Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock

Binsila, Bala Krishnan; Selvaraju, Sellappan; Ranjithkumaran, Rajan; Archana, Santhanahalli Siddalingappa; Balaganur, Krishnappa; Ghosh, Subrata; Kumar, Harendra; Subbarao, Raghavendra Baregundi; Arunachalam, A.; Bhatta, Raghavendra

doi:10.1007/s10815-021-02334-7

Cited by 3 publications

(5 citation statements)

References 205 publications

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“…However, these germ cells were no longer observed in adulthood in the KO testes, while Het testes showed a mix of Sertoli-cell-only tubules and seminiferous tubules with full spermatogenesis (asterisks, Figure 1). To determine an appropriate time point for SSC transplantation, we monitored the presence of germ cells based on the expression of UCH-L1, a marker for gonocytes/early spermatogonia [22,28,34]. While some germ cells were present in 1 wko DAZL-KO pig testes, complete germ cell depletion was observed at 10 weeks of age (10 wko) as well as To determine an appropriate time point for SSC transplantation, we monitored the presence of germ cells based on the expression of UCH-L1, a marker for gonocytes/early spermatogonia [22,28,34].…”

Section: Composition Of the Testicular Parenchyma In Dazl-ko Pigsmentioning

confidence: 99%

“…To determine an appropriate time point for SSC transplantation, we monitored the presence of germ cells based on the expression of UCH-L1, a marker for gonocytes/early spermatogonia [22,28,34]. While some germ cells were present in 1 wko DAZL-KO pig testes, complete germ cell depletion was observed at 10 weeks of age (10 wko) as well as To determine an appropriate time point for SSC transplantation, we monitored the presence of germ cells based on the expression of UCH-L1, a marker for gonocytes/early spermatogonia [22,28,34]. While some germ cells were present in 1 wko DAZL-KO pig testes, complete germ cell depletion was observed at 10 weeks of age (10 wko) as well as in adult testes (Figure 2).…”

Section: Composition Of the Testicular Parenchyma In Dazl-ko Pigsmentioning

confidence: 99%

“…Therefore, SSC transplantation could serve to accelerate biomedical research, enable efficient propagation of valuable models, and rescue difficult-to-breed genotypes, besides contributing to advancements in human health and the development of novel treatments and therapies [5,25,26]. However, optimization of the transplantation techniques is still ongoing, especially in large animals, where improvement of SSC expansion and survival in vitro, colonization of the recipient testes by transplanted cells, and long-term functionality of the transplanted SSCs are research goals [2,5,7,14,27,28].…”

Section: Introductionmentioning

confidence: 99%

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DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation

Lara,

Goldsmith,

Rodriguez-Villamil

et al. 2023

Cells

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Spermatogonial stem cell (SSC) transplantation into the testis of a germ cell (GC)-depleted surrogate allows transmission of donor genotype via donor-derived sperm produced by the recipient. Transplantation of gene-edited SSCs provides an approach to propagate gene-edited large animal models. DAZL is a conserved RNA-binding protein important for GC development, and DAZL knockout (KO) causes defects in GC commitment and differentiation. We characterized DAZL-KO pigs as SSC transplantation recipients. While there were GCs in 1-week-old (wko) KO, complete GC depletion was observed by 10 wko. Donor GCs were transplanted into 18 DAZL-KO recipients at 10–13 wko. At sexual maturity, semen and testes were evaluated for transplantation efficiency and spermatogenesis. Approximately 22% of recipient seminiferous tubules contained GCs, including elongated spermatids and proliferating spermatogonia. The ejaculate of 89% of recipients contained sperm, exclusively from donor origin. However, sperm concentration was lower than the wild-type range. Testicular protein expression and serum hormonal levels were comparable between DAZL-KO and wild-type. Intratesticular testosterone and Leydig cell volume were increased, and Leydig cell number decreased in transplanted DAZL-KO testis compared to wild-type. In summary, DAZL-KO pigs support donor-derived spermatogenesis following SSC transplantation, but low spermatogenic efficiency currently limits their use for the production of offspring.

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Section: Composition Of the Testicular Parenchyma In Dazl-ko Pigsmentioning

confidence: 99%

Section: Composition Of the Testicular Parenchyma In Dazl-ko Pigsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation

Lara,

Goldsmith,

Rodriguez-Villamil

et al. 2023

Cells

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“…Some of the most prominent approaches to preserve male fertility include sperm and testicular tissue cryopreservation; germ cells transplantation, and testicular grafts ( 31 – 34 ). An efficient germ cell maintenance is essential for in vitro fertilization as for intracytoplasmic sperm injection (ICSI), which is one of the most well-succeeded techniques in assisted reproduction ( 35 , 36 ). When the spermatozoid production is not possible, the use of spermatogonial stem cells (SSCs) is considered a viable option ( 35 ), but due to several experimental difficulties as stem cells isolation, identification, purification and in vitro maintenance, this approach remains limited ( 36 ).…”

Section: Introductionmentioning

confidence: 99%

“…An efficient germ cell maintenance is essential for in vitro fertilization as for intracytoplasmic sperm injection (ICSI), which is one of the most well-succeeded techniques in assisted reproduction ( 35 , 36 ). When the spermatozoid production is not possible, the use of spermatogonial stem cells (SSCs) is considered a viable option ( 35 ), but due to several experimental difficulties as stem cells isolation, identification, purification and in vitro maintenance, this approach remains limited ( 36 ). Although its broad potential in the reproduction field, its effectiveness was reported in rodents, remaining a challenge in larger species ( 37 , 38 ).…”

Section: Introductionmentioning

confidence: 99%

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

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Traditional therapeutic interventions aim to restore male fertile potential or preserve sperm viability in severe cases, such as semen cryopreservation, testicular tissue, germ cell transplantation and testicular graft. However, these techniques demonstrate several methodological, clinical, and biological limitations, that impact in their results. In this scenario, reproductive medicine has sought biotechnological alternatives applied for infertility treatment, or to improve gamete preservation and thus increase reproductive rates in vitro and in vivo. One of the main approaches employed is the biomimetic testicular tissue reconstruction, which uses tissue-engineering principles and methodologies. This strategy pursues to mimic the testicular microenvironment, simulating physiological conditions. Such approach allows male gametes maintenance in culture or produce viable grafts that can be transplanted and restore reproductive functions. In this context, the application of several biomaterials have been proposed to be used in artificial biological systems. From synthetic polymers to decellularized matrixes, each biomaterial has advantages and disadvantages regarding its application in cell culture and tissue reconstruction. Therefore, the present review aims to list the progress that has been made and the continued challenges facing testicular regenerative medicine and the preservation of male reproductive capacity, based on the development of tissue bioengineering approaches for testicular tissue microenvironment reconstruction.

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