Current Trends in Magnetic Particle Enrichment for Mass Spectrometry-Based Analysis of Cardiovascular Protein Biomarkers

Schneck, Nicole A.; Lowenthal, Mark S.; Phinney, Karen W.; Lee, Sang Bok

doi:10.2217/nnm.14.188

Cited by 26 publications

(9 citation statements)

References 87 publications

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“…Initially we are using targeted MS with immunoprecipitation. In parallel, dual binders enzyme-linked immunosorbent assay (ELISA) is developed to enable quantification of the protein in plasma (78,79). This enables validation of PDGFB with estimation of the sensitivity and specificity for disease status.…”

Section: Discussionmentioning

confidence: 99%

F11 is associated with recurrent VTE in women

Bruzelius¹,

Ljungqvist²,

Bottai³

et al. 2016

Thromb Haemost

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Genetic associations for the reoccurrence of venous thromboembolism (VTE) are not well described. Our aim was to investigate if common genetic variants, previously found to contribute to the prediction of first time thrombosis in women, were associated with risk of recurrence. The Thromboembolism Hormone Study (TEHS) is a Swedish nationwide case-control study (2002-2009). A cohort of 1,010 women with first time VTE was followed up until a recurrent event, death or November 2011. The genetic variants in F5 rs6025, F2 rs1799963, ABO rs514659, FGG rs2066865, F11 rs2289252, PROC rs1799810 and KNG1 rs710446 were assessed together with clinical variables. Recurrence rate was calculated as the number of events over the accumulated patient-time. Cumulative recurrence was calculated by Kaplan-Meier curve. Cox proportional-hazard model was used to estimate hazard ratios (HR) and 95% confidence intervals (95% CI) between groups. A total of 101 recurrent events occurred during a mean follow-up time of five years. The overall recurrence rate was 20 per 1,000 person-years (95% CI; 16-24). The recurrence rate was highest in women with unprovoked first event and obesity. Carriers of the risk alleles of F5 rs6025 (HR=1.7 (95% CI; 1.1-2.6)) and F11 rs2289252 (HR=1.8 (95% CI; 1.1-3.0)) had significantly higher rates of recurrence compared to non-carriers. The cumulative recurrence was 2.5-fold larger in carriers of both F5 rs6025 and F11 rs2289252 than in non-carriers at five years follow-up. In conclusion, F5 rs6025 and F11 rs2289252 contributed to the risk of recurrent VTE and the combination is of potential clinical relevance for risk prediction.

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Section: Discussionmentioning

confidence: 99%

F11 is associated with recurrent VTE in women

Bruzelius¹,

Ljungqvist²,

Bottai³

et al. 2016

Thromb Haemost

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“…Although not directly associated with cell viability, these results indicate maintenance of EC survival under hypergravity conditions. Moreover, in this work, the effect of hypergravity in EC morphology was assessed as previously described [21]. Although hypergravity has been suggested to affect cell morphology, particularly for mesenchymal stem cells cultured at 20g for 3 h [21], no major alterations were observed in this work.…”

Section: Endothelial Cell Morphology and Cytoskeleton Organization Unmentioning

confidence: 91%

“…Moreover, in this work, the effect of hypergravity in EC morphology was assessed as previously described [21]. Although hypergravity has been suggested to affect cell morphology, particularly for mesenchymal stem cells cultured at 20g for 3 h [21], no major alterations were observed in this work. These results suggest that morphological adaptation upon hypergravity exposure may be dependent on distinct parameters, including cell type and g-level.…”

Section: Endothelial Cell Morphology and Cytoskeleton Organization Unmentioning

confidence: 91%

“…All antibodies were diluted in 1% (w/v) bovine serum Cell morphology was then evaluated in a minimum of 50 cells per condition using IMAGEJ software (http://rsb.info.nih.gov/ij). Cells were evaluated in terms of area, circularity, solidity and roundness as described elsewhere [21]: (i) circularity ¼ 4p area perimeter 22 , where area and perimeter are the area and perimeter of each cell; (ii) solidity ¼ area/convex area, where convex area is the area of the smallest convex polygon containing the cell; and (iii) roundness ¼ width length 21 of the smallest rectangle enclosing the cell.…”

Section: Characterization Of Cell Behaviour 241 Cytoskeleton Organization By Immunofluorescence and Cell Morphology Analysismentioning

confidence: 99%

Section: Endothelial Cell Morphology and Cytoskeleton Organization Under Hypergravitymentioning

confidence: 99%

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Effects of hypergravity on the angiogenic potential of endothelial cells

Costa‐Almeida

Carvalho

Ferreira

et al. 2016

J. R. Soc. Interface.

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Angiogenesis, the formation of blood vessels from pre-existing ones, is a key event in pathology, including cancer progression, but also in homeostasis and regeneration. As the phenotype of endothelial cells (ECs) is continuously regulated by local biomechanical forces, studying endothelial behaviour in altered gravity might contribute to new insights towards angiogenesis modulation. This study aimed at characterizing EC behaviour after hypergravity exposure (more than 1g), with special focus on cytoskeleton architecture and capillary-like structure formation. Herein, human umbilical vein ECs (HUVECs) were cultured under two-dimensional and three-dimensional conditions at 3g and 10g for 4 and 16 h inside the large diameter centrifuge at the European Space Research and Technology Centre (ESTEC) of the European Space Agency. Although no significant tendency regarding cytoskeleton organization was observed for cells exposed to high g's, a slight loss of the perinuclear localization of b-tubulin was observed for cells exposed to 3g with less pronounced peripheral bodies of actin when compared with 1g control cells. Additionally, hypergravity exposure decreased the assembly of HUVECs into capillary-like structures, with a 10g level significantly reducing their organization capacity. In conclusion, short-term hypergravity seems to affect EC phenotype and their angiogenic potential in a time and g-level-dependent manner.

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Effects of Graphene Quantum Dots on the Self‐Renewal and Differentiation of Mesenchymal Stem Cells

Qiu

Mou

et al. 2016

Adv Healthcare Materials

112

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The influence of graphene quantum dots (GQDs) on key characteristics of bone marrow derived mesenchymal stem cells (MSCs) phenotype (i.e., self-renewal, differentiation potential, and pluripotency) is systematically investigated in this work. First, the viability and impact of GQDs on the self-renewal potential of MSCs is evaluated in order to determine a threshold for the exposing dose. Second, GQDs uptake by MSCs is confirmed due to the excellent fluorescent properties of the particles. They exhibit a homogenous cytoplasmatic distribution that increases with the time and concentration. Third, the impact of GQDs on the osteogenic differentiation of MSCs is deeply characterized. An enhanced activity of alkaline phosphatase promoted by GQDs indicates early activation of osteogenesis. This is also confirmed upon GQD-induced up-regulation of phenotypically related osteogenic genes (Runx2, osteopontin, and osteocalcin) and specific biomarkers expression (osteopontin and osteocalcin). GQDs also effectively enhance the formation of calcium-rich deposits characteristics of osteoblasts. Furthermore, genes microarray results indicate that the enhanced osteogenic differentiation of MSCs by GQDs is in progress through a bone morphogenetic protein and transforming growth factor-β relative signaling pathways. Finally, intracytoplasmatic lipid detection shows that GQDs can also promote the adipogenic differentiation of MSCs, thus confirming the prevalence of their pluripotency potential.

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Current Trends in Magnetic Particle Enrichment for Mass Spectrometry-Based Analysis of Cardiovascular Protein Biomarkers

Cited by 26 publications

References 87 publications

F11 is associated with recurrent VTE in women

F11 is associated with recurrent VTE in women

Effects of hypergravity on the angiogenic potential of endothelial cells

Effects of Graphene Quantum Dots on the Self‐Renewal and Differentiation of Mesenchymal Stem Cells

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