Current Studies on a New �C�-Type Virus of Presumed Reptilian Origin

Rf, Zeigel; Hf, Clark

doi:10.1159/000391766

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To increase binding of partially degradated RNA to oligo(dT) 25 Dynabeads (Dynal), nucleic acids from 20 l of sucrose peak fraction were polyadenylated for 20 min at 37°C in 40 l of reaction mixture containing 50 mM Tris-HCl (pH 7.9), 250 mM NaCl, 10 mM MgCl 2 , 2.5 mM MnCl 2 , 10 mM dithiothreitol (DTT), 40 U of RNasin (Promega), 0.15% Nonidet P-40 (NP-40), 100 M ATP, 4 U of poly(A) polymerase (Gibco BRL), and 50 g of oligo(dT) 25 Dynabeads.…”

Section: Methodsmentioning

confidence: 99%

“…Retroviruses were identified in snakes more than 30 years ago, when C-type-like particles were observed by electron microscopy in the tissues of a tumor-bearing viper (25)(26)(27) and were later shown to be related to primate D-type retroviruses (1). Retrovirus-like particles subsequently detected in snakes also involved mostly animals affected with proliferative disorders (12,14,17) but were also found in normal tissues (6).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of Two Closely Related Unclassifiable Endogenous Retroviruses in Pythons (Python molurusandPython curtus)

Huder

Böni

Hatt³

et al. 2002

J Virol

View full text Add to dashboard Cite

Boid inclusion body disease (BIBD) is a fatal disorder of boid snakes that is suspected to be caused by a retrovirus. In order to identify this agent, leukocyte cultures (established from Python molurus specimens with symptoms of BIBD or kept together with such diseased animals) were assessed for reverse transcriptase (RT) activity. Virus from cultures exhibiting high RT activity was banded on sucrose density gradients, and the RT peak fraction was subjected to highly efficient procedures for the identification of unknown particle-associated retroviral RNA. A 7-kb full retroviral sequence was identified, cloned, and sequenced. This virus contained intact open reading frames (ORFs) for gag, pro, pol, and env, as well as another ORF of unknown function within pol. Phylogenetic analysis showed that the virus is distantly related to viruses from both the B and D types and the mammalian C type but cannot be classified. It is present as a highly expressed endogenous retrovirus in all P. molurus individuals; a closely related, but much less expressed virus was found in all tested Python curtus individuals. All other boid snakes tested, including Python regius, Python reticulatus, Boa constrictor, Eunectes notaeus, and Morelia spilota, were virus negative, independent of whether they had BIBD or not. Virus isolated from P. molurus could not be transmitted to the peripheral blood mononuclear cells of B. constrictor or P. regius. Thus, there is no indication that this novel virus, which we propose to name python endogenous retrovirus (PyERV), is causally linked with BIBD.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%