Current Options in the Management of Colorectal Cancer in Developing Countries: Central America Experience

Ochoa-Hernandez, A.; Giron, Karen; Meier, Jennie; Charchalac, Ana Paulina

doi:10.1007/s11888-020-00452-5

Cited by 1 publication

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Today, several methods used to treat cancer, but the response to treatment has very weak and often accompanied by undesirable side effects. Therefore, the production of drugs with high efficacy and less toxicity is necessary [1,2]. Many compounds have studied to target cancer cells and the success of these compounds as an anti-cancer agent depend on their ability to activate pathways that cause cancer cells to die [3].…”

Section: Introductionmentioning

confidence: 99%

Allium jesdianum Extract Induces Oxidative Stress and Necroptosis in Human Colorectal Cancer (HT-29) Cell Line

Alidadi

Shirani

Samimi

et al. 2021

Braz. arch. biol. technol.

View full text Add to dashboard Cite

This study aimed to evaluate the toxic impact of hydro-alcoholic Allium jesdianum extract (AJE) on the growth of HT-29 human colorectal cancer cell line. Phytochemical analysis using gas chromatography and mass spectroscopy (GCMS) was done to determine the bioactive components of AJE. HT-29 cells exposed to 0 (control), 25, 50, and 100 g/mL of AJE for 48 hours. Cell survival, colony numbers, flow cytometry, oxidative stress, and gene expression were examined to evaluate the toxic impacts of the AJE. Twelve different phyto-constituents with peak areas were determined by the GCMS analysis. The major compounds were Allicin and α-Pinene. AJE considerably reduced the viability and colony numbers of the HT-29 cells. The AJE concentration-dependently increased necrosis, but not apoptosis in the HT-29 cells. AJE upregulated the expression of necroptosis-associated genes including RIPK1, RIPK3, and MLKL in a concentration-dependent manner. AJE also dose-dependently enhanced MDA contents and reactive oxygen species (ROS) level and diminished antioxidant enzyme level in the HT-29 cells. These data collectively indicated that AJE prevented the growth of the HT-29 cells by inducing oxidative stress, and activation necroptosis signaling pathways. HIGHLIGHTS Allium jesdianum extract inhibits proliferation of HT-29 cells  Allium jesdianum extract induces necroptosis in HT-29 cells  Allium jesdianum extract induces oxidative stress in HT-29 cells 2 Alidadi, H; et al.

show abstract

Section: Introductionmentioning

confidence: 99%