Cyathuscavins A, B, and C, new free radical scavengers with DNA protection activity from the Basidiomycete Cyathus stercoreus

Kang, Hahk‐Soo; Kim, Kyoung-Rok; Jun, Eun-Mi; Park, Soon-Hye; Lee, Tae-Soo; Suh, Joo‐Won; Kim, Jong-Pyung

doi:10.1016/j.bmcl.2008.05.110

Cited by 28 publications

(20 citation statements)

References 15 publications

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“…Searching and identifying natural and safe antioxidants, especially of plant origin, have been notably increased in recent years [26,27]. Assays based on the use of O 2 −• and ·OH, DPPH, ABTS +· , and N, N -dimethyl- p -phenylenediamine dihydrochloride cation radical (DMPD +· ) are among the most popular spectrophotometric methods for determination of the antioxidant capacity of foods, beverages and vegetable extracts [26–32].…”

Section: Introductionmentioning

confidence: 99%

“…DPPH has been widely used to evaluate the free radical scavenging effectiveness of various antioxidant substances [27,28,30]. In the DPPH assay, the antioxidants are able to donate a hydrogen to reduce the stable radical DPPH to the yellow-colored non-radical diphenyl-picrylhydrazine (DPPH-H).…”

Section: Introductionmentioning

confidence: 99%

“…Treating these conditions with antioxidants is of growing interest. At the same time, the DNA or plasmid damage has been used as models for the study and identification of antioxidants [27,32,62,66,91,126]. A typical research model of DNA damage caused by Cu + induced ·OH has been developed [62,63].…”

Section: Introductionmentioning

confidence: 99%

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Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems

Lü

Lin

Yao

et al. 2010

J Cellular Molecular Medi

1,021

480

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Free radicals derived from oxygen, nitrogen and sulphur molecules in the biological system are highly active to react with other molecules due to their unpaired electrons. These radicals are important part of groups of molecules called reactive oxygen/nitrogen species (ROS/RNS), which are produced during cellular metabolism and functional activities and have important roles in cell signalling, apoptosis, gene expression and ion transportation. However, excessive ROS attack bases in nucleic acids, amino acid side chains in proteins and double bonds in unsaturated fatty acids, and cause oxidative stress, which can damage DNA, RNA, proteins and lipids resulting in an increased risk for cardiovascular disease, cancer, autism and other diseases. Intracellular antioxidant enzymes and intake of dietary antioxidants may help to maintain an adequate antioxidant status in the body. In the past decades, new molecular techniques, cell cultures and animal models have been established to study the effects and mechanisms of antioxidants on ROS. The chemical and molecular approaches have been used to study the mechanism and kinetics of antioxidants and to identify new potent antioxidants. Antioxidants can decrease the oxidative damage directly via reacting with free radicals or indirectly by inhibiting the activity or expression of free radical generating enzymes or enhancing the activity or expression of intracellular antioxidant enzymes. The new chemical and cell-free biological system has been applied in dissecting the molecular action of antioxidants. This review focuses on the research approaches that have been used to study oxidative stress and antioxidants in lipid peroxidation, DNA damage, protein modification as well as enzyme activity, with emphasis on the chemical and cell-free biological system.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems

Lü

Lin

Yao

et al. 2010

J Cellular Molecular Medi

1,021

480

View full text Add to dashboard Cite

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“…Similarly, the percentage of inhibition of ABTS radicals was found to be in the range of 40 to 75.5%. Among the several in vitro antioxidant assays, DPPH * and ABTS *+ assays have been widely used as more reliable methods in determining the free radical scavenging efficacy of unknown compounds [54,55]. The antiradical activity assay is based on the reduction of DPPH * in methanolic solution.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Evaluation of in Vitro Antioxidant Potential of Immature Palmyra Palm (Borassus Flabellifer Linn.) Fruits

Renuka

Devi

Subramanian

2018

Int J Pharm Pharm Sci

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Objective: The present study was aimed to evaluate the in vitro antioxidant properties of immature Palmyra palm fruits which have been traditionally used for the treatment of diabetes. The qualitative phytochemical screening and quantitative estimation of total phenolic and flavonoids contents in the ethanolic extract were performed to substantiate the antioxidant and medicinal claims. Methods:Immature palmyra palm fruits were collected, authenticated, dried and powdered in an electrical grinder. The powdered fruits were delipidated in petroleum ether and soxhilation using ethanol to extract the active ingredients. Qualitative phytochemical screening, total phenolic and flavonoid contents were carried out by established methods. The in vitro antioxidant potentials were performed by diphenyl-2-picrylhydrazyl, azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, nitric oxide and superoxide anion scavenging assays. Results:The phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, phytosterols, triterpenoids and phenols in the immature palmyra palm fruits extract. The total phenolic and flavonoid contents in the fruits extract was found to be 104.00±0.02 μg gallic acid equivalents/100 mg of fruits extract and 98.45±0.03 μg quercetin equivalents/100 mg, respectively. The percentage inhibition of DPPH radicals range from 35 to 70% at a concentration ranges from 200-1000μg/ml. Similarly, the percentage of inhibition of ABTS radicals was found to be in the range of 40 to 75.5%. The nitric oxide scavenging activity of the fruits extract ranges from 45 to 76% whereas the superoxide radical scavenging activity ranges from 43 to 83%. Conclusion:The observed significant free radical scavenging activity along with increased total phenolic as well as flavonoid contents suggest that the immature fruits may be considered as a potential source for the identification of pharmacologically active phytochemicals capable of controlling oxidative stress.

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“…[32,33] In brief, damage solution was prepared by adding (1 mM •OH) was prepared by adding 1.6 µl of 30% H 2 O 2 into 50 ml of 1 mM FeSO 4 . Total reaction solution consists of 5 µl plasmid DNA (pUC19), 5 µl of CNB-001 (20, 40, 60, 80 µg/ml), 5 µl of damage solution and irradiated in ultraviolet (UV) by placing on UV transilluminator (300 nm).…”

Section: Dna Protecting Activitymentioning

confidence: 99%

In vitro antioxidant potential and deoxyribonucleic acid protecting activity of CNB-001, a novel pyrazole derivative of curcumin

Jayaraj

Elangovan

2014

Chron Young Sci

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Background: Free radicals are underpinned to initiate cascade of toxic events leading to oxidative stress and resultant cell death in many neurodegenerative disorders. Now-a-days antioxidants have become mandatory in the treatment of various diseases apart from the drug's modes of action. CNB-001, a novel hybrid molecule synthesized by combining curcumin and cyclohexyl bisphenol A is known to possess various biological activities, but the antioxidative property of the compound has not yet been elucidated. Aim: The present study is aimed to analyze various free radicals scavenging by employing in vitro antioxidant assays and to evaluate the deoxyribonucleic acid (DNA) protecting the ability of CNB-001 against hydroxyl radicals. Materials and methods: The in vitro antioxidant potential of CNB-001 was evaluated by analyzing its ability to scavenge DPPH, ABTS, nitric oxide, superoxide, hydrogen peroxide, superoxide anion, hydroxyl, hydrogen peroxide radicals and reducing power using spectroscopic method. The DNA protecting activity of CNB-001 was also evaluated on pUC19 plasmid DNA subjected to hydroxyl radicals using standard agarose gel electrophoresis. Results: From the assays, it was observed that CNB-001 scavenged free radicals effectively in a dose dependent manner. CNB-001 scavenged 2,2-diphenyl-1-picrylhydrazyl (IC50 = 44.99 µg/ml), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (IC50 = 17.99 µg/ml), nitric oxide (IC50 = 1.36 µg/ml), superoxide radical (IC50 = 77.17 µg/ml), hydrogen peroxide (IC50 = 492.7 µg/ml), superoxide (IC50 = 36.92 µg/ml) and hydroxyl (IC50 = 456.5 µg/ml) radicals effectively and the reducing power was found to be 11.53 µg/ml. CNB-001 showed considerable protecting activity against plasmid DNA (pUC19) strand scission by •OH at dose dependent manner. Conclusion: Results from these assays concluded that CNB-001 has a good antioxidant potential by reducing reactive oxygen and reactive nitrogen radicals and it showed significant protecting activity against DNA scission by hydroxyl radicals. Hence, CNB-001 can be further developed as potential drug for free radical induced neurodegenerative disorders.

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Cyathuscavins A, B, and C, new free radical scavengers with DNA protection activity from the Basidiomycete Cyathus stercoreus

Cited by 28 publications

References 15 publications

Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems

Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems

Phytochemical Screening and Evaluation of in Vitro Antioxidant Potential of Immature Palmyra Palm (Borassus Flabellifer Linn.) Fruits

In vitro antioxidant potential and deoxyribonucleic acid protecting activity of CNB-001, a novel pyrazole derivative of curcumin

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