Current progress in bionanomaterials to modulate the epigenome

Rhodes, Anna; Duran-Mota, Jose Antonio; Oliva, Nuria

doi:10.1039/d2bm01027e

Cited by 3 publications

(1 citation statement)

References 83 publications

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“…Pioneering research seeks to improve the interactions between material surfaces and adjacent host (patient) tissues, often referred to as the bioactivity zone [ 20 ]. To this end, materials are designed to provide bioinstructive cues to influence specific cellular signals and responses [ 21 , 22 , 23 ]. This approach has shown some promise in hard tissue repair materials [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Functionalization of Texturized Polypropylene Surface by Silanization and HBII-RGD Attachment on Response of Primary Abdominal and Vaginal Fibroblasts

Quiles,

Rodríguez-Contreras,

Guillem-Marti

et al. 2024

Polymers

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Soft tissue defects, such as incisional hernia or pelvic organ prolapse, are prevalent pathologies characterized by a tissue microenvironment rich in fragile and dysfunctional fibroblasts. Precision medicine could improve their surgical repair, currently based on polymeric materials. Nonetheless, biomaterial-triggered interventions need first a better understanding of the cell-material interfaces that truly consider the patients’ biology. Few tools are available to study the interactions between polymers and dysfunctional soft tissue cells in vitro. Here, we propose polypropylene (PP) as a matrix to create microscale surfaces w/wo functionalization with an HBII-RGD molecule, a fibronectin fragment modified to include an RGD sequence for promoting cell attachment and differentiation. Metal mold surfaces were roughened by shot blasting with aluminum oxide, and polypropylene plates were obtained by injection molding. HBII-RGD was covalently attached by silanization. As a proof of concept, primary abdominal and vaginal wall fasciae fibroblasts from control patients were grown on the new surfaces. Tissue-specific significant differences in cell morphology, early adhesion and cytoskeletal structure were observed. Roughness and biofunctionalization parameters exerted unique and combinatorial effects that need further investigation. We conclude that the proposed model is effective and provides a new framework to inform the design of smart materials for the treatment of clinically compromised tissues.

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