Current methods of analysis for the determination of trichothecene mycotoxins in food

Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods

“…Overestimation of Fusarium mycotoxins by immunoassays was known from previous studies targeting DON in other countries (Meneely et al, 2011;Lattanzio et al, 2009). An essential requirement for an immunoassay is the antibody specificity.…”

“…Many studies described the successful production of monoclonal or polyclonal antibodies against the trichothecenes, but often the cross-reactivity profile is not ideal and can lead to overestimation and increased uncertainty in the measurements (Meneely et al, 2011). This is true for DON where most literature highlights that antibodies raised against deoxynivalenol show strong cross-reactivity to 3-acetyldeooxynivalenol, 15-acetyldeoxynivalenol or both (Meneely et al, 2011). The antibodies designed for DON may also cross-react against DON-3β-glucoside (DON-3G) and monoacetylated derivatives (3-ADON) (Tangni et al, 2010).…”

Simultaneous determination of type-A and type-B trichothecenes in barley samples by GC–MS

“…Regulations with respect to these toxins are still being considered. Discussion limit for the sum of T-2 and HT-2 was 100 µg kg -1 for unprocessed cereals and cereals products (Meneely, Ricci, van Egmond and Elliott, 2010).…”

“…Several analytical methods for the determination and quantification of trichothecenes in food and feed commodities have been described in the reference literature, which have subsequently been reviewed by some researchers (Krska, Baumgartner and Josephs, 2001;Meneely, Ricci, van Egmond and Elliott, 2010;Langseth and Rundberget, 1998;Krska and Molinelli, 2007;Lattanzio, Pascale and Visconti, 2009 reported for the determination of DON and NIV in corn-based food products (Castillo, Montes, Navarro, Segarra, Cuesta and Hernández, 2008), for the simultaneous analysis of five type-A and -B trichothecenes (T-2, HT-2, DAS, DON and NIV) in wheat flour (Sospedra, Blesa, Soriano and Manes, 2010), and for the determination of DON, T-2 and HT-2 in cereal-based food (Cano-Sancho et al, 2010 …”

Effect of Enzymatic Deacetylation of T-2 Toxin on the Analysis of T-2 and HT-2 Toxins in Corn and Brown Rice

“…이 가운데 GC 및 HPLC를 이용한 방법들이 주로 이용되고 있으 나 정확성(accuracy), 정밀성(precision) 등의 측면에서 개선이 필 요하기 때문에 아직 GC 및 HPLC를 기반으로 한 공인분석법이 없는 실정이다 (10,11).…”