunotherapy (RAT)

Contact Info

Product

Resources

About