Cyclamen persicum: Methanolic Extract Using Gas Chromatography-Mass Spectrometry (GC-MS) Technique

Ibraheam, Israa Adnan; Hameed, Sundus; Hussein, Haider Mashkoor

doi:10.25258/ijpqa.v8i04.10546

Cited by 6 publications

(5 citation statements)

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“…The fruit is used in nearly every home in the tropics, mainly to flavour food, but also to prepare drinks and for a variety of medicinal applications. The antifungal activity of the plant have been attributed to the presence of monoterpenes and the plant is currently used as a fungicide for citrus fruit crop, and it has also been suggested that the plant may be a potential candidate used for the protection of food and feeds from toxigenic fungal growth as well as their aflatoxin contamination [1][2][3][4][5][6][7][8][9] . It has been traditionally used in the management of several diseases and has the prospects of being developed into useful drugs.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Methanolic Fruit Extract of Citrus aurantifolia Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

Hameed¹,

Shareefi²,

Hameed³

2018

Ind. Jour. of Publ. Health Rese. & Develop.

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The objectives of this study were analysis of the secondary metabolite products and evaluation antibacterial and antifungal activity. Bioactives are chemical compounds often referred to as secondary metabolites. Twenty nine bioactive compounds were identified in the methanolic extract of Citrus aurantifolia. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Citrus aurantifolia revealed the existence of the Thieno[2,3-c] furan-3-carbonitrile, 2-amino-4,6-dihydro-4

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Section: Introductionmentioning

confidence: 99%

Analysis of Methanolic Fruit Extract of Citrus aurantifolia Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

Hameed¹,

Shareefi²,

Hameed³

2018

Ind. Jour. of Publ. Health Rese. & Develop.

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“…The GC-MS analysis of fractions 19–26 (25–30% EtOAc in n -hexane as eluent) showed the presence of two peaks as shown in the chromatogram ( Figure 2 ). By comparing the mass spectra ( Figure 3 ) of the composition at the retention time of 4 min with the NIST library, compound 4 was identified as a pregnane derivative named as 3,9-epoxypregn-16-ene-14,20-diol,7,11,18-triacetoxy-3-OCH 3 , previously reported from the GC-MS of CH 3 OH extract of Cyclamen persicum [ 39 ].…”

Section: Resultsmentioning

confidence: 94%

In Vitro Antibacterial, Antioxidant, Cytotoxicity Activity, and In Silico Molecular Modelling of Compounds Isolated from Roots of Hydnora johannis

Degfie,

Endale,

Aliye

et al. 2024

Biochemistry Research International

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The plant Hydnora johannis has been utilized in folk medicine. Analyzing phytochemical composition of dichloromethane/methanol (1 : 1) root part of Hydnora johannis gave oleic acid (1), caffeic acid‐2‐hydroxynonylester (2), catechin (3), and a pregnane derivative (4). NMR spectroscopy was used to characterize compounds 1–3, while compound 4 was identified through GC‐MS analysis and literature comparison. The cytotoxicity of extracts from roots of H. johannis was conducted against MCF‐7 cell lines (human breast cancer) by MTT assay. According to the cytotoxicity study, n‐hexane extract exhibited a high level of toxicity with 28.9 ± 5.6% cell viability. Antibacterial activity was tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pyogen. The highest bacterial growth mean inhibition zone was measured for catechin (3) (13.72 ± 0.05 mm)) against P. aeruginosa at 0.25 mg/mL and acceptable related to standard. Antioxidant activity was studied by the DPPH assay. Based on the data from the antioxidant study, DCM/MeOH extract (70.32%) and catechin (3) showed good antioxidant activity (65.61%) (IC50 0.25 μg/mL) relative to that of the positive control (78.21%, IC50 0.014 μg/mL) at 12.5 μg/mL. In each docking pose, catechin (3) scored higher binding affinity of −7.9, −7.2, and −6.4 kcal/mol towards PqsA, DNA gyraseB, and S. aureus PK, respectively, compared to amoxicillin (−8.1, −6.1, and −6.4 kcal/mol). All five Lipinski rules were obeyed by compounds 1–3, which showed an acceptable drug resemblance. The lipophilicity was computed as less than five (1.47–4.01) indicating a lipophilic property. Catechin (3) obeys Veber’s rule implying its good oral bioavailability. Binding affinity scores of catechin (3)‐protein interactions are in line with those from in vitro tests, indicating its potential antibacterial effect. The obtained cytotoxicity and antibacterial activity results support the utilization of H. johannis in folk medicine.

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“…Because of its solvent-free nature and low toxicity, plant-based nanoparticle production has gained popularity in recent years. Furthermore, their synthesis is much quicker and less costly [9,10]. Bacteria, yeast, and fungi have been demonstrated to produce AgNP during the biosynthetic process [11,13].…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of non-cytotoxic silver nanoparticles using Solanum nigrum leaves extract with antibacterial properties

Anushaa¹,

Agrawal²,

G³

et al. 2021

GSC Biol. and Pharm. Sci.

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Green synthesizes of silver nanoparticles is a promising method in material science and nanotechnology today. Silver nanoparticles (AgNP) were synthesized using a high-efficiency, cost-effective green and simple method at room temperature using Solanum nigrum leaves extract. The biosynthesized AgNP was validated and classified using spectroscopy profiles from ultraviolet-visible spectroscopy, scanning electron microscope, Fourier-transform infrared spectroscopy and x-ray diffraction. FTIR spectra indicated the presence of biological molecules in AgNP synthesis and UV-visible spectra revealed a surface resonance peak of 420 nm corresponding to AgNP formation. The antimicrobial potential of these synthesized nanoparticles is evaluated against the bacteria Escherichia coli (MCC 2079) and Staphylococcus aureus (MCC 2408), both of which are important foodborne pathogens. Ciprofloxacin and Clotrimazole were used as positive controls antibiotic. The nanoparticles were shown to have strong antibacterial efficacy against the strains examined. Their antibacterial function allows them to be included in antimicrobial formulations. By using the MTT assay, the biocompatibility of Solanum nigrum silver nanoparticles were found non-toxic to mouse fibroblast cell lines (L929 is one of the first in continuous culture to be created. The L strain was generated from a male C3H mouse's usual subcutaneous areolar and adipose tissue.) at lower concentrations.

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Cyclamen persicum: Methanolic Extract Using Gas Chromatography-Mass Spectrometry (GC-MS) Technique

Cited by 6 publications

References 0 publications

Analysis of Methanolic Fruit Extract of Citrus aurantifolia Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

Analysis of Methanolic Fruit Extract of Citrus aurantifolia Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

In Vitro Antibacterial, Antioxidant, Cytotoxicity Activity, and In Silico Molecular Modelling of Compounds Isolated from Roots of Hydnora johannis

Green synthesis of non-cytotoxic silver nanoparticles using Solanum nigrum leaves extract with antibacterial properties

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