Custom-made rheological setup for in situ real-time fast alginate-Ca2+ gelation

Besiri, Ioanna N.; Goudoulas, Thomas B.

doi:10.1016/j.carbpol.2020.116615

Cited by 16 publications

(15 citation statements)

References 37 publications

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“…For injectable hydrogels, the change in ambient temperature − or pH ,, in the body may be exploited to mechanically strengthen hydrogels. While the temperature can be readily adapted in most rheological setups, the variation of pH or ionic strength may require specialized geometries that allow for subphase exchange, controlled setting of hydrogels, or the use of slow-dissolving salts or acids . Magnetic hydrogels have been developed and investigated using specialized rheological setups, allowing the local heating or stiffening upon application of a magnetic or electrical field. , A common stimulus-responsive reinforcement strategy that is particularly useful for 3D printing inks involves the application of photo-cross-linking after extrusion, which requires a transparent UV-rheology setup to capture the increase in mechanical properties. , …”

Section: Rheology Guide To Self-healing Injectable Hydrogelsmentioning

confidence: 99%

“…For injectable hydrogels, the change in ambient temperature 285−292 or pH 61,98,99 in the body may be exploited to mechanically strengthen hydrogels. While the temperature can be readily adapted in most rheological setups, the variation of pH or ionic strength may require specialized geometries that allow for subphase exchange, 293 controlled setting of hydrogels, 294 or the use of slow-dissolving salts or acids. 61 Magnetic hydrogels have been developed and investigated using specialized rheological setups, allowing the local heating or stiffening upon application of a magnetic or electrical field.…”

Section: Measuring Self-healing and Stimulus-responsementioning

confidence: 99%

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Self-Healing Injectable Hydrogels for Tissue Regeneration

et al. 2022

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Biomaterials with the ability to self-heal and recover their structural integrity offer many advantages for applications in biomedicine. The past decade has witnessed the rapid emergence of a new class of self-healing biomaterials commonly termed injectable, or printable in the context of 3D printing. These self-healing injectable biomaterials, mostly hydrogels and other soft condensed matter based on reversible chemistry, are able to temporarily fluidize under shear stress and subsequently recover their original mechanical properties. Self-healing injectable hydrogels offer distinct advantages compared to traditional biomaterials. Most notably, they can be administered in a locally targeted and minimally invasive manner through a narrow syringe without the need for invasive surgery. Their moldability allows for a patient-specific intervention and shows great prospects for personalized medicine. Injected hydrogels can facilitate tissue regeneration in multiple ways owing to their viscoelastic and diffusive nature, ranging from simple mechanical support, spatiotemporally controlled delivery of cells or therapeutics, to local recruitment and modulation of host cells to promote tissue regeneration. Consequently, self-healing injectable hydrogels have been at the forefront of many cutting-edge tissue regeneration strategies. This study provides a critical review of the current state of self-healing injectable hydrogels for tissue regeneration. As key challenges toward further maturation of this exciting research field, we identify (i) the trade-off between the self-healing and injectability of hydrogels vs their physical stability, (ii) the lack of consensus on rheological characterization and quantitative benchmarks for self-healing injectable hydrogels, particularly regarding the capillary flow in syringes, and (iii) practical limitations regarding translation toward therapeutically effective formulations for regeneration of specific tissues. Hence, here we (i) review chemical and physical design strategies for self-healing injectable hydrogels, (ii) provide a practical guide for their rheological analysis, and (iii) showcase their applicability for regeneration of various tissues and 3D printing of complex tissues and organoids.

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Section: Rheology Guide To Self-healing Injectable Hydrogelsmentioning

confidence: 99%

Section: Measuring Self-healing and Stimulus-responsementioning

confidence: 99%

Self-Healing Injectable Hydrogels for Tissue Regeneration

et al. 2022

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“…In the latest research studies, a custom-made rheometric setup is developed for real-time recording of fast crosslinking reactions. 15,28 It consists of two parts that provide an inner cavity when firmly coupled. A volumetric syringe is connected to a side-feed hole, where the crosslinking solution is applied.…”

Section: Custom-made Rheometric Setupmentioning

confidence: 99%

In situ evaluation of alginate‐Ca²⁺ gelation kinetics

Besiri

Goudoulas

Fattahi

et al. 2023

J of Applied Polymer Sci

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A thorough assessment of gelation progress requires real-time recording of biopolymer kinetics. In this study, an in situ investigation with a customized parallel-plate rheometric setup examines how the initial distribution of CaCl 2 to alginate through micro-holes affects the process. At constant volume and concentration of reactants, stiffer gels are produced at the same probing time by increasing the number of suppliers (2-12). Due to the oscillation and the different local distribution of the cations into the biopolymer solution, the development of gel front is affected, which is for the first time in situ rheologically investigated by the temporal evaluation of complex modulus, G*. The alternative configurations of the same number of micro-holes have drastically contribution to this regard, too. Moreover, a two-kernel equation is introduced predicting the alginate-Ca 2+ gelation kinetics. It represents the initial fast increase in G*, determined by the concentration of cross-linker, and the slow diffusion of cations to the assembled gelling structures depicted on the successive evolution of G* in longer times. Finally, a continuous and an intermittent oscillatory time sweep influence the diffusion of the cross-linking solution resulting in considerably different gelation curves for the same concentration of reactants.

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“…A new custom-made rheometric setup was able to record the fast response from the very beginning, thus both the concentration and volume of the crosslinker could be controlled. 84 Again, injectable self crosslinking property was introduced through reaction between alginated dialdehyde and gelatin. 85…”

Section: Alginate Based Gelmentioning

confidence: 99%

Recent Development of Polysaccharide-Derived Hydrogel: Properties, Stimuli-Responsiveness and Bioapplications

Mukherjee

2022

Preprint

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Hydrogels are three-dimensional, hydrophilic, polymeric frameworks, constructed through diverse physical or chemical crosslinks. Recent research focuses on their physiological stimuli sensitivity enhancing bioapplications. Biopolymers with responsiveness to local environment like temperature, pH etc. form the foundation in this regard, around which all major natural processes are circulated. Polysaccharides, being most the abundant biopolymers and essential constituent of our daily food like cereals and fruits, are readily available in nature. Hence, among numerous biomacromolecules, polysaccharides are extensively used to prepare hydrogel recently. However, incorporation of certain properties to these gels through derivatization can trigger stimuli responsiveness suitable for various applications especially in biomedical field inspired from the mother nature. For example, in situ cross-linking ability may deliver an intermediate platform between solid scaffolds and saline injections through reversible or irreversible sol-gel transition. Easy injectability provides patient’s relief through reducing recovery time and inflectional hazards. Self recovery of gel within body results automatic inflammation and damage recovery of tissues. This review provides a detail impression on the synthesis of natural polysaccharide derived hydrogel network with structural classification to bulk, supramolecular, micro and nano gel. Several properties important from biomedical point such as in situ, injectable, self healing nature of these hydrogels and various physical, chemical, or biochemical stimuli-responsive characteristics are discussed further. Lastly, their major bioapplications are highlighted.

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Custom-made rheological setup for in situ real-time fast alginate-Ca2+ gelation

Cited by 16 publications

References 37 publications

Self-Healing Injectable Hydrogels for Tissue Regeneration

Self-Healing Injectable Hydrogels for Tissue Regeneration

In situ evaluation of alginate‐Ca²⁺ gelation kinetics

Recent Development of Polysaccharide-Derived Hydrogel: Properties, Stimuli-Responsiveness and Bioapplications

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Custom-made rheological setup for in situ real-time fast alginate-Ca2+ gelation

Cited by 16 publications

References 37 publications

Self-Healing Injectable Hydrogels for Tissue Regeneration

Self-Healing Injectable Hydrogels for Tissue Regeneration

In situ evaluation of alginate‐Ca2+ gelation kinetics

Recent Development of Polysaccharide-Derived Hydrogel: Properties, Stimuli-Responsiveness and Bioapplications

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Product

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In situ evaluation of alginate‐Ca²⁺ gelation kinetics