Cyclin A expression and growth in suspension can be uncoupled from p27 deregulation and extracellular signal-regulated kinase activity in cells transformed by bovine papillomavirus type 4 E5

Zago, Manola; Campo, M. Saveria; O'Brien, Vincent

doi:10.1099/vir.0.80313-0

Cited by 8 publications

(9 citation statements)

References 43 publications

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“…These encode genes for replication (Mohr et al 1990;Kurg et al 2009;Liu et al 2010), genome encapsidation (L1 and L2 are the major and minor capsid proteins, respectively) and oncogenesis in animals (Tong et al 1997;Zimmermann et al 2000;Ashrafi et al 2002;Zago et al 2004). Unlike the other eight characterized types of BPV (Nasir and Campo 2008), BPV-2 and to a greater extent BPV-1 are not as species specific and have been reported to infect and cause disease in a variety of bovids and equids (Nasir and Campo 2008).…”

Section: Introductionmentioning

confidence: 96%

In planta production of a candidate vaccine against bovine papillomavirus type 1

Love

Chapman

Matić

et al. 2012

Planta

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Bovine papillomavirus type 1 (BPV-1) is an economically important virus that induces tumourigenic pathologies in horses and cows. Given that the BPV-1 L1 major coat protein can self-assemble into highly immunogenic higher-order structures, we transiently expressed it in Nicotiana benthamiana as a prelude to producing a candidate vaccine. It was found that plant codon optimization of L1 gave higher levels of expression than its non-optimized counterpart. Following protein extraction, we obtained high yields (183 mg/kg fresh weight leaf tissue) of relatively pure L1, which had self-assembled into virus-like particles (VLPs). We found that these VLPs elicited a highly specific and strong immune response, and therefore they may have utility as a potential vaccine. This is the first report demonstrating the viable production of a candidate BPV vaccine protein in plants.

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Section: Introductionmentioning

confidence: 96%

In planta production of a candidate vaccine against bovine papillomavirus type 1

Love

Chapman

Matić

et al. 2012

Planta

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“…This hypothesis however requires confirmation and other explanations are possible. BPV E5 is not present in the nucleus (Burkhardt et al, 1989;Pennie et al, 1993;Zago et al, 2004) and its effect on transcription is therefore not direct. Rather, BPV E5 interferes with several signal transduction pathways (DiMaio and Mattoon, 2001;O'Brien et al, 2001;Zago et al, 2004;Grindlay et al, 2005), and it is therefore likely that inhibition of the BoLa promoter/enhancer is achieved by interference with one or more of these pathways.…”

Section: Inhibition Of Transcription Of the Mhc Class I Hc Gene By Bpmentioning

confidence: 99%

“…BPV E5 is not present in the nucleus (Burkhardt et al, 1989;Pennie et al, 1993;Zago et al, 2004) and its effect on transcription is therefore not direct. Rather, BPV E5 interferes with several signal transduction pathways (DiMaio and Mattoon, 2001;O'Brien et al, 2001;Zago et al, 2004;Grindlay et al, 2005), and it is therefore likely that inhibition of the BoLa promoter/enhancer is achieved by interference with one or more of these pathways. We have already shown that BPV-4 E5 affects the expression of cyclin A by acting indirectly on the transcriptional promoter of the cyclin A gene (O'Brien et al, 1999;Grindlay et al, 2005) and BPV-4 E5 inhibition of the BoLa promoter/enhancer is another example of E5 interference with transcription regulation.…”

Section: Inhibition Of Transcription Of the Mhc Class I Hc Gene By Bpmentioning

confidence: 99%

“…Residue 33 is not present in either 1-E5T or 4-E5T (O'Brien et al, 1999), and we cannot rule out that it plays a role in the interaction with HC. However, although we have not investigated any interaction between BPV-4 E5 and PDGF receptor, cell transformation by BPV-4 E5 does not appear to depend on signalling from growth factor receptors (Zago et al, 2004;Grindlay et al, 2005), and it is therefore unlikely that receptor binding would compete with HC binding.…”

Section: Bpv-4 E5 and Mhc Class I B Marchetti Et Almentioning

confidence: 99%

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The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus

et al. 2005

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BPV-4 E5 inhibits transcription of the bovine MHC class I heavy chain (HC) gene, increases degradation of HC and downregulates surface expression of MHC class I by retaining the complex in the Golgi apparatus (GA). Here we report that transcription inhibition can be alleviated by interferon treatment and the degradation of HC can be reversed by treatment with inhibitors of proteasomes and lysosomes. However, the inhibition of transport of MHC class I to the cell surface is irreversible. We show that E5 is capable of physically interacting with HC. Together with the inhibition of the vacuolar ATPase (due to the interaction between E5 and 16k subunit c), the interaction between E5 and HC is likely to be responsible for retention of MHC class I in the GA. C-terminus deletion mutants of E5 are incapable of either downregulating surface MHC class I or interacting with HC, establishing that the C-terminus domain of E5 is important in the inhibition of MHC class I.

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“…It has been proposed that BPV-4 E5 induces a coordinated increase in p27 Kip1 and cyclin D1-cdk4, which, together with increased cyclin A-cdk activity, allow continued cell proliferation as well as anchorage independent growth of cultured cells [93,142].…”

Section: E5mentioning

confidence: 99%

Bovine papillomaviruses, papillomas and cancer in cattle

Borzacchiello¹,

Roperto²

2008

Vet. Res.

173

149

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-Bovine papillomaviruses (BPV) are DNA oncogenic viruses inducing hyperplastic benign lesions of both cutaneous and mucosal epithelia in cattle. Ten (BPV 1-10) different viral genotypes have been characterised so far. BPV 1-10 are all strictly species-specific but BPV 1/2 may also infect equids inducing fibroblastic tumours. These benign lesions generally regress but may also occasionally persist, leading to a high risk of evolving into cancer, particularly in the presence of environmental carcinogenic co-factors. Among these, bracken fern is the most extensively studied. The synergism between immunosuppressants and carcinogenic principles from bracken fern and the virus has been experimentally demonstrated for both urinary bladder and alimentary canal cancer in cows whose diets were based on this plant. BPV associated tumours have veterinary and agricultural relevance in their own right, although they have also been studied as a relevant model of Human papillomavirus (HPV). Recent insights into BPV biology have paved the way to new fields of speculation on the role of these viruses in neoplastic transformation of cells other than epithelial ones. This review will briefly summarise BPV genome organization, will describe in greater detail the functions of viral oncoproteins, the interaction between the virus and co-carcinogens in tumour development; relevant aspects of immunity and vaccines will also be discussed.

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Cyclin A expression and growth in suspension can be uncoupled from p27 deregulation and extracellular signal-regulated kinase activity in cells transformed by bovine papillomavirus type 4 E5

Cited by 8 publications

References 43 publications

In planta production of a candidate vaccine against bovine papillomavirus type 1

In planta production of a candidate vaccine against bovine papillomavirus type 1

The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus

Bovine papillomaviruses, papillomas and cancer in cattle

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