Cyanine dye labeling reagents for sulfhydryl groups

Эрнст, Л. К.; Gupta, Reshma; Mujumdar, Ratnakar B.; Waggoner, Alan S.

doi:10.1002/cyto.990100103

Cited by 207 publications

(127 citation statements)

References 23 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…Inc.1,1 -diethyl-3,3,3 ,3 -tetramethylcarbocyanine perchlorate (CY-5) and 1-sulfopropyl-1 -ethyl-3,3,3 ,3 -tetramethylcarbocyanine (CY-10) were synthesized in our laboratory based on published procedures [18,19].…”

Section: Methodsmentioning

confidence: 99%

Counterion effects in cyanine heterojunction photovoltaic devices

et al. 2005

View full text Add to dashboard Cite

We investigated cyanine heterojunction photovoltaic devices using carbocyanine dyes as donors and buckminsterfullerene (C60) as acceptor. In particular, we focused on the influence of cyanine counterions on the photovoltaic device characteristics. It was found that counterions can be displaced in the applied electric field and give rise to important hystereses in the current-voltage characteristics, which are related to charge injection processes at electrode and organic heterointerfaces. Mobile counterions have also a drastic effect on the photocurrent spectrum and are responsible for an important C60 contribution at the organic heterojunction between cyanine and C60. If the counterion is covalently linked to the cyanine dye, the C60 contribution in the blue spectral domain can not be observed. C 2005 Springer Science + Business Media, Inc.

show abstract

Section: Methodsmentioning

confidence: 99%

Counterion effects in cyanine heterojunction photovoltaic devices

et al. 2005

View full text Add to dashboard Cite

show abstract

“…al. 30 Briefly, 2,3,3-trimethyl-3H-indole (4.8 g) and 1,4-butanesultone (4.2 ml) were heated for 18 hours at 110°C. The resulting solid was triturated with ethyl acetate (50 ml).…”

Section: -(233-trimethyl-3h-indolium-1-yl)butane-1-sulfonate (4)mentioning

confidence: 99%

Enhanced aqueous solubility of long wavelength voltage-sensitive dyes by covalent attachment of polyethylene glycol

et al. 2007

Self Cite

View full text Add to dashboard Cite

Long wavelength voltage-sensitive dyes (VSDs) called Pittsburgh (PGH) dyes were recently synthesized by coupling various heterocyclic groups to a styryl-thiophene intermediate forming extended, partially rigidized chromophores. Unlike most styryl VSDs, dyes with a sulfonic acid anchor directly attached to the chromophore showed no solvatochromic absorption shifts. The limited water solubility of many long wavelength VSDs requires the use of surfactants to transport the dye through aqueous media and effectively label biological membranes. Here, we tested the chemical substitution of the sulfonic acid moiety with polyethyleneglycol (PEG) chains ranging from MW 750 to 5000, to overcome the poor solubility of VSDs while retaining their properties as VSDs. The chemical synthesis of PGH dyes and their PEG derivatives are described. The PEGderivatives were soluble in aqueous solutions (> 1 mM) and still reported membrane potential changes. In frog and mouse hearts, the voltage sensitivity (ΔF/F per action potential) and spectral properties of PEG dyes were the same as the sulfonated analogs. Thus, the solubility of VSDs can be considerably improved with small polyethyleneglycol chains and can provide an effective approach to improve staining of excitable tissues and optical recordings of membrane potential.

show abstract

“…Imaging cytometry lacks the side and forward scatter parameters which are used in flow systems for gating on cells, but this is compensated by the greater ease of adding further fluorophores, with appropriate specific antibodies. In flow systems this requires extra photodetectors, but in imaging the limitation is only the number of fluorophores which may be distinguished reliably within the available spectral range, and this is improved by using fluorophores which extend into the far red and near infra-red (5,16,17). Using Hoechst or DAPI, cell DNA content could be determined in addition to the presence of three to four additional antigen markers labeled with fluorophores emitting in the visible.…”

Section: Image Cytometry Vs Flow Cytometrymentioning

confidence: 99%

Imaging cytometry by multiparameter fluorescence

et al. 1991

Self Cite

View full text Add to dashboard Cite

A system is described for performing multicolor fluorescence image cytometry of cell preparations. After the setting up stage, the operation is automatic: the microscope fields are found and focused; then images are acquired for each fluorophore, corrected and analyzed, without any operator interaction. Human peripheral blood lymphocytes on microscope slides were used as a test system. In these experiments, three fluorescent antibodies were used to identify lymphocyte sub-populations, and a DNA content probe was used to identify all nucleated cells. The cell subset percentages determined by image cytometry were comparable to percentages obtained when cells from the same preparation were analyzed by flow cytometry. Multicolor fluorescence imaging cytometry can potentially be extended to the analysis of cells in smears, fine needle biopsies, imprints, and tissue sections. Key terms: Image processing, quantitation, lymphocytesMulticolor fluorescence image analysis of cells present in tissue sections, smears, and imprints by microscopy offers a potentially powerful approach for correlating the locations of cells as well as their quantity in the two-or three-dimensional space of the sample. Intracellular and surface antigens, nucleic acid sequences from RNA and DNA, DNA content, and chromo/fluorogenic substrate precipitates are examples of parameters that could be related to sample morphology. In order for image cytometry to realize this potential, instrumentation with optimal light sources, filter sets, and detectors must be combined with software that permits identification of stained structures, accurate quantitation of the fluorescence or absorption signals, morphological analysis, and data reduction and presentation.While single-color fluorescence image cytometry of cells has been carried out previously in a number of laboratories (8,9,12,14,19), the optics, probes, hardware, and computers have recently advanced significantly, and now multicolor quantitative analysis offers enormous potential €or analysis of fixed and living cells and tissues. For example, the availability of high-resolution cooled CCD cameras with a broad spectral range, minimal spatial distortion, excellent linearity, and a wide dynamic range have made quantitative fluorescence analysis more simple than before (7,10,18). Laser scanning microscopes equipped with multiple excitation lines and detectors provide an alternative approach for multicolor immunofluorescence (2). Also, improvements in interference filters have made it possible to image multiple fluorophores that emit with narrow Stokes shifts in the visible region of the spectrum (6). Furthermore, additional fluorophores have become available at new wavelengths, such as CY5 (171, which emits in the deep red region of the spectrum where CCD cameras are very sensitive. And finally, imaging computers continue to be more powerful and cost effective year by year.Here we have developed software and methods for the four-color analysis of lymphocyte subsets on microscope slides. This work repre...

show abstract

Cyanine dye labeling reagents for sulfhydryl groups

Cited by 207 publications

References 23 publications

Counterion effects in cyanine heterojunction photovoltaic devices

Counterion effects in cyanine heterojunction photovoltaic devices

Enhanced aqueous solubility of long wavelength voltage-sensitive dyes by covalent attachment of polyethylene glycol

Imaging cytometry by multiparameter fluorescence

Contact Info

Product

Resources

About