Current Methods for Determination of L-Carnitine and Acylcarnitines

Möder, Monika; Kießling, Andreas; Löster, Heinz

doi:10.1007/s00706-005-0320-0

Cited by 17 publications

(14 citation statements)

References 2 publications

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“…In order to get more insight into carnitine metabolism and synthesis, a sensitive analysis for the determination of the concentrations of free carnitine, carnitine esters and the carnitine precursors trimethyllysine and ␥-butyrobetaine is required. Currently, carnitine is mostly analyzed by LC-MS/MS methods [3][4][5][6][7][8]. The use of multi-reaction mode (MRM) transitions for detection allows the determination of underivatized carnitine and acylcarnitines with sufficient sensitivity [6].…”

Section: Introductionmentioning

confidence: 99%

Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and γ-butyrobetaine by quasi-solid phase extraction and MS/MS detection

Hirche

Fischer

Keller

et al. 2009

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and γ-butyrobetaine by quasi-solid phase extraction and MS/MS detection

Hirche

Fischer

Keller

et al. 2009

Journal of Chromatography B

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“…This is particularly relevant because ovarian cancer is the seventh most common cancer in women worldwide and causes more deaths than any other gynecologic malignancy, being diagnosed mostly at an advanced stage 21 22 . There are several analytical methods to determine CRT in several samples, suitably reviewed 23 24 25 . Few of these methods offer portability features and the ones nearing it do not combine disposable supports with stable biorecognition materials, which are essential conditions to promote worldwide POC applications in early screening of ovarian cancer.…”

mentioning

confidence: 99%

Conductive Paper with Antibody-Like Film for Electrical Readings of Biomolecules

Tavares

Ferreira

Truta

et al. 2016

Sci Rep

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This work reports a novel way of producing an inexpensive substrate support to assemble a sensing film, designed for the electrical transduction of an intended biomolecule. The support uses cellulose paper as substrate, made hydrophobic with solid wax and covered by a home-made conductive ink having graphite as core material. The hydrophobicity of the paper was confirmed by contact angle measurements and the conductive ink composition was optimized with regard to its adhesion, conductivity, and thermal stability. This support was further modified targeting its application in quantitative analysis. Carnitine (CRT) was selected as target compound, a cancer biomarker. The recognition material consisted of an antibody-like receptor film for CRT, tailored on the support and prepared by electrically-sustained polymerization of 3,4-ethylenedioxythiophene (EDOT) or dodecylbenzenesulfonic acid (DBS). Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy analysis confirmed the presence of the polymeric film on the support, and the performance of the devices was extensively evaluated with regard to linear response ranges, selectivity, applicability, and reusability. Overall, the paper-based sensors offer simplicity of fabrication, low cost and excellent reusability features. The design could also be extended to other applications in electrical-based approaches to be used in point-of-care (POC).

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“…Compared with the normal rats, the levels of several glucose metabolism intermediates (such as isocitric acid and glucose) were elevated in the samples collected from THBSS rats at 2 h, 5 h, 8 h and 12 h and reduced at 24 h, 36 h and 48 h. The levels of 3-dehydroxycarnitine related to b-oxidation of long-chain fatty acids down-regulated in the samples collected at 2 h, 5 h, 8 h and 12 h and up-regulated at 24 h, 36 h and 48 h. These changes indicate excessive energy consumption in the excessive heat and little blood stasis stage and the blockade of the energy metabolism. 19 The levels of N-acetylglutamine and N-a-acetylcitrulline, which are associated with nervous system functions, were elevated in the samples collected at 2 h, 5 h, 8 h and 12 h and reduced at 24 h, 36 h and 48 h, suggesting that the nervous system is disturbed in THBSS. 20,21 The levels of iduronic acid and homocysteine sulnic acid, which can affect coagulation function, were altered, demonstrating the dysfunction of coagulation during THBSS progression.…”

Section: Metabonomics Analysismentioning

confidence: 99%

Holistic and dynamic metabolic alterations of traditional Chinese medicine syndrome in a toxic heat and blood stasis syndrome rat model

Shen

et al. 2017

RSC Adv.

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Metabonomic analysis has been widely used in the study of traditional Chinese medicine (TCM) syndromes.In this paper, dynamic changes of the metabolic network during the evolution of a syndrome based on the toxic heat and blood stasis syndrome (THBSS) rat model have been elucidated for the first time. It was found that the metabolic trajectories could characterize three stages during THBSS evolution: the metabolic disturbance at 2-5 h, 8-12 h and 24-48 h could reflect the status of the excessive heat and little blood stasis stage, the coexistence of heat and blood stasis stage and the blood stasis stage respectively. The obtained dynamic metabolic network revealed the mechanism of THBSS progression: the inhibition of nicotinate and nicotinamide metabolism may lead to the activation of SIRT1 producing an inflammatory response, which then results in the disorder of energy metabolism and amino acid metabolism, and eventually the blood stasis appears. Nine involved metabolic pathways were found, among which the nicotinate and nicotinamide metabolism and histidine metabolism closely related to the occurrence of the inflammatory response in the early stage and blood stasis in the later stage of THBSS respectively.The former one, which is not reported in other blood stasis syndromes, might be the characteristic pathway of THBSS. This study firstly reveals the essence of THBSS progression, which is conducive to the diagnosis and pharmacotherapeutics of THBSS and related diseases, and also provides a new way to study TCM syndrome evolution.

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Current Methods for Determination of L-Carnitine and Acylcarnitines

Cited by 17 publications

References 2 publications

Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and γ-butyrobetaine by quasi-solid phase extraction and MS/MS detection

Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and γ-butyrobetaine by quasi-solid phase extraction and MS/MS detection

Conductive Paper with Antibody-Like Film for Electrical Readings of Biomolecules

Holistic and dynamic metabolic alterations of traditional Chinese medicine syndrome in a toxic heat and blood stasis syndrome rat model

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