CXCL16 expression during post-traumatic osteoarthritis development: implications for mesenchymal stem cell recruitment

Abstract

treated in OA milieu showed an increase of CXCR3, CCR3 and IL6R and a decrease of CCR1 receptors. Blocking experiments demonstrate that CXCL10/IP10 was the only chemokine of the OA milieu down-modulated after treatment with GMP-ASC. We confirmed that CXCL10/IP10 was mainly positive on synovial macrophages. Conclusions: In conclusion, our study demonstrates a novel specific effect of OA milieu on both GMP-ASC migration and cytokine receptor expression that were strictly dependent on an inflammatory environment and hypoxic condition. Therefore, only the use of a specific culture OA environment allows to specifically define the real therapeutic effect of GMP-ASC, suggesting that their specific recruitment to synovial macrophages is partially dependent on CXCL10/IP10-CXCR3 axis. This is the first study that focuses on an adequate GMP-ASC injection environment, presenting novel indications that contribute to understand the role of OA milieu on GMP-ASC .