Customizable and stable multilocus chromosomal integration: a novel glucose-dependent selection system in Aureobasidium spp.
Shuo Zhang,
Tao Ma,
Fu-Hui Zheng
et al.
Abstract:Background
Non-conventional yeasts hold significant potential as biorefinery cell factories for microbial bioproduction. Currently, gene editing systems used for these yeasts rely on antibiotic and auxotrophic selection mechanisms. However, the drawbacks of antibiotics, including high costs, environmental concerns, and the dissemination of resistance genes, make them unsuitable for large-scale industrial fermentation. For auxotrophic selection system, the engineered strains harboring auxotrophi… Show more
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