Cutting Edge: The Mouse NK Cell-Associated Antigen Recognized by DX5 Moncoclonal Antibody is CD49b (α2 Integrin, Very Late Antigen-2)

Abstract

DX5 mAb is a useful reagent because it stains NK cells from all mouse strains examined. We have identified the molecule recognized by DX5 mAb by using a retrovirus-mediated expression cloning system. A 5-kb cDNA encoding a protein that is reactive with the DX5 mAb was isolated from a NK cell cDNA library, and this molecule was identical with CD49b (very late Ag-2, α2 integrin). The DX5 mAb reacted with transfectants expressing CD49b, and binding of DX5 to the NK cells and CD49b transfectants was blocked in the presence of other anti-CD49b mAbs. When NK1.1+ NK cells were cultured with IL-2, they progressively lost reactivity with DX5 mAb as a consequence of cellular proliferation. Cytotoxicity mediated by the DX5+ NK cells was dramatically higher as compared with DX5− NK cells. Therefore, DX5 mAb recognizes CD49b and can be used to define functionally distinct subsets of NK cells.

NK cells activated by Interleukin-4 in cooperation with Interleukin-15 exhibit distinctive characteristics

“…Thus, it might be assumed that IL4-NK cells are immature NK cells. However, IL4-NK cells highly express CD49b, which is expressed on mature NK cells, and do not express TRAIL and IL-7Rα, which are expressed on immature NK cells (16,33,34 contribute to the development of IL4-NK cells. Of note, the present study demonstrates that these unique NK cells are induced and increased in vivo by IL-4.…”

Reduced Stathmin-1 Expression in Natural Killer Cells Associated with Spontaneous Abortion

“…Subsequently, NK cells were isolated using magnetic bead-conjugated antimouse CD49b monoclonal antibody, and CD49b ϩ cells were purified by means of Mini MACS columns (Miltenyi Biotec Inc., Auburn, CA), 11,12 where CD49b was used as a common marker for murine NK cells. 14 The purity of the MACSisolated NK cells routinely exceeded 95% as determined using flow cytometry. 12,15 …”

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

“…IL-12 is one of the major cytokines produced by these cells [18]. A monoclonal antibody specific to CD49b (DX5) is a typical antibody toward pan-NK cells [19]. IFN-γ is one of the major cytokines produced by NK cells and type 1-helper T (Th1) cells [20].…”

“…IFN-γ is one of the major cytokines produced by NK cells and type 1-helper T (Th1) cells [20]. [19,24]. In addition, there are two distinct types of DC precursors: myeloid monocytes (pre-DC1) and plasmacytoid DC precursors (pre-DC2).…”