Cutaneous Wound Healing

Singer, Adam J.; Clark, Richard A.F.

doi:10.1056/nejm199909023411006

Cited by 5,343 publications

(4,963 citation statements)

References 67 publications

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“…Plasma containing platelets and leukocytes undergo spontaneous coagulation. Like in the natural blood clot that forms at the defect site, activated platelets and leukocytes are entrapped in the fibrin‐rich matrix (Singer & Clark, 1999). PRF can be further processed, for example, by squeezing out further serum resulting in a PRF membrane (Dohan et al., 2006) or by mixing with grafts and biomaterials as summarized in this and other reviews.…”

Section: Introductionmentioning

confidence: 99%

The use of platelet‐rich fibrin to enhance the outcomes of implant therapy: A systematic review

Strauß

Stähli

Gruber

2018

Clinical Oral Implants Res

105

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ObjectiveTo assess the impact of platelet‐rich fibrin (PRF) on implant dentistry. The primary focused question was as follows: What are the clinical, histological, and radiographic outcomes of PRF administration for bone regeneration and implant therapy?MethodA systematic literature search comprised three databases: MEDLINE, EMBASE, and Cochrane followed by a hand search of relevant scientific journals. Human studies using PRF for bone regeneration and implant therapy were considered and articles published up to December 31, 2017 were included. Eligible studies were selected based on the inclusion criteria. Randomized controlled trials (RCT) and controlled clinical trials (CCT) were included.ResultsIn total, 5,963 titles were identified with the search terms and by hand search. A total of 12 randomized controlled trials (RCT) met the inclusion criteria and were chosen for data extraction. Included studies focused on alveolar ridge preservation after tooth extraction, osseointegration process, soft tissue management, bone augmentation, bone regeneration after sinus floor elevation and surgical peri‐implantitis treatment. Overall, the risk of bias was moderate or unclear. Nine studies showed superior outcomes for PRF for any of the evaluated variables, such as ridge dimension, bone regeneration, osseointegration process, soft tissue healing. Three studies failed to show any beneficial effects of PRF. No meta‐analysis could be performed due to the heterogeneity of study designs.ConclusionsThere is moderate evidence supporting the clinical benefit of PRF on ridge preservation and in the early phase of osseointegration. It remains unclear whether PRF can reduce pain and improve soft tissue healing. More research support is necessary to comment on the role of PRF to improve other implant therapy outcomes.

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Section: Introductionmentioning

confidence: 99%

The use of platelet‐rich fibrin to enhance the outcomes of implant therapy: A systematic review

Strauß

Stähli

Gruber

2018

Clinical Oral Implants Res

105

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“…11,16 Wound healing is a complex, dynamic process in which angiogenesis has been hypothesized to play an integral part since the formation of new blood vessels is a necessary step that allows a variety of mediators and regulators to reach the center of the healing process. 17 VEGF appears to play a crucial role during the proliferative phase of wound healing. VEGF levels measured in wound fluids from surgical wounds increase postoperatively, and harvested wound fluid induces a neovascular response in the rat corneal micropocket assay that is significantly attenuated by VEGF neutralization.…”

Section: Introductionmentioning

confidence: 99%

Discordant effects of a soluble VEGF receptor on wound healing and angiogenesis

et al. 2004

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Soluble receptors to vascular endothelial growth factor (VEGF) can inhibit its angiogenic effect. Since angiogenesis is involved in wound repair, we hypothesized that adenovirus-mediated gene transfer of a soluble form of VEGF receptor 2 (Flk-1) would attenuate wound healing in mice. C57Bl/6J and genetically diabetic (db/db) mice (each n¼20) received intravenous (i.v.) injections of recombinant adenoviruses (10 9 PFU) encoding the ligand-binding ectodomain of VEGF receptor 2 (Flk-1) or cDNA encoding the murine IgG2a Fc fragment (each n¼10). At 4 days after gene transfer, two full-thickness skin wounds (0.8 cm) were created on the dorsum of each animal. Wound closure was measured over 9-14 days after which wounds were resected for histological analysis. Prior to killing, fluorescent microspheres were systemically injected for quantitation of wound vascularity. Single i.v. injections of adenoviruses encoding soluble Flk-1 significantly decreased wound angiogenesis in both wild-type and diabetic mice. Fluorescence microscopy revealed a 2.0-fold (wild type) and 2.9-fold (diabetic) reduction in wound vascularity in Flk-1-treated animals (po0.05). Impairment of angiogenesis was confirmed by CD31 immunohistochemistry. Interestingly, despite significant reductions in wound vascularity, wound closure was not grossly delayed. Our data indicates that while VEGF function is essential for optimal wound angiogenesis, it is not required for wound closure.

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“…Growth factors have been shown to influence epidermal and dermal regeneration. 8,9 However, the clinical use of recombinant growth factors has not been as beneficial as hoped. This is not really surprising given the complexity and variability of the interactions between cells, soluble cytokines, blood elements and extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6][7] Wound healing is a dynamic interactive and complex process involving soluble mediators, blood cells, extracellular matrix and parenchymal cells. 8 Different cell types synthesize and release different mediators that modulate and stimulate the cascade of wound healing. These mediators encompass multiple chemokines and growth factors.…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, growth factors have been suggested to influence and modulate epidermal and dermal regeneration. 8,9 We have recently shown that liposomal cDNA gene transfer of growth factors is a possible approach to improve dermal and epidermal regeneration. 5,6,10 Hence, liposomes appear to be an effective vector for gene transfer in the skin.…”

Section: Introductionmentioning

confidence: 99%

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The structure and composition of liposomes can affect skin regeneration, morphology and growth factor expression in acute wounds

et al. 2005

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Liposomal gene transfer is an effective therapeutic approach to improve dermal and epidermal regeneration. The purpose of the present study was to define whether the biological or chemical structure of a liposome influences cellular and biological regeneration in the skin, and to determine by which mechanisms possible changes occur. Rats were inflicted a full-excision acute wound and divided into three groups to receive weekly subcutaneous injections of DMRIE liposomes plus the Lac Z gene, or DOTAP/Chol liposomes plus the Lac Z gene, or saline. Planimetry, immunological assays, histological and immunohistochemical techniques were used to determine cellular responses after gene transfer, protein expression, dermal and epidermal regeneration. DOTAP/ Chol increased IGF-I and KGF protein concentration and caused concomitant cellular responses, for example, by increasing IGFBP-3, Po0.05. DOTAP/Chol liposomes improved epidermal regeneration by exhibiting the most rapid area and linear wound re-epithelization compared to DMRIE or control, Po0.001. DOTAP/Chol and DMRIE exerted promitogenic and antiapoptotic effects on basal keratinocytes, Po0.05. Dermal regeneration was improved in DOTAP/Chol-treated animals by an increased collagen deposition and morphology, Po0.001. DOTAP/Chol liposomes increased vascular endothelial growth factor concentrations and thus neovascularization when compared with DMRIE and saline, Po0.001. In the present study, we showed that different liposomes have different effects on intracellular and biological responses based on its chemical and molecular structure. For gene transfer in acute wounds, the administration of DOTAP/Chol liposomes appears to be beneficial.

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Cutaneous Wound Healing

Cited by 5,343 publications

References 67 publications

The use of platelet‐rich fibrin to enhance the outcomes of implant therapy: A systematic review

The use of platelet‐rich fibrin to enhance the outcomes of implant therapy: A systematic review

Discordant effects of a soluble VEGF receptor on wound healing and angiogenesis

The structure and composition of liposomes can affect skin regeneration, morphology and growth factor expression in acute wounds

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