Current prospects for the production of coenzyme Q10 in microbes

Cluis, Corinne P.; Burja, Adam M.; Martin, Vincent J. J.

doi:10.1016/j.tibtech.2007.08.008

Cited by 83 publications

(61 citation statements)

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“…Coenzyme Q 10 (CoQ 10 ) is commercially important nutraceutical compound used extensively in supportive treatment of cardiomyopathy, cancer, neurodegenerative diseases and diseases associated with CoQ 10 deficiency [1,2]. Structurally, it is a benzoquinone with chain of ten isoprene units attached to it.…”

Section: Introductionmentioning

confidence: 99%

“…CoQ 10 level in the body is a measure of health since the low levels are associated with ageing and related diseases causing decreased energy and susceptibility to oxidative stress. The oral formulations containing CoQ 10 have proved to be useful in improving serum CoQ 10 levels and its cosmetic formulations has an anti-ageing property; therefore there is great demand for this molecule in the market [1,4].…”

Section: Introductionmentioning

confidence: 99%

“…The selection of suitable strains and further improving the productivity is prerequisite for cost effective production of CoQ 10 by fermentation [1]. Therefore research efforts on the production of CoQ 10 by microorganisms are focused on the development of potent strains by conventional mutagenesis and metabolic engineering, analysis and modification of the key metabolic pathway, and optimization of fermentation strategies [4]. The E. coli produces CoQ 8 whereas S. cerevisiae produces CoQ 6 ; therefore these well established strains are not suitable for CoQ 10 fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore research efforts on the production of CoQ 10 by microorganisms are focused on the development of potent strains by conventional mutagenesis and metabolic engineering, analysis and modification of the key metabolic pathway, and optimization of fermentation strategies [4]. The E. coli produces CoQ 8 whereas S. cerevisiae produces CoQ 6 ; therefore these well established strains are not suitable for CoQ 10 fermentation. The genetic engineering efforts for CoQ 10 production in E. coli and S. cerevisiae did not give very encouraging results in terms of production yields [1,[6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…The E. coli produces CoQ 8 whereas S. cerevisiae produces CoQ 6 ; therefore these well established strains are not suitable for CoQ 10 fermentation. The genetic engineering efforts for CoQ 10 production in E. coli and S. cerevisiae did not give very encouraging results in terms of production yields [1,[6][7][8][9]. Hence, the commercial process relies on bacterial and yeast strains, which are natural producers of CoQ 10 .…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Differentiation of Sporidiobolus johnsonii ATCC 20490 and Its Coenzyme Q10 Overproducing Mutant Strain UF16

et al. 2014

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Coenzyme Q 10 (CoQ 10 ) is an industrially important molecule having nutraceutical and cosmeceutical applications. CoQ 10 is mainly produced by microbial fermentation and the process demands the use of strains with high productivity and yields of CoQ 10 . During strain improvement program consisting of sequential induced mutagenesis, rational selection and screening process, a mutant strain UF16 was generated from Sporidiobolus johnsonii ATCC 20490 with 2.3-fold improvements in CoQ 10 content. EMS and UV rays were used as mutagenic agents for generating UF16 and it was rationally selected based on atorvastatin resistance as well as survival at free radicals exposure. We investigated the genotypic and phenotypic changes in UF16 in order to differentiate it from wild type strain. Morphologically it was distinct due to reduced pigmentation of colony, reduced cell size and significant reduction in mycelial growth forms with abundance of yeast forms. At molecular level, UF16 was differentiated based on PCR fingerprinting method of RAPD as well as large and small-subunit rRNA gene sequences.Rapid molecular technique of RAPD analysis using six primers showed 34 % polymorphic fragments with mean genetic distance of 0.235. The partial sequences of rRNAgene revealed few mutation sites on nucleotide base pairs. However, the mutations detected on rRNA gene of UF16 were less than 1 % of total base pairs and its sequence showed 99 % homology with the wild type strain. These mutations in UF16 could not be linked to phenotypic or genotypic changes on CoQ 10 biosynthetic pathway that resulted in improved yield. Hence, investigating the mutations responsible for deregulation of CoQ 10 pathway is essential to understand the cause of overproduction in UF16. Phylogenetic analysis based on RAPD bands and rRNA gene sequences coupled with morphological variations, exhibited the novelty of mutant UF16 having potential for improved CoQ 10 production.

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Section: Introductionmentioning

confidence: 99%