Cuvette carryover with the gentamicin assay on the Beckman AU480 analyser

Dimeski, Goce; Johnston, Julie; Bassett, Kendra; Shaw, Jacqueline A; Brown, Nigel N.

doi:10.1515/cclm-2015-0187

Cited by 1 publication

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“…One other case of gentamicin interference has recently been described. Dimenski et al 36 observed that internal quality-control data showed intermittent spikes with the gentamicin assay. They conducted a study to determine if reagents for the Beckman AU480 analyzer (Beckman Coulter, Brea, California) contained gentamicin as an antibacterial agent and if it could cause carryover contamination of cuvettes in the instrument.…”

Section: Discussionmentioning

confidence: 99%

Gentamicin and Vancomycin Interference on Results of Clinical Chemistry Parameters on Abbott Architect c8000

Brenčić¹,

Nikolac²

2019

Archives of Pathology &Amp; Laboratory Medicine

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Context.— Gentamicin and vancomycin are nephrotoxic antibiotics. Little is known about the influence of drug concentrations on results of clinical chemistry tests. Objective.— To investigate gentamicin and vancomycin interference on results of 33 commonly measured biochemistry tests. Design.— The study was carried out in the University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice (Zagreb, Croatia). For each drug, 10 aliquots of pooled serum were prepared. In order to cover toxic concentrations, pool serum samples were spiked with drugs to obtain 0 to 50 μg/mL of gentamicin and 0 to 200 μg/mL of vancomycin. Biochemistry tests were measured in duplicate on the Architect c8000 analyzer, and drug concentrations were measured on Architect i2000 SR (both Abbott Laboratories, Abbott Park, Illinois). For each tested concentration, bias was calculated against the initial measurement. Acceptance criteria were defined as measurement uncertainty of the commercial control with the value close to the measured range of the pool sample. Results.— For gentamicin, all bias values were below established criteria. For vancomycin, significant changes were observed for potassium, direct bilirubin, and immunoglobulin A. Significant bias was already detected at low vancomycin concentration (2.98 μg/mL) for direct bilirubin (bias = 9.7%; acceptable = 8%). Potassium bias at the highest vancomycin concentration (204.4 μg/mL) exceeded acceptance criteria (bias = 4.5%; acceptable = 4%). For immunoglobulin A, no apparent trend was observed, and bias is attributed to increased method imprecision. Conclusions.— Gentamicin did not interfere with the results of clinical chemistry tests. Direct bilirubin concentration is falsely increased in the presence of vancomycin, and potassium is affected at high concentrations.

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Section: Discussionmentioning

confidence: 99%