Cyanophage dynamics in a tropical urban freshwater lake

Zhang, Dong; Te, Shu Harn; He, Yiliang; Gin, Karina Yew-Hoong

doi:10.1016/j.ecolind.2022.109257

Cited by 1 publication

(1 citation statement)

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“…This relationship seems to denote a constant presence of cyanophage which may not affect the duration or intensity of the bloom. Single year quantification of Microcystis cyanophages in Singapore showed similar relationships between host cell concentrations and host-associated viral loads between mid-July and early-August, noting that despite the high host-associated load, there was no corresponding high free phage load that would indicate active lytic infections ( Zhang et al, 2022 ). Similarly, despite being a log or two less abundant than its host, Microcystis , Ma-LMM01 concentrations in the host cell fraction mirrored host concentrations in a Japanese pond, and high concentrations in the host fraction did not necessarily signal high concentrations in the free phage fraction ( Kimura et al, 2012 ).…”

Section: Discussionmentioning

confidence: 94%

Multi-year molecular quantification and ‘omics analysis of Planktothrix-specific cyanophage sequences from Sandusky Bay, Lake Erie

et al. 2023

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IntroductionPlanktothrix agardhii is a microcystin-producing cyanobacterium found in Sandusky Bay, a shallow and turbid embayment of Lake Erie. Previous work in other systems has indicated that cyanophages are an important natural control factor of harmful algal blooms. Currently, there are few cyanophages that are known to infect P. agardhii, with the best-known being PaV-LD, a tail-less cyanophage isolated from Lake Donghu, China. Presented here is a molecular characterization of Planktothrix specific cyanophages in Sandusky Bay.Methods and ResultsPutative Planktothrix-specific viral sequences from metagenomic data from the bay in 2013, 2018, and 2019 were identified by two approaches: homology to known phage PaV-LD, or through matching CRISPR spacer sequences with Planktothrix host genomes. Several contigs were identified as having viral signatures, either related to PaV-LD or potentially novel sequences. Transcriptomic data from 2015, 2018, and 2019 were also employed for the further identification of cyanophages, as well as gene expression of select viral sequences. Finally, viral quantification was tested using qPCR in 2015–2019 for PaV-LD like cyanophages to identify the relationship between presence and gene expression of these cyanophages. Notably, while PaV-LD like cyanophages were in high abundance over the course of multiple years (qPCR), transcriptomic analysis revealed only low levels of viral gene expression.DiscussionThis work aims to provide a broader understanding of Planktothrix cyanophage diversity with the goals of teasing apart the role of cyanophages in the control and regulation of harmful algal blooms and designing monitoring methodology for potential toxin-releasing lysis events.

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Section: Discussionmentioning

confidence: 94%