Current molecular dynamics opinions on interactions between bone morphogenetic protein-2 and inorganic materials

Huang, Baolin

doi:10.1016/j.mtcomm.2022.105307

Cited by 6 publications

(3 citation statements)

References 115 publications

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“…Ca‐TMA is a 2D crystalline structure, also known as an extended coordination polymeric structure. [ 18 ] Unlike metal‐organic framework (MOF) structures, where XRD peaks appear at 2θ < 10°, the XRD pattern of pristine Ca‐TMA (Figure S1B) as a coordination polymer shows high crystallinity with major peaks at 2θ = 24.9°, 26.8°, and 30.8°. The extended packing of the Ca‐TMA 2D coordination polymer is shown in Figure S2 (supporting information), where Ca is coordinated with four types of O atoms: a single O binding with two oxygens of a TMA‐COO‐ functional group, a single binding with one oxygen atom of a TMA‐COO‐ functional group, a bridging oxygen with a neighboring Ca‐TMA polyhydron, and lastly with an O atom of a water molecule.…”

Section: Resultsmentioning

confidence: 99%

“…Ca-TMA is a 2D crystalline structure, also known as an extended coordination polymeric structure. [18] Unlike metal-organic framework confirming that the graphene used contains less than 10 graphene layers per particle, with sp 2 -hybridized carbon. [19] For CGTase@GNP, weight losses of 3% and 11.7% were observed for Regions I and II, respectively.…”

Section: Characterizationmentioning

confidence: 90%

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Immobilization of Cyclodextrin glycosyltransferase onto three dimensional‐ hydrophobic and two dimensional‐ hydrophilic supports: A comparative study

Ogunbadejo,

Aljahoushi,

Alzamly

et al. 2023

Biotechnology Journal

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Cyclodextrin glycosyltransferase (CGTase) degrades starch into cyclodextrin via enzymatic activity. In this study, we immobilize CGTase from Thermoanaerobacter sp. on two supports, namely graphene nanoplatelets (GNP) consisting of short stacks of graphene nanoparticles and a calcium‐based two‐dimensional metal organic framework (Ca‐TMA). The uptakes of CGTase on GNP and Ca‐TMA reached 40 and 21 mg g−1 respectively, but immobilized CGTase on Ca‐TMA showed a higher specific activity (38 U mg−1) than that on GNP (28 U mg−1). Analysis of secondary structures of CGTase, shows that immobilization reduces the proportion of β‐sheets in CGTase from 56% in the free to 49% and 51.3% for GNP and Ca‐TMA respectively, α‐helix from 38.5% to 18.1 and 37.5%, but led to increased β‐turns from 5.5 to 40% and 11.2% for GNP and Ca‐TMA, respectively. Lower levels of conformational changes were observed over the more hydrophilic Ca‐TMA compared to hydrophobic GNP, resulting in its better activity. Increased β‐turns were found to correlate with lower β‐CD production, while more β‐sheets and α‐helix favored more β‐CD. Reusability studies revealed that GNP retains up to 74% of initial CGTase activity, while Ca‐TMA dropped to 33% after eight consecutive uses. The results obtained in this work provide insight on the effect of support's surface properties on CGTase performance and can assist in developing robust CGTase‐based biocatalysts for industrial application.

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Section: Resultsmentioning

confidence: 99%

Section: Characterizationmentioning

confidence: 90%

Immobilization of Cyclodextrin glycosyltransferase onto three dimensional‐ hydrophobic and two dimensional‐ hydrophilic supports: A comparative study

Ogunbadejo,

Aljahoushi,

Alzamly

et al. 2023

Biotechnology Journal

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“…Despite this, BMP-2 has a few disadvantages, such as a short half-life time, side effects when overdosed, etc. The in vivo osteoinductive efficiency of BMP-2 highly depends on its delivery method [19]. The burst release of the BMP-2 fails to induce a sustained osteogenic response at the implantation site [20,21].…”

Section: Introductionmentioning

confidence: 99%

BMP-2 Incorporated into a Biomimetic Coating on 3D-Printed Titanium Scaffold Promotes Mandibular Bicortical Bone Formation in a Beagle Dog Model

Liu

Wei

et al. 2022

SSRN Journal

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BMP2 Binds Non‐Specifically to PEG‐Passivated Biomaterials and Induces pSMAD 1/5/9 Signalling

Pennec,

Guibert,

Gurram

et al. 2024

Macromolecular Bioscience

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Biomaterials are widely employed across diverse biomedical applications and represent an attractive strategy to explore how extracellular matrix components influence cellular response. In this study, the previously developed streptavidin platforms is aimed to use to investigate the role of glycosaminoglycans (GAGs) in bone morphogenetic protein 2 (BMP2) signaling. However, it is observed that the interpretation of findings is skewed due to the GAG‐unrelated, non‐specific binding of BMP2 on components of biomaterials. Non‐specific adsorption of proteins is a recurrent and challenging issue for biomaterial studies. Despite the initial incorporation of anti‐fouling polyethylene glycol (PEG) chains within biomaterials, the residual non‐specific BMP2 adsorption still triggered BMP2 signaling within the same range as conditions of interest. The various options are explored to prevent BMP2 non‐specific adsorption and a successful blocking condition involving a combination of bovine serum albumin and trehalose are identified. Furthermore, the effect of this blocking step improved when using gold platforms instead of glass, particularly with Chinese hamster ovary (CHO) cells. With this specific example, it is suggested that non‐specific adsorption of BMPs on biomaterials may be a general concern – often undetected by classical surface‐sensitive techniques – that needs to be addressed to better interpret cellular responses.

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Current molecular dynamics opinions on interactions between bone morphogenetic protein-2 and inorganic materials

Cited by 6 publications

References 115 publications

Immobilization of Cyclodextrin glycosyltransferase onto three dimensional‐ hydrophobic and two dimensional‐ hydrophilic supports: A comparative study

Immobilization of Cyclodextrin glycosyltransferase onto three dimensional‐ hydrophobic and two dimensional‐ hydrophilic supports: A comparative study

BMP-2 Incorporated into a Biomimetic Coating on 3D-Printed Titanium Scaffold Promotes Mandibular Bicortical Bone Formation in a Beagle Dog Model

BMP2 Binds Non‐Specifically to PEG‐Passivated Biomaterials and Induces pSMAD 1/5/9 Signalling

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