Cux1+ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics

Huang, Minhua; Ning, Hua; Zhuang, Siyi; Fang, Qiuyuan; Shang, Jiangming; Wang, Zhen; Tao, Xiaohua; Niu, Junfeng; Li, Xiang-Yao; Yu, Peilin; Yang, Wei

doi:10.1016/j.jpha.2023.04.004

Cited by 3 publications

(4 citation statements)

References 63 publications

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“…However, based on the DEGs of scButterfly-C, seven out of the top ten significantly enriched biological processes were related to basal cells, especially for morphogenesis, development, and cell cycle. Previous studies indicated that basal cells may contain a subtype named proliferative basal cells (PBC), which usually highly express the genes associated with the positive regulation of the cell cycle 30 , 31 . In non-proliferative basal cells, Krt14 is usually highly expressed, while PBC often shows higher expression of Mki67 and Top2a .…”

Section: Resultsmentioning

confidence: 99%

scButterfly: a versatile single-cell cross-modality translation method via dual-aligned variational autoencoders

Cao,

Zhao,

Tang

et al. 2024

Nat Commun

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Recent advancements for simultaneously profiling multi-omics modalities within individual cells have enabled the interrogation of cellular heterogeneity and molecular hierarchy. However, technical limitations lead to highly noisy multi-modal data and substantial costs. Although computational methods have been proposed to translate single-cell data across modalities, broad applications of the methods still remain impeded by formidable challenges. Here, we propose scButterfly, a versatile single-cell cross-modality translation method based on dual-aligned variational autoencoders and data augmentation schemes. With comprehensive experiments on multiple datasets, we provide compelling evidence of scButterfly’s superiority over baseline methods in preserving cellular heterogeneity while translating datasets of various contexts and in revealing cell type-specific biological insights. Besides, we demonstrate the extensive applications of scButterfly for integrative multi-omics analysis of single-modality data, data enhancement of poor-quality single-cell multi-omics, and automatic cell type annotation of scATAC-seq data. Moreover, scButterfly can be generalized to unpaired data training, perturbation-response analysis, and consecutive translation.

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Section: Resultsmentioning

confidence: 99%

scButterfly: a versatile single-cell cross-modality translation method via dual-aligned variational autoencoders

Cao,

Zhao,

Tang

et al. 2024

Nat Commun

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“…In AD therapy, anti-inflammatory therapy with topical corticosteroids or topical calcineurin inhibitors is a major element and represents the most effective and first-line treatment for reducing inflammation . However, these topical treatments are associated with long-term use side effects, including skin atrophy, dermatoglyphia, telangiectasia, pigmentation, and stabbing pain. , Dry skin is a frequent clinical complication associated with numerous skin diseases, including psoriasis and atopic dermatitis (AD), as well as various systemic diseases . Therefore, the application of moisturizers plays an indispensable role in the management of AD.…”

Section: Introductionmentioning

confidence: 99%

“… 10 , 11 Dry skin is a frequent clinical complication associated with numerous skin diseases, including psoriasis and atopic dermatitis (AD), as well as various systemic diseases. 12 Therefore, the application of moisturizers plays an indispensable role in the management of AD. For patients with moderate to severe AD, a combination of systemic treatments is often necessary, encompassing corticosteroids, immunosuppressants, and biologics.…”

Section: Introductionmentioning

confidence: 99%

Liangxue-Qushi-Zhiyang Decoction Ameliorates DNCB-Induced Atopic Dermatitis in Mice through the MAPK Signaling Pathway Based on Network Pharmacology

Zhang,

Niu

et al. 2024

ACS Omega

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The traditional prescription of Liangxue-Qushi-Zhiyang decoction (LQZ) has been demonstrated to be efficacious in treating atopic dermatitis (AD), a chronic inflammatory skin disorder marked by intense itching, redness, rashes, and skin thickening. Nevertheless, there has been an inadequate systematic exploration of the potential targets, biological processes, and pathways for AD treatment through LQZ. The study objective was to evaluate the efficacy and possible mechanism of LQZ in AD mice. In our study, we identified the primary compounds of LQZ, analyzed hub targets, and constructed a network. Subsequently, the predicted mechanisms of LQZ in AD were experimentally studied and validated in vivo, as determined by network pharmacological analysis. A total of 80 serum components of LQZ were identified through ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS), among which 49 compounds were absorbed into the bloodstream. Our results indicated that LQZ targets six putative key factors in the MAPK signaling pathway, which play essential roles in AD, namely, EGFR, p-MAPK1/3, p-MAPK14, IL-1β, IL-6, and TNF-α. We observed spleen coefficient, dermatitis scores, and ear thickness were all downregulated in 2,4dinitrochlorobenzene (DNCB)-induced mice after LQZ treatment. Histological analysis of the dorsal and ear skin further revealed that LQZ significantly decreased skin inflammation, epidermal thickness, and mast cell numbers compared to the DNCB group. Our study demonstrated the effectiveness of LQZ in reducing epidermal and dermal damage in a mouse model of AD. Furthermore, our findings suggest that downregulating the MAPK signaling pathway could be a potential therapeutic strategy for the treatment of AD.

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“…In recent years, single-cell RNA sequencing ( scRNA-seq ) has become a powerful tool for dissecting cellular heterogeneity ( Flotho et al, 2023 ; He et al, 2023a ; Qu et al, 2023 ). Currently, scRNA-seq has been applied in the field of skin biology research in species such as humans ( Joost et al, 2016 ; Wang et al, 2020 ), mice ( Huang et al, 2023 ), rats ( Zhang et al, 2023a ), and fish ( Peloggia et al, 2021 ). Despite its widespread use, scRNA-seq has not been explored in avian skin research.…”

Section: Introductionmentioning

confidence: 99%

A single-cell transcriptome atlas of Lueyang black-bone chicken skin

Wang,

Xue,

Tian

et al. 2024

Poultry Science

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Cux1+ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics

Cited by 3 publications

References 63 publications

scButterfly: a versatile single-cell cross-modality translation method via dual-aligned variational autoencoders

scButterfly: a versatile single-cell cross-modality translation method via dual-aligned variational autoencoders

Liangxue-Qushi-Zhiyang Decoction Ameliorates DNCB-Induced Atopic Dermatitis in Mice through the MAPK Signaling Pathway Based on Network Pharmacology

A single-cell transcriptome atlas of Lueyang black-bone chicken skin

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